The effect of temperature on the male and female recombination landscape of barley

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The effect of temperature on the male and female recombination landscape of barley. / Phillips, Dylan; Jenkins, Glyn; Macaulay, Malcolm; Nibau, Candida; Wnetrzak, Joanna; Fallding, Derek Stanley; Colas, Isabelle; Oakey, Helena; Waugh, Robbie; Ramsay, Luke.

In: New Phytologist, Vol. 208, No. 2, 10.2015, p. 421-429.

Research output: Contribution to journalArticle

Harvard

Phillips, D, Jenkins, G, Macaulay, M, Nibau, C, Wnetrzak, J, Fallding, DS, Colas, I, Oakey, H, Waugh, R & Ramsay, L 2015, 'The effect of temperature on the male and female recombination landscape of barley', New Phytologist, vol. 208, no. 2, pp. 421-429. https://doi.org/10.1111/nph.13548

APA

Phillips, D., Jenkins, G., Macaulay, M., Nibau, C., Wnetrzak, J., Fallding, D. S., Colas, I., Oakey, H., Waugh, R., & Ramsay, L. (2015). The effect of temperature on the male and female recombination landscape of barley. New Phytologist, 208(2), 421-429. https://doi.org/10.1111/nph.13548

Vancouver

Phillips D, Jenkins G, Macaulay M, Nibau C, Wnetrzak J, Fallding DS et al. The effect of temperature on the male and female recombination landscape of barley. New Phytologist. 2015 Oct;208(2):421-429. https://doi.org/10.1111/nph.13548

Author

Phillips, Dylan ; Jenkins, Glyn ; Macaulay, Malcolm ; Nibau, Candida ; Wnetrzak, Joanna ; Fallding, Derek Stanley ; Colas, Isabelle ; Oakey, Helena ; Waugh, Robbie ; Ramsay, Luke. / The effect of temperature on the male and female recombination landscape of barley. In: New Phytologist. 2015 ; Vol. 208, No. 2. pp. 421-429.

Bibtex - Download

@article{7d42b7956b4b4f1bb2bf833f6ade6628,
title = "The effect of temperature on the male and female recombination landscape of barley",
abstract = "Barley (Hordeum vulgare) is a crop of global significance. However, a third of the genes of barley are largely inaccessible to conventional breeding programmes as crossovers are localised to the ends of the chromosomes. This work examines whether crossovers can be shifted to more proximal regions simply by elevating growth temperature.We utilised a genome-wide marker set for linkage analysis combined with cytological mapping of crossover events to examine the recombination landscape of plants grown at different temperatures.We found that barley shows heterochiasmy, that is, differences between female and male recombination frequencies. In addition, we found that elevated temperature significantly changes patterns of recombination in male meiosis only, with a repositioning of Class I crossovers determined by cytological mapping of HvMLH3 foci. We show that the length of synaptonemal complexes in male meiocytes increases in response to temperature.The results demonstrate that the distribution of crossover events are malleable and can be shifted to proximal regions by altering the growth temperature. The shift in recombination is the result of altering the distribution of Class I crossovers, but the higher recombination at elevated temperatures is potentially not the result of an increase in Class I events.",
keywords = "barley (Hordeum vulgare), crossover, heterochiasmy, MLH3, recombination, synaptonemal complex",
author = "Dylan Phillips and Glyn Jenkins and Malcolm Macaulay and Candida Nibau and Joanna Wnetrzak and Fallding, {Derek Stanley} and Isabelle Colas and Helena Oakey and Robbie Waugh and Luke Ramsay",
note = "This is the peer reviewed version of the following article: Phillips, D., Jenkins, G., Macaulay, M., Nibau, C., Wnetrzak, J., Fallding, D. S., Colas, I., Oakey, H., Waugh, R., Ramsay, L. (2015). The effect of temperature on the male and female recombination landscape of barley. New Phytologist, 208(2), 421-429, which has been published in final form at http://dx.doi.org/doi:10.1111/nph.13548. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving",
year = "2015",
month = oct,
doi = "10.1111/nph.13548",
language = "English",
volume = "208",
pages = "421--429",
journal = "New Phytologist",
issn = "0028-646X",
publisher = "Wiley",
number = "2",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - The effect of temperature on the male and female recombination landscape of barley

AU - Phillips, Dylan

AU - Jenkins, Glyn

AU - Macaulay, Malcolm

AU - Nibau, Candida

AU - Wnetrzak, Joanna

AU - Fallding, Derek Stanley

AU - Colas, Isabelle

AU - Oakey, Helena

AU - Waugh, Robbie

AU - Ramsay, Luke

N1 - This is the peer reviewed version of the following article: Phillips, D., Jenkins, G., Macaulay, M., Nibau, C., Wnetrzak, J., Fallding, D. S., Colas, I., Oakey, H., Waugh, R., Ramsay, L. (2015). The effect of temperature on the male and female recombination landscape of barley. New Phytologist, 208(2), 421-429, which has been published in final form at http://dx.doi.org/doi:10.1111/nph.13548. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving

PY - 2015/10

Y1 - 2015/10

N2 - Barley (Hordeum vulgare) is a crop of global significance. However, a third of the genes of barley are largely inaccessible to conventional breeding programmes as crossovers are localised to the ends of the chromosomes. This work examines whether crossovers can be shifted to more proximal regions simply by elevating growth temperature.We utilised a genome-wide marker set for linkage analysis combined with cytological mapping of crossover events to examine the recombination landscape of plants grown at different temperatures.We found that barley shows heterochiasmy, that is, differences between female and male recombination frequencies. In addition, we found that elevated temperature significantly changes patterns of recombination in male meiosis only, with a repositioning of Class I crossovers determined by cytological mapping of HvMLH3 foci. We show that the length of synaptonemal complexes in male meiocytes increases in response to temperature.The results demonstrate that the distribution of crossover events are malleable and can be shifted to proximal regions by altering the growth temperature. The shift in recombination is the result of altering the distribution of Class I crossovers, but the higher recombination at elevated temperatures is potentially not the result of an increase in Class I events.

AB - Barley (Hordeum vulgare) is a crop of global significance. However, a third of the genes of barley are largely inaccessible to conventional breeding programmes as crossovers are localised to the ends of the chromosomes. This work examines whether crossovers can be shifted to more proximal regions simply by elevating growth temperature.We utilised a genome-wide marker set for linkage analysis combined with cytological mapping of crossover events to examine the recombination landscape of plants grown at different temperatures.We found that barley shows heterochiasmy, that is, differences between female and male recombination frequencies. In addition, we found that elevated temperature significantly changes patterns of recombination in male meiosis only, with a repositioning of Class I crossovers determined by cytological mapping of HvMLH3 foci. We show that the length of synaptonemal complexes in male meiocytes increases in response to temperature.The results demonstrate that the distribution of crossover events are malleable and can be shifted to proximal regions by altering the growth temperature. The shift in recombination is the result of altering the distribution of Class I crossovers, but the higher recombination at elevated temperatures is potentially not the result of an increase in Class I events.

KW - barley (Hordeum vulgare)

KW - crossover

KW - heterochiasmy

KW - MLH3

KW - recombination

KW - synaptonemal complex

UR - http://hdl.handle.net/2160/36418

U2 - 10.1111/nph.13548

DO - 10.1111/nph.13548

M3 - Article

C2 - 26255865

VL - 208

SP - 421

EP - 429

JO - New Phytologist

JF - New Phytologist

SN - 0028-646X

IS - 2

ER -

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