Towards defining heterotic gene pools using SSR markers in pearl millet [Pennisetum glaucum (L.) R. Br.]

Authors Organisations
  • Radhika Ramya(Author)
    International Crops Research Institute for the Semi-Arid Tropics
  • Lal Ahmed M(Author)
    International Crops Research Institute for the Semi-Arid Tropics
  • C. Tara Satyavathi(Author)
    Indian Agricultural Research Institute (IARI), New Delhi, India
    All India Co-ordinated Pear Millet Improvement Project (AICPMIP)
  • Abhishek Rathore(Author)
    International Crops Research Institute for the Semi-Arid Tropics
  • Pooja Katiyar(Author)
    International Crops Research Institute for the Semi-Arid Tropics
  • Bhaskar Raj(Author)
    International Crops Research Institute for the Semi-Arid Tropics
  • Sushil Kumar(Author)
    Gujarat Agricultural University
  • Rajeev Gupta(Author)
    International Crops Research Institute for the Semi-Arid Tropics
  • Mahesh Mahendarkar(Author)
    International Crops Research Institute for the Semi-Arid Tropics
  • Rattan Yadav(Author)
  • Rakesh Srivastava(Author)
    International Crops Research Institute for the Semi-Arid Tropics
Type Article
Original languageEnglish
Article number1934
JournalFrontiers in Plant Science
Volume8
DOI
Publication statusPublished - 02 Mar 2018
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Abstract

Pearl millet is a climate resilient crop and the most widely grown millet worldwide. In a maiden attempt to identify potential heterotic groups for grain yield in pearl millet, a total of 88 polymorphic SSR markers were used to genotype 343 hybrid parental lines of pearl millet. The SSR markers generated a total of 532 alleles with a mean value of 6.05 alleles per locus, mean gene
diversity of 0.55, and an average PIC of 0.50. Out of 532 alleles, 443 (83.27%) alleles were contributed by B- lines with a mean of 5.03 alleles per locus. R- lines contributed 476 alleles (89.47%) with a mean of 5.41, while 441 (82.89%) alleles were shared commonly between B- and R- lines. The gene diversity and PIC were high among R- lines (0.55 and 0.50) than B- lines (0.49 and 0.44) revealed that R- lines were more diverse than B- lines. The unweighted neighbor-joining tree based on simple matching dissimilarity matrix obtained from SSR data clearly differentiated B- lines into 10 sub-clusters (B1, B2, B3, B4, B5, B6, B7, B8, B9 and B10), and Rlines into 11 sub-clusters (R1, R2, R3, R4, R5, R6, R7, R8, R9, R10 and R11). The parents, three checks and 99 hybrids generated by crossing between representative lines of each of the B- cluster with that of each of the R- cluster were evaluated in line × tester design over three environments. Based on pooled mean performance, the cross combinations generated between clusters B1 and R3, B2 and R4, B3 and R5, B4 and undetermined cluster, B5 and 11R, B6 and R3, B8 and R4, B9 and R7 and B10 and R5 had shown higher grain yield per plant compared to their counterparts. Based on per se performance, high sca effects and standard heterosis
over superior check, F1s generated from crosses between representatives of groups B3 and B10 with representative of group R5 resulted in best heterotic combinations for grain yield. These represent putative heterotic gene pools in pearl millet.

Keywords

  • pearl millet, B- (maintainer), R- (restorer), SSR (simple sequence repear) markers, gene diversity, PIC (Polymorphic information content), heterotic groups