The metabolic transition during disease following infection of Arabidopsis thaliana by Pseudomonas syringae pv. tomato.

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The metabolic transition during disease following infection of Arabidopsis thaliana by Pseudomonas syringae pv. tomato. / Ward, Jane L.; Forcat, Silvia; Beckmann, Manfred; Bennett, Mark; Miller, Sonia J.; Baker, John M.; Hawkins, Nathaniel D.; Vermeer, Cornelia Petronella; Lu, Chuan; Lin, Wanchang; Truman, William M.; Beale, Michael H.; Draper, John; Mansfield, John W.; Grant, Murray.

In: Plant Journal, Vol. 63, No. 3, 08.2010, p. 443-457.

Research output: Contribution to journalArticlepeer-review

Harvard

Ward, JL, Forcat, S, Beckmann, M, Bennett, M, Miller, SJ, Baker, JM, Hawkins, ND, Vermeer, CP, Lu, C, Lin, W, Truman, WM, Beale, MH, Draper, J, Mansfield, JW & Grant, M 2010, 'The metabolic transition during disease following infection of Arabidopsis thaliana by Pseudomonas syringae pv. tomato.', Plant Journal, vol. 63, no. 3, pp. 443-457. https://doi.org/10.1111/j.1365-313X.2010.04254.x

APA

Ward, J. L., Forcat, S., Beckmann, M., Bennett, M., Miller, S. J., Baker, J. M., Hawkins, N. D., Vermeer, C. P., Lu, C., Lin, W., Truman, W. M., Beale, M. H., Draper, J., Mansfield, J. W., & Grant, M. (2010). The metabolic transition during disease following infection of Arabidopsis thaliana by Pseudomonas syringae pv. tomato. Plant Journal, 63(3), 443-457. https://doi.org/10.1111/j.1365-313X.2010.04254.x

Author

Ward, Jane L. ; Forcat, Silvia ; Beckmann, Manfred ; Bennett, Mark ; Miller, Sonia J. ; Baker, John M. ; Hawkins, Nathaniel D. ; Vermeer, Cornelia Petronella ; Lu, Chuan ; Lin, Wanchang ; Truman, William M. ; Beale, Michael H. ; Draper, John ; Mansfield, John W. ; Grant, Murray. / The metabolic transition during disease following infection of Arabidopsis thaliana by Pseudomonas syringae pv. tomato. In: Plant Journal. 2010 ; Vol. 63, No. 3. pp. 443-457.

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@article{a7eaaef4462f4441b36ab7978ceae21e,
title = "The metabolic transition during disease following infection of Arabidopsis thaliana by Pseudomonas syringae pv. tomato.",
abstract = "The outcome of bacterial infection in plants is determined by the ability of the pathogen to successfully occupy the apoplastic space and deliver a constellation of effectors that collectively suppress basal and effector-triggered immune responses. In this study, we examined the metabolic changes associated with establishment of disease using analytical techniques that interrogated a range of chemistries. We demonstrated clear differences in the metabolome of Arabidopsis thaliana leaves infected with virulent Pseudomonas syringae within 8 h of infection. In addition to confirmation of changes in phenolic and indolic compounds, we identified rapid alterations in the abundance of amino acids and other nitrogenous compounds, specific classes of glucosinolates, disaccharides, and molecules that influence the prevalence of reactive oxygen species. Our data illustrate that, superimposed on defence suppression, pathogens reconfigure host metabolism to provide the sustenance required to support exponentially growing populations of apoplastically localized bacteria. We performed a detailed baseline study reporting the metabolic dynamics associated with bacterial infection. Moreover, we have integrated these data with the results of transcriptome profiling to distinguish metabolomic pathways that are transcriptionally activated from those that are post-transcriptionally regulated.",
keywords = "metabolomic, Pseudomonas syringae, Arabidopsis thaliana, mass spectrometry, NMR",
author = "Ward, {Jane L.} and Silvia Forcat and Manfred Beckmann and Mark Bennett and Miller, {Sonia J.} and Baker, {John M.} and Hawkins, {Nathaniel D.} and Vermeer, {Cornelia Petronella} and Chuan Lu and Wanchang Lin and Truman, {William M.} and Beale, {Michael H.} and John Draper and Mansfield, {John W.} and Murray Grant",
note = "Ward, J. L., Forcat, S., Beckmann, M., Bennett, M., Miller, S. J., Baker, J. M., Hawkins, N. D., Vermeer, C. P., Lu, C., Lin, W., Truman, W. M., Beale, M. H., Draper, J., Mansfield, J. W., & Grant, M. (2010). The metabolic transition during disease following infection of Arabidopsis thaliana by Pseudomonas syringae pv. tomato.Plant Journal, 63 (3), 443-457 Sponsorship: BBSRC IMPF: 06.94 ",
year = "2010",
month = aug,
doi = "10.1111/j.1365-313X.2010.04254.x",
language = "English",
volume = "63",
pages = "443--457",
journal = "Plant Journal",
issn = "0960-7412",
publisher = "Wiley",
number = "3",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - The metabolic transition during disease following infection of Arabidopsis thaliana by Pseudomonas syringae pv. tomato.

AU - Ward, Jane L.

AU - Forcat, Silvia

AU - Beckmann, Manfred

AU - Bennett, Mark

AU - Miller, Sonia J.

AU - Baker, John M.

AU - Hawkins, Nathaniel D.

AU - Vermeer, Cornelia Petronella

AU - Lu, Chuan

AU - Lin, Wanchang

AU - Truman, William M.

AU - Beale, Michael H.

AU - Draper, John

AU - Mansfield, John W.

AU - Grant, Murray

N1 - Ward, J. L., Forcat, S., Beckmann, M., Bennett, M., Miller, S. J., Baker, J. M., Hawkins, N. D., Vermeer, C. P., Lu, C., Lin, W., Truman, W. M., Beale, M. H., Draper, J., Mansfield, J. W., & Grant, M. (2010). The metabolic transition during disease following infection of Arabidopsis thaliana by Pseudomonas syringae pv. tomato.Plant Journal, 63 (3), 443-457 Sponsorship: BBSRC IMPF: 06.94

PY - 2010/8

Y1 - 2010/8

N2 - The outcome of bacterial infection in plants is determined by the ability of the pathogen to successfully occupy the apoplastic space and deliver a constellation of effectors that collectively suppress basal and effector-triggered immune responses. In this study, we examined the metabolic changes associated with establishment of disease using analytical techniques that interrogated a range of chemistries. We demonstrated clear differences in the metabolome of Arabidopsis thaliana leaves infected with virulent Pseudomonas syringae within 8 h of infection. In addition to confirmation of changes in phenolic and indolic compounds, we identified rapid alterations in the abundance of amino acids and other nitrogenous compounds, specific classes of glucosinolates, disaccharides, and molecules that influence the prevalence of reactive oxygen species. Our data illustrate that, superimposed on defence suppression, pathogens reconfigure host metabolism to provide the sustenance required to support exponentially growing populations of apoplastically localized bacteria. We performed a detailed baseline study reporting the metabolic dynamics associated with bacterial infection. Moreover, we have integrated these data with the results of transcriptome profiling to distinguish metabolomic pathways that are transcriptionally activated from those that are post-transcriptionally regulated.

AB - The outcome of bacterial infection in plants is determined by the ability of the pathogen to successfully occupy the apoplastic space and deliver a constellation of effectors that collectively suppress basal and effector-triggered immune responses. In this study, we examined the metabolic changes associated with establishment of disease using analytical techniques that interrogated a range of chemistries. We demonstrated clear differences in the metabolome of Arabidopsis thaliana leaves infected with virulent Pseudomonas syringae within 8 h of infection. In addition to confirmation of changes in phenolic and indolic compounds, we identified rapid alterations in the abundance of amino acids and other nitrogenous compounds, specific classes of glucosinolates, disaccharides, and molecules that influence the prevalence of reactive oxygen species. Our data illustrate that, superimposed on defence suppression, pathogens reconfigure host metabolism to provide the sustenance required to support exponentially growing populations of apoplastically localized bacteria. We performed a detailed baseline study reporting the metabolic dynamics associated with bacterial infection. Moreover, we have integrated these data with the results of transcriptome profiling to distinguish metabolomic pathways that are transcriptionally activated from those that are post-transcriptionally regulated.

KW - metabolomic

KW - Pseudomonas syringae

KW - Arabidopsis thaliana

KW - mass spectrometry

KW - NMR

U2 - 10.1111/j.1365-313X.2010.04254.x

DO - 10.1111/j.1365-313X.2010.04254.x

M3 - Article

C2 - 20497374

VL - 63

SP - 443

EP - 457

JO - Plant Journal

JF - Plant Journal

SN - 0960-7412

IS - 3

ER -

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