Variation in susceptibility to microbial lignin oxidation in a set of wheat straw cultivarsinfluence of genetic, seasonal and environmental factors

Authors Organisations
  • Marina Konstantopoulou(Author)
    University of Warwick
  • Patrick Slator(Author)
    University of Warwick
  • Charles Taylor(Author)
    University of Warwick
  • Elizabeth Wellington(Author)
    University of Warwick
  • Gordon Allison(Author)
  • Andrea Harper(Author)
    University of York
  • Ian Bancroft(Author)
    University of York
  • Timothy Bugg(Author)
    University of Warwick
Type Article
Original languageEnglish
Pages (from-to)493-507
Number of pages15
JournalNordic Pulp and Paper Research Journal
Volume32
Issue number4
DOI
Publication statusPublished - 19 Jul 2018
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Abstract

An alkali lignin preparation from wheat straw was found to be compatible with a previously developed nitrated lignin assay for microbial lignin oxidation, allowing comparison of the susceptibility of different wheat lignins to microbial oxidation. Alkali lignin was prepared from a panel of 18 wheat varieties, and was analysed by FT-IR spectroscopy, revealing minor variations in the lignin structure. Samples of nitrated alkali lignin were assayed against five lignin degrading bacteria: Streptomyces viridosporus, Rhodococcus jostii RHA1, Pseudomonas putida mt-2, Microbacterium phyllosphaerae, and Sphingobacterium sp. T2. Up to 5-fold variation in rates of lignin oxidation
were observed between different wheat varieties, and between different bacterial strain, with Sphingobacterium sp. T2 showing consistently highest absorbance changes. Testing of wheat varieties from two consecutive harvests, and from different locations, revealed that the observed variation was due to a ombination of genetic, seasonal and environmental factors, but that some wheat varieties showed consistently higher rates of oxidation. Samples of wheat straw treated with Sphingobacterium sp. T2 were analysed by FT-IR spectroscopy after 7 and 14 days’ treatment, showing changes in lignin structure versus time, consistent with lignin breakdown.

Keywords

  • Microbial lignin degradation, Wheat straw, Sphingobacterium, Rhodococcus jostii RHA1, Pseudomonas putida