Using 'Omic Approaches to Compare Temporal Bacterial Colonization of Lolium perenne, Lotus corniculatus and Trifolium pratense in the Rumen

Type Article
Original languageEnglish
Article number2184
JournalFrontiers in Microbiology
Volume9
DOI
Publication statusPublished - 19 Sep 2018
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Abstract

Understanding rumen plant-microbe interactions is central for development of novel methodologies allowing improvements in ruminant nutrient use efficiency. This study investigated rumen bacterial colonisation of fresh plant material and changes in plant chemistry over a period of 24 h period using three different fresh forages: Lolium perenne (perennial ryegrass; PRG), Lotus corniculatus (birds foot trefoil; BFT) and Trifolium pratense (red clover; RC). We show using 16S rDNA ion torrent sequencing that plant epiphytic populations present pre-incubation (0 h) were substantially different to those attached post incubations in the presence of rumen fluid on all forages. Thereafter primary and secondary colonisation events were evident as defined by changes in abundances of attached bacteria and changes in plant chemistry, as assessed using Fourier Tansfrom-Infra Red (FT-IR) spectroscopy. For PRG colonisation, primary colonisation was seen up to 4 h and secondary colonisation from 4 h onwards. These changes from primary to secondary colonisation occurred significantly later with BFT and RC, with primary colonisation being up to 6 h and secondary colonisation post 6 h of incubation. Across all 3 forages the main colonising bacteria present at all time points post-incubation were Prevotella, Pseudobutyrivibrio, Ruminococcus, Olsenella, Butyrivibrio and Anaeroplasma (14.2, 5.4, 1.9, 2.7, 1.8, 2.0% on average respectively) with Pseudobutyrivibrio and Anaeroplasma having a higher abundance during secondary colonisation. Using CowPI we predict that there were differences between bacterial metabolic function during primary and secondary colonisation. Specifically, carbohydrate metabolism were predicted to be increased in the bacteria attached during secondary colonisation, irrespective of forage type. The CowPI data coupled with the FT-IR plant chemistry data suggest that attached bacterial function is similar irrespective of forage type, with the main changes occurring between primary and secondary colonisation. These data suggest that the sward composition of pasture may have major implications for the temporal availability of nutrients for animal.

Keywords

  • microbiome, perennial ryegrass, birds foot trefoil, red clover, 16S rRNA gene, FTIR, CowPI, rumen