The Yin-Yang of the Green Fluorescent ProteinImpact on Saccharomyces cerevisiae stress resistance

Authors Organisations
  • Mélanie Ragon(Author)
    Institut Agro
  • Lucie Bertheau(Author)
    Institut Agro
  • Jennifer Dumont(Author)
    Institut Agro
  • Tiffany Bellanger(Author)
    Institut Agro
  • Marie Grosselin(Author)
    Institut Agro
  • Mohini Basu(Author)
    Institut Agro
  • Eléonore Pourcelot(Author)
    Institut Agro
  • Walid Horrigue(Author)
    Institut Agro
  • Emmanuel Denimal(Author)
    Appui à la Recherche
  • Ambroise Marin(Author)
    DImaCell Platform Université de Bourgogne - INRAE
  • Basile Vaucher(Author)
    Institut Agro
  • Antoine Berland(Author)
    Institut Agro
  • Corentin Lepoivre(Author)
    Institut Agro
  • Sébastien Dupont(Author)
    Institut Agro
  • Laurent Beney(Author)
    Institut Agro
  • Hazel Davey(Author)
  • Stéphane Guyot(Author)
    Institut Agro
Type Article
Original languageEnglish
Article number112603
Number of pages12
JournalJournal of Photochemistry and Photobiology B: Biology
Early online date29 Nov 2022
Publication statusPublished - 01 Jan 2023
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Although fluorescent proteins are widely used as biomarkers (Yin), no study focuses on their influence on the microbial stress response. Here, the Green Fluorescent Protein (GFP) was fused to two proteins of interest in Saccharomyces cerevisiae. Pab1p and Sur7p, respectively involved in stress granules structure and in Can1 membrane domains. These were chosen since questions remain regarding the understanding of the behavior of S. cerevisiae facing different heat kinetics or oxidative stresses. The main results showed that Pab1p-GFP fluorescent mutant displayed a higher resistance than that of the wild type under a heat shock. Moreover, fluorescent mutants exposed to oxidative stresses displayed changes in the cultivability compared to the wild type strain. In silico approaches showed that the presence of the GFP did not influence the structure and so the functionality of the tagged proteins meaning that changes in yeast resistance were certainly related to GFP ROS-scavenging ability (Yang).


  • Green fluorescent protein, Heating kinetics, Membrane patches, Oxidation, Saccharomyces cerevisiae, Stress granules