The effect of resistance to mildew infection on ruminal fermentation of Lolium perenne

Type Conference Proceeding (Non-Journal item)
Original languageEnglish
Title of host publicationEGF at 50: The future of European grasslands. Proceedings of the 25th General Meeting of the European Grassland Federation, Aberystwyth, Wales, 7-11 September 2014.
Subtitle of host publication Grassland Science in Europe
EditorsAlan Hopkins
PublisherPrifysgol Aberystwyth | Aberystwyth University
Number of pages851
ISBN (Print)978-0992694012, 0992694019
Publication statusPublished - Sep 2014
EventProceedings of the 25th General Meeting of the European Grassland Federation - Aberystwyth, United Kingdom of Great Britain and Northern Ireland
Duration: 07 Sep 201411 Sep 2014


ConferenceProceedings of the 25th General Meeting of the European Grassland Federation
Country/TerritoryUnited Kingdom of Great Britain and Northern Ireland
Period07 Sep 201411 Sep 2014
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In the field, resistance to microbial pathogens increases crop yield.
The work described here was designed to investigate whether the priming
of endogenous anti-microbial defence responses associated with
resistance mechanisms could affect subsequent utilization by the rumen
micro-organisms during colonization and fermentation of the ingested
fresh forage. The non-discriminatory approach Fourier Transform
Infra-Red Spectroscopy (FTIR) was used to profile the entire metabolome
generated during in vitro fermentation of Lolium perenne leaves which
had been previously infected with an avirulent mildew, thus generating
localized lesions of plant-cell death as part of the Hypersensitive
Response defence reaction. Regardless of mildew exposure there was an
effect of incubation time on metabolic profile, with separation becoming
more obvious with increasing fermentation time. Within these individual
timepoints, inoculation with mildew was also seen to affect the
metabolic profile with the profiles of uninfected (control) leaves
clustering away from infected leaves at later timepoints. We therefore
propose that pre-exposure of grass to infection by avirulent mildew
elicits plant defence responses that modify subsequent colonization by
the rumen microbial population. The consequences of this for
fermentation efficiency are currently being assessed.