Transcriptional and Metabolomic Analyses Indicate that Cell Wall Properties are Associated with Drought Tolerance in Brachypodium distachyon

Ingo Lenk; Lorraine Fisher; Martin Vickers; Aderemi Akinyemi; Thomas Didion; Martin Swain; Christian Sig Jensen; Luis Mur; Maurice Bosch

doi:10.3390/ijms20071758

Transcriptional and Metabolomic Analyses Indicate that Cell Wall Properties are Associated with Drought Tolerance in Brachypodium distachyon

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Transcriptional and Metabolomic Analyses Indicate that Cell Wall Properties are Associated with Drought Tolerance in Brachypodium distachyon. / Lenk, Ingo; Fisher, Lorraine; Vickers, Martin et al.

In: International Journal of Molecular Sciences, Vol. 20, No. 7, 1758, 10.04.2019.

Research output: Contribution to journal › Article › peer-review

Harvard

Lenk, I, Fisher, L, Vickers, M, Akinyemi, A, Didion, T, Swain, M, Sig Jensen, C, Mur, L & Bosch, M 2019, 'Transcriptional and Metabolomic Analyses Indicate that Cell Wall Properties are Associated with Drought Tolerance in Brachypodium distachyon', International Journal of Molecular Sciences, vol. 20, no. 7, 1758. https://doi.org/10.3390/ijms20071758

APA

Lenk, I., Fisher, L., Vickers, M., Akinyemi, A., Didion, T., Swain, M., Sig Jensen, C., Mur, L., & Bosch, M. (2019). Transcriptional and Metabolomic Analyses Indicate that Cell Wall Properties are Associated with Drought Tolerance in Brachypodium distachyon. International Journal of Molecular Sciences, 20(7), [1758]. https://doi.org/10.3390/ijms20071758

Vancouver

Lenk I, Fisher L, Vickers M, Akinyemi A, Didion T, Swain M et al. Transcriptional and Metabolomic Analyses Indicate that Cell Wall Properties are Associated with Drought Tolerance in Brachypodium distachyon. International Journal of Molecular Sciences. 2019 Apr 10;20(7):1758. doi: 10.3390/ijms20071758

Author

Lenk, Ingo ; Fisher, Lorraine ; Vickers, Martin et al. / Transcriptional and Metabolomic Analyses Indicate that Cell Wall Properties are Associated with Drought Tolerance in Brachypodium distachyon. In: International Journal of Molecular Sciences. 2019 ; Vol. 20, No. 7.

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@article{a253da283d2d486d9f11609a5de27f4c,

title = "Transcriptional and Metabolomic Analyses Indicate that Cell Wall Properties are Associated with Drought Tolerance in Brachypodium distachyon",

abstract = "Brachypodium distachyon is an established model for drought tolerance. We previously identified accessions exhibiting high tolerance, susceptibility and intermediate tolerance to drought; respectively, ABR8, KOZ1 and ABR4. Transcriptomics and metabolomic approaches were used to define tolerance mechanisms. Transcriptional analyses suggested relatively few drought responsive genes in ABR8 compared to KOZ1. Linking these to gene ontology (GO) terms indicated enrichment for “regulated stress response”, “plant cell wall” and “oxidative stress” associated genes. Further, tolerance correlated with pre-existing differences in cell wall-associated gene expression including glycoside hydrolases, pectin methylesterases, expansins and a pectin acetylesterase. Metabolomic assessments of the same samples also indicated few significant changes in ABR8 with drought. Instead, pre-existing differences in the cell wall-associated metabolites correlated with drought tolerance. Although other features, e.g., jasmonate signaling were suggested in our study, cell wall-focused events appeared to be predominant. Our data suggests two different modes through which the cell wall could confer drought tolerance: (i) An active response mode linked to stress induced changes in cell wall features, and (ii) an intrinsic mode where innate differences in cell wall composition and architecture are important. Both modes seem to contribute to ABR8 drought tolerance. Identification of the exact mechanisms through which the cell wall confers drought tolerance will be important in order to inform development of drought tolerant crops",

keywords = "Brachypodium distachyon, cell wall, drought, FIE-MS, mass spectroscopy, metabolite profiling, RNA-seq, transcriptome profiling",

author = "Ingo Lenk and Lorraine Fisher and Martin Vickers and Aderemi Akinyemi and Thomas Didion and Martin Swain and {Sig Jensen}, Christian and Luis Mur and Maurice Bosch",

year = "2019",

month = apr,

day = "10",

doi = "10.3390/ijms20071758",

language = "English",

volume = "20",

journal = "International Journal of Molecular Sciences",

issn = "1661-6596",

publisher = "Multidisciplinary Digital Publishing Institute",

number = "7",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Transcriptional and Metabolomic Analyses Indicate that Cell Wall Properties are Associated with Drought Tolerance in Brachypodium distachyon

AU - Lenk, Ingo

AU - Fisher, Lorraine

AU - Vickers, Martin

AU - Akinyemi, Aderemi

AU - Didion, Thomas

AU - Swain, Martin

AU - Sig Jensen, Christian

AU - Mur, Luis

AU - Bosch, Maurice

PY - 2019/4/10

Y1 - 2019/4/10

N2 - Brachypodium distachyon is an established model for drought tolerance. We previously identified accessions exhibiting high tolerance, susceptibility and intermediate tolerance to drought; respectively, ABR8, KOZ1 and ABR4. Transcriptomics and metabolomic approaches were used to define tolerance mechanisms. Transcriptional analyses suggested relatively few drought responsive genes in ABR8 compared to KOZ1. Linking these to gene ontology (GO) terms indicated enrichment for “regulated stress response”, “plant cell wall” and “oxidative stress” associated genes. Further, tolerance correlated with pre-existing differences in cell wall-associated gene expression including glycoside hydrolases, pectin methylesterases, expansins and a pectin acetylesterase. Metabolomic assessments of the same samples also indicated few significant changes in ABR8 with drought. Instead, pre-existing differences in the cell wall-associated metabolites correlated with drought tolerance. Although other features, e.g., jasmonate signaling were suggested in our study, cell wall-focused events appeared to be predominant. Our data suggests two different modes through which the cell wall could confer drought tolerance: (i) An active response mode linked to stress induced changes in cell wall features, and (ii) an intrinsic mode where innate differences in cell wall composition and architecture are important. Both modes seem to contribute to ABR8 drought tolerance. Identification of the exact mechanisms through which the cell wall confers drought tolerance will be important in order to inform development of drought tolerant crops

AB - Brachypodium distachyon is an established model for drought tolerance. We previously identified accessions exhibiting high tolerance, susceptibility and intermediate tolerance to drought; respectively, ABR8, KOZ1 and ABR4. Transcriptomics and metabolomic approaches were used to define tolerance mechanisms. Transcriptional analyses suggested relatively few drought responsive genes in ABR8 compared to KOZ1. Linking these to gene ontology (GO) terms indicated enrichment for “regulated stress response”, “plant cell wall” and “oxidative stress” associated genes. Further, tolerance correlated with pre-existing differences in cell wall-associated gene expression including glycoside hydrolases, pectin methylesterases, expansins and a pectin acetylesterase. Metabolomic assessments of the same samples also indicated few significant changes in ABR8 with drought. Instead, pre-existing differences in the cell wall-associated metabolites correlated with drought tolerance. Although other features, e.g., jasmonate signaling were suggested in our study, cell wall-focused events appeared to be predominant. Our data suggests two different modes through which the cell wall could confer drought tolerance: (i) An active response mode linked to stress induced changes in cell wall features, and (ii) an intrinsic mode where innate differences in cell wall composition and architecture are important. Both modes seem to contribute to ABR8 drought tolerance. Identification of the exact mechanisms through which the cell wall confers drought tolerance will be important in order to inform development of drought tolerant crops

KW - Brachypodium distachyon

KW - cell wall

KW - drought

KW - FIE-MS

KW - mass spectroscopy

KW - metabolite profiling

KW - RNA-seq

KW - transcriptome profiling

U2 - 10.3390/ijms20071758

DO - 10.3390/ijms20071758

M3 - Article

C2 - 30974727