Using next-generation sequencing to determine diversity of horse intestinal worms: identifying the equine ‘nemabiome’

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Using next-generation sequencing to determine diversity of horse intestinal worms: identifying the equine ‘nemabiome’. / Mitchell, Catriona; O'Sullivan, Catherine; Pinloche, Eric Jean-Yves Roger; Wilkinson, Toby; Morphew, Russ; McEwan, Neil.

In: Journal of Equine Science, Vol. 30, No. 1, 14.02.2019, p. 1-5.

Research output: Contribution to journalArticle

Harvard

Mitchell, C, O'Sullivan, C, Pinloche, EJ-YR, Wilkinson, T, Morphew, R & McEwan, N 2019, 'Using next-generation sequencing to determine diversity of horse intestinal worms: identifying the equine ‘nemabiome’' Journal of Equine Science, vol. 30, no. 1, pp. 1-5. https://doi.org/10.1294/jes.30.1

APA

Mitchell, C., O'Sullivan, C., Pinloche, E. J-Y. R., Wilkinson, T., Morphew, R., & McEwan, N. (2019). Using next-generation sequencing to determine diversity of horse intestinal worms: identifying the equine ‘nemabiome’. Journal of Equine Science, 30(1), 1-5. https://doi.org/10.1294/jes.30.1

Vancouver

Mitchell C, O'Sullivan C, Pinloche EJ-YR, Wilkinson T, Morphew R, McEwan N. Using next-generation sequencing to determine diversity of horse intestinal worms: identifying the equine ‘nemabiome’. Journal of Equine Science. 2019 Feb 14;30(1):1-5. https://doi.org/10.1294/jes.30.1

Author

Mitchell, Catriona ; O'Sullivan, Catherine ; Pinloche, Eric Jean-Yves Roger ; Wilkinson, Toby ; Morphew, Russ ; McEwan, Neil. / Using next-generation sequencing to determine diversity of horse intestinal worms: identifying the equine ‘nemabiome’. In: Journal of Equine Science. 2019 ; Vol. 30, No. 1. pp. 1-5.

Bibtex - Download

@article{fdd9a24d7fae48188741141990570461,
title = "Using next-generation sequencing to determine diversity of horse intestinal worms: identifying the equine ‘nemabiome’",
abstract = "Next-generation sequencing of DNA from nematode eggs has been utilised to give the first account of the equine ‘nemabiome’. In all equine faecal samples investigated, multiple species of Strongylidae were detected, ranging from 7.5 (SEM 0.79) with 99+{\%} identity to sequences in the NCBI database to 13.3 (SEM 0.80) with 90+{\%} identity. This range is typical of the number of species described previously in morphological studies using large quantities of digesta per animal. However, the current method is non-invasive; relies on DNA analysis, avoiding the need for specialist microscopy identification; and can be carried out with small samples, providing significant advantages over current methods.",
keywords = "Diversity, Faecal Egg, horse, Nemabiome",
author = "Catriona Mitchell and Catherine O'Sullivan and Pinloche, {Eric Jean-Yves Roger} and Toby Wilkinson and Russ Morphew and Neil McEwan",
year = "2019",
month = "2",
day = "14",
doi = "10.1294/jes.30.1",
language = "English",
volume = "30",
pages = "1--5",
journal = "Journal of Equine Science",
issn = "1340-3516",
publisher = "Japanese Society of Equine Science",
number = "1",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Using next-generation sequencing to determine diversity of horse intestinal worms: identifying the equine ‘nemabiome’

AU - Mitchell, Catriona

AU - O'Sullivan, Catherine

AU - Pinloche, Eric Jean-Yves Roger

AU - Wilkinson, Toby

AU - Morphew, Russ

AU - McEwan, Neil

PY - 2019/2/14

Y1 - 2019/2/14

N2 - Next-generation sequencing of DNA from nematode eggs has been utilised to give the first account of the equine ‘nemabiome’. In all equine faecal samples investigated, multiple species of Strongylidae were detected, ranging from 7.5 (SEM 0.79) with 99+% identity to sequences in the NCBI database to 13.3 (SEM 0.80) with 90+% identity. This range is typical of the number of species described previously in morphological studies using large quantities of digesta per animal. However, the current method is non-invasive; relies on DNA analysis, avoiding the need for specialist microscopy identification; and can be carried out with small samples, providing significant advantages over current methods.

AB - Next-generation sequencing of DNA from nematode eggs has been utilised to give the first account of the equine ‘nemabiome’. In all equine faecal samples investigated, multiple species of Strongylidae were detected, ranging from 7.5 (SEM 0.79) with 99+% identity to sequences in the NCBI database to 13.3 (SEM 0.80) with 90+% identity. This range is typical of the number of species described previously in morphological studies using large quantities of digesta per animal. However, the current method is non-invasive; relies on DNA analysis, avoiding the need for specialist microscopy identification; and can be carried out with small samples, providing significant advantages over current methods.

KW - Diversity

KW - Faecal Egg

KW - horse

KW - Nemabiome

U2 - 10.1294/jes.30.1

DO - 10.1294/jes.30.1

M3 - Article

VL - 30

SP - 1

EP - 5

JO - Journal of Equine Science

JF - Journal of Equine Science

SN - 1340-3516

IS - 1

ER -

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