The MPB83 antigen from Mycobacterium bovis contains O-linked mannose and (1 → 3)-mannobiose moieties

Authors Organisations
  • Stephen L. Michell(Author)
    Animal and Plant Health Agency
    Defence Science and Technology Laboratory
  • Adam O. Whelan(Author)
    Animal and Plant Health Agency
  • Paul R. Wheeler(Author)
    Animal and Plant Health Agency
  • Maria Panico(Author)
    Imperial College London
  • Richard L. Easton(Author)
    Imperial College London
  • A. Tony Etienne(Author)
    Imperial College London
  • Stuart M. Haslam(Author)
    Imperial College London
  • Anne Dell(Author)
    Imperial College London
  • Howard R. Morri(Author)
    Imperial College London
  • Andrew J. Reason(Author)
    Imperial College London
  • Jean Louis Herrmann(Author)
    Hôpital Saint-Louis
  • Douglas B. Young(Author)
    Imperial College London
  • Glyn Hewinson(Author)
    Animal and Plant Health Agency
Type Article
Original languageEnglish
Pages (from-to)16423-16432
Number of pages10
JournalJournal of Biological Chemistry
Volume278
Issue number18
DOI
Publication statusPublished - 02 May 2003
Links
Permanent link
View graph of relations
Citation formats

Abstract

Mycobacterium tuberculosis and Mycobacterium bovis, the causative agents of human and bovine tuberculosis, have been reported to express a range of surface and secreted glycoproteins, although only one of these has been subjected to detailed structural analysis. We describe the use of a genetic system, in conjunction with lectin binding, to characterize the points of attachment of carbohydrate moieties to the polypeptide backbone of a second mycobacterial glycoprotein, antigen MPB83 from M. bovis. Biochemical and structural analysis of the native MPB83 protein and derived peptides demonstrated the presence of 3 mannose units attached to two threonine residues. Mannose residues were joined by a (1 → 3) linkage, in contrast to the (1 → 2) linkage previously observed in antigen MPT32 from M. tuberculosis and the (1 → 2) and (1 → 6) linkages in other mycobacterial glycolipids and polysaccharides. The identification of glycosylated antigens within the M. tuberculosis complex raises the possibility that the carbohydrate moiety of these glycoproteins might be involved in pathogenesis, either by interaction with mannose receptors on host cells, or as targets or modulators of the cell-mediated immune response. Given such a possibility characterization of mycobacterial glycoproteins is a step toward understanding their functional role and elucidating the mechanisms of mycobacterial glycosylation.