The antigen-specific memory CD8+ T-cell response induced by BCG in cattle resides in the CD8+γ/δTCR-CD45RO+ T-cell population

Authors Organisations
  • Alison E. Hogg(Author)
    Institute of Animal Health
  • Andrew Worth(Author)
    Edward Jenner Institute for Vaccine Research
  • Peter Beverley(Author)
    Edward Jenner Institute for Vaccine Research
  • Christopher J. Howard(Author)
    Institute of Animal Health
  • Bernardo Villarreal-Ramos(Author)
    Institute of Animal Health
Type Article
Original languageEnglish
Pages (from-to)270-279
Number of pages10
JournalVaccine
Volume27
Issue number2
Early online date07 Nov 2008
DOI
Publication statusPublished - 07 Jan 2009
Externally publishedYes
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Abstract

Tuberculosis (TB) remains a worldwide leading cause of death among infectious diseases. Development of safer and more efficacious vaccines requires a basic understanding of the protective mechanisms induced by BCG. Here we show that vaccination of cattle with BCG induces CD8+γ/δTCR-CD45RO+ T-cells that can produce IFN-γ, up-regulate transcription and expression of perforin, lyse BCG-infected monocyte-derived macrophages (MoMφ{symbol}) and contribute to a reduction in the number of intracellular mycobacteria. We also observed BCG-induced CD8+ responses in vivo. After infection of cattle with Mycobacterium bovis, CD8+γ/δTCR-CD45RO+ cells responded more strongly to M. bovis-infected MoMφ{symbol} than to BCG-infected MoMφ{symbol}. These results indicate that the antigen-specific CD8+ memory response resides in the CD8+γ/δTCR-CD45RO+ cell population.

Keywords

  • CD8 T-cells, Memory, Tuberculosis