The rumen eukaryotome is a source of novel antimicrobial peptides with therapeutic potential

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The rumen eukaryotome is a source of novel antimicrobial peptides with therapeutic potential. / Onime, Lucy A.; Oyama, Linda B.; Thomas, Benjamin J.; Gani, Jurnorain; Alexander, Peter; Waddams, Kate E.; Cookson, Alan; Fernandez-Fuentes, Narcis; Creevey, Christopher J.; Huws, Sharon A.

In: BMC Microbiology, Vol. 21, No. 1, 105, 08.04.2021.

Research output: Contribution to journalArticlepeer-review

Harvard

Onime, LA, Oyama, LB, Thomas, BJ, Gani, J, Alexander, P, Waddams, KE, Cookson, A, Fernandez-Fuentes, N, Creevey, CJ & Huws, SA 2021, 'The rumen eukaryotome is a source of novel antimicrobial peptides with therapeutic potential', BMC Microbiology, vol. 21, no. 1, 105. https://doi.org/10.1186/s12866-021-02172-8

APA

Onime, L. A., Oyama, L. B., Thomas, B. J., Gani, J., Alexander, P., Waddams, K. E., Cookson, A., Fernandez-Fuentes, N., Creevey, C. J., & Huws, S. A. (2021). The rumen eukaryotome is a source of novel antimicrobial peptides with therapeutic potential. BMC Microbiology, 21(1), [105]. https://doi.org/10.1186/s12866-021-02172-8

Vancouver

Onime LA, Oyama LB, Thomas BJ, Gani J, Alexander P, Waddams KE et al. The rumen eukaryotome is a source of novel antimicrobial peptides with therapeutic potential. BMC Microbiology. 2021 Apr 8;21(1). 105. https://doi.org/10.1186/s12866-021-02172-8

Author

Onime, Lucy A. ; Oyama, Linda B. ; Thomas, Benjamin J. ; Gani, Jurnorain ; Alexander, Peter ; Waddams, Kate E. ; Cookson, Alan ; Fernandez-Fuentes, Narcis ; Creevey, Christopher J. ; Huws, Sharon A. / The rumen eukaryotome is a source of novel antimicrobial peptides with therapeutic potential. In: BMC Microbiology. 2021 ; Vol. 21, No. 1.

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@article{6b4134d0bba34345b291e71da342fcd4,
title = "The rumen eukaryotome is a source of novel antimicrobial peptides with therapeutic potential",
abstract = "Background: The rise of microbial antibiotic resistance is a leading threat to the health of the human population. As such, finding new approaches to tackle these microbes, including development of novel antibiotics is vital. Results: In this study, we mined a rumen eukaryotic metatranscriptomic library for novel Antimicrobial peptides (AMPs) using computational approaches and thereafter characterised the therapeutic potential of the AMPs. We identified a total of 208 potentially novel AMPs from the ruminal eukaryotome, and characterised one of those, namely Lubelisin. Lubelisin (GIVAWFWRLAR) is an α-helical peptide, 11 amino acid long with theoretical molecular weight of 1373.76 D. In the presence of Lubelisin, strains of methicillin-resistant Staphylococcus aureus (MRSA) USA300 and EMRSA-15 were killed within 30 min of exposure with ≥103 and 104 CFU/mL reduction in viable cells respectively. Cytotoxicity of Lubelisin against both human and sheep erythrocytes was low resulting in a therapeutic index of 0.43. Membrane permeabilisation assays using propidium iodide alongside transmission electron microscopy revealed that cytoplasmic membrane damage may contribute to the antimicrobial activities of Lubelisin. Conclusions: We demonstrate that the rumen eukaryotome is a viable source for the discovery of antimicrobial molecules for the treatment of bacterial infections and further development of these may provide part of the potential solution to the ongoing problem of antimicrobial resistance. The role of these AMPs in the ecological warfare within the rumen is also currently unknown.",
keywords = "Antimicrobial peptide, Antimicrobials, Eukaryotes, Eukaryotome, Microbiome, Resistance, Rumen",
author = "Onime, {Lucy A.} and Oyama, {Linda B.} and Thomas, {Benjamin J.} and Jurnorain Gani and Peter Alexander and Waddams, {Kate E.} and Alan Cookson and Narcis Fernandez-Fuentes and Creevey, {Christopher J.} and Huws, {Sharon A.}",
note = "Funding Information: This project was funded by the Life Sciences Research Network Wales. Publisher Copyright: {\textcopyright} 2021, The Author(s).",
year = "2021",
month = apr,
day = "8",
doi = "10.1186/s12866-021-02172-8",
language = "English",
volume = "21",
journal = "BMC Microbiology",
issn = "1471-2180",
publisher = "Springer Nature",
number = "1",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - The rumen eukaryotome is a source of novel antimicrobial peptides with therapeutic potential

AU - Onime, Lucy A.

AU - Oyama, Linda B.

AU - Thomas, Benjamin J.

AU - Gani, Jurnorain

AU - Alexander, Peter

AU - Waddams, Kate E.

AU - Cookson, Alan

AU - Fernandez-Fuentes, Narcis

AU - Creevey, Christopher J.

AU - Huws, Sharon A.

N1 - Funding Information: This project was funded by the Life Sciences Research Network Wales. Publisher Copyright: © 2021, The Author(s).

PY - 2021/4/8

Y1 - 2021/4/8

N2 - Background: The rise of microbial antibiotic resistance is a leading threat to the health of the human population. As such, finding new approaches to tackle these microbes, including development of novel antibiotics is vital. Results: In this study, we mined a rumen eukaryotic metatranscriptomic library for novel Antimicrobial peptides (AMPs) using computational approaches and thereafter characterised the therapeutic potential of the AMPs. We identified a total of 208 potentially novel AMPs from the ruminal eukaryotome, and characterised one of those, namely Lubelisin. Lubelisin (GIVAWFWRLAR) is an α-helical peptide, 11 amino acid long with theoretical molecular weight of 1373.76 D. In the presence of Lubelisin, strains of methicillin-resistant Staphylococcus aureus (MRSA) USA300 and EMRSA-15 were killed within 30 min of exposure with ≥103 and 104 CFU/mL reduction in viable cells respectively. Cytotoxicity of Lubelisin against both human and sheep erythrocytes was low resulting in a therapeutic index of 0.43. Membrane permeabilisation assays using propidium iodide alongside transmission electron microscopy revealed that cytoplasmic membrane damage may contribute to the antimicrobial activities of Lubelisin. Conclusions: We demonstrate that the rumen eukaryotome is a viable source for the discovery of antimicrobial molecules for the treatment of bacterial infections and further development of these may provide part of the potential solution to the ongoing problem of antimicrobial resistance. The role of these AMPs in the ecological warfare within the rumen is also currently unknown.

AB - Background: The rise of microbial antibiotic resistance is a leading threat to the health of the human population. As such, finding new approaches to tackle these microbes, including development of novel antibiotics is vital. Results: In this study, we mined a rumen eukaryotic metatranscriptomic library for novel Antimicrobial peptides (AMPs) using computational approaches and thereafter characterised the therapeutic potential of the AMPs. We identified a total of 208 potentially novel AMPs from the ruminal eukaryotome, and characterised one of those, namely Lubelisin. Lubelisin (GIVAWFWRLAR) is an α-helical peptide, 11 amino acid long with theoretical molecular weight of 1373.76 D. In the presence of Lubelisin, strains of methicillin-resistant Staphylococcus aureus (MRSA) USA300 and EMRSA-15 were killed within 30 min of exposure with ≥103 and 104 CFU/mL reduction in viable cells respectively. Cytotoxicity of Lubelisin against both human and sheep erythrocytes was low resulting in a therapeutic index of 0.43. Membrane permeabilisation assays using propidium iodide alongside transmission electron microscopy revealed that cytoplasmic membrane damage may contribute to the antimicrobial activities of Lubelisin. Conclusions: We demonstrate that the rumen eukaryotome is a viable source for the discovery of antimicrobial molecules for the treatment of bacterial infections and further development of these may provide part of the potential solution to the ongoing problem of antimicrobial resistance. The role of these AMPs in the ecological warfare within the rumen is also currently unknown.

KW - Antimicrobial peptide

KW - Antimicrobials

KW - Eukaryotes

KW - Eukaryotome

KW - Microbiome

KW - Resistance

KW - Rumen

UR - http://www.scopus.com/inward/record.url?scp=85104085630&partnerID=8YFLogxK

U2 - 10.1186/s12866-021-02172-8

DO - 10.1186/s12866-021-02172-8

M3 - Article

C2 - 33832427

AN - SCOPUS:85104085630

VL - 21

JO - BMC Microbiology

JF - BMC Microbiology

SN - 1471-2180

IS - 1

M1 - 105

ER -

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