Smell of Infectiona novel, non-invasive method for detection of fish excretory- secretory proteins

Standard

Smell of Infection : a novel, non-invasive method for detection of fish excretory- secretory proteins. / Pawluk, Rebecca; Stuart, Rebekah; Garcia de Leaniz, Carlos; Cable, Jo; Morphew, Russ; Brophy, Peter; Consuegra, Sofia.

In: Journal of Proteome Research, Vol. 18, No. 3, 01.03.2019, p. 1371-1379.

Research output: Contribution to journalArticle

Harvard

Pawluk, R, Stuart, R, Garcia de Leaniz, C, Cable, J, Morphew, R, Brophy, P & Consuegra, S 2019, 'Smell of Infection: a novel, non-invasive method for detection of fish excretory- secretory proteins' Journal of Proteome Research, vol. 18, no. 3, pp. 1371-1379. https://doi.org/10.1021/acs.jproteome.8b00953

APA

Pawluk, R., Stuart, R., Garcia de Leaniz, C., Cable, J., Morphew, R., Brophy, P., & Consuegra, S. (2019). Smell of Infection: a novel, non-invasive method for detection of fish excretory- secretory proteins. Journal of Proteome Research, 18(3), 1371-1379. https://doi.org/10.1021/acs.jproteome.8b00953

Vancouver

Pawluk R, Stuart R, Garcia de Leaniz C, Cable J, Morphew R, Brophy P et al. Smell of Infection: a novel, non-invasive method for detection of fish excretory- secretory proteins. Journal of Proteome Research. 2019 Mar 1;18(3):1371-1379. https://doi.org/10.1021/acs.jproteome.8b00953

Author

Pawluk, Rebecca ; Stuart, Rebekah ; Garcia de Leaniz, Carlos ; Cable, Jo ; Morphew, Russ ; Brophy, Peter ; Consuegra, Sofia. / Smell of Infection : a novel, non-invasive method for detection of fish excretory- secretory proteins. In: Journal of Proteome Research. 2019 ; Vol. 18, No. 3. pp. 1371-1379.

Bibtex - Download

@article{187f791623024eefb6bf0c0da0e396ee,
title = "Smell of Infection: a novel, non-invasive method for detection of fish excretory- secretory proteins",
abstract = "Chemical signals are produced by aquatic organisms following predatory attacks or perturbations such as parasitic infection. Ectoparasites feeding on fish hosts are likely to cause release of similar alarm cues into the environment due to the stress, wounding and immune response stimulated upon infection. Alarm cues are often released in the form of proteins and peptides and provide important insights into bodily function and infection status. Here we outline a non-invasive method to identify potential chemical cues associated with infection in fish by extracting, purifying and characterizing proteins from water samples from cultured fish. Gel free proteomic methods were deemed the most suitable for protein detection in saline water samples. It was confirmed that teleost proteins can be characterised from water samples and that variation in protein profiles could be detected between infected and uninfected individuals and fish and parasite only water samples. Our novel assay provides a non-invasive method for assessing the health condition of both wild and farmed aquatic organisms. Similar to environmental DNA monitoring methods, these proteomic techniques could provide an important tool in applied ecology and aquaculture biology.",
keywords = "alarm cues, gel free MS, Kryptolebias marmoratus, odour, parasitic infection",
author = "Rebecca Pawluk and Rebekah Stuart and {Garcia de Leaniz}, Carlos and Jo Cable and Russ Morphew and Peter Brophy and Sofia Consuegra",
year = "2019",
month = "3",
day = "1",
doi = "10.1021/acs.jproteome.8b00953",
language = "English",
volume = "18",
pages = "1371--1379",
journal = "Journal of Proteome Research",
issn = "1535-3893",
publisher = "American Chemical Society",
number = "3",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Smell of Infection

T2 - a novel, non-invasive method for detection of fish excretory- secretory proteins

AU - Pawluk, Rebecca

AU - Stuart, Rebekah

AU - Garcia de Leaniz, Carlos

AU - Cable, Jo

AU - Morphew, Russ

AU - Brophy, Peter

AU - Consuegra, Sofia

PY - 2019/3/1

Y1 - 2019/3/1

N2 - Chemical signals are produced by aquatic organisms following predatory attacks or perturbations such as parasitic infection. Ectoparasites feeding on fish hosts are likely to cause release of similar alarm cues into the environment due to the stress, wounding and immune response stimulated upon infection. Alarm cues are often released in the form of proteins and peptides and provide important insights into bodily function and infection status. Here we outline a non-invasive method to identify potential chemical cues associated with infection in fish by extracting, purifying and characterizing proteins from water samples from cultured fish. Gel free proteomic methods were deemed the most suitable for protein detection in saline water samples. It was confirmed that teleost proteins can be characterised from water samples and that variation in protein profiles could be detected between infected and uninfected individuals and fish and parasite only water samples. Our novel assay provides a non-invasive method for assessing the health condition of both wild and farmed aquatic organisms. Similar to environmental DNA monitoring methods, these proteomic techniques could provide an important tool in applied ecology and aquaculture biology.

AB - Chemical signals are produced by aquatic organisms following predatory attacks or perturbations such as parasitic infection. Ectoparasites feeding on fish hosts are likely to cause release of similar alarm cues into the environment due to the stress, wounding and immune response stimulated upon infection. Alarm cues are often released in the form of proteins and peptides and provide important insights into bodily function and infection status. Here we outline a non-invasive method to identify potential chemical cues associated with infection in fish by extracting, purifying and characterizing proteins from water samples from cultured fish. Gel free proteomic methods were deemed the most suitable for protein detection in saline water samples. It was confirmed that teleost proteins can be characterised from water samples and that variation in protein profiles could be detected between infected and uninfected individuals and fish and parasite only water samples. Our novel assay provides a non-invasive method for assessing the health condition of both wild and farmed aquatic organisms. Similar to environmental DNA monitoring methods, these proteomic techniques could provide an important tool in applied ecology and aquaculture biology.

KW - alarm cues

KW - gel free MS

KW - Kryptolebias marmoratus

KW - odour

KW - parasitic infection

U2 - 10.1021/acs.jproteome.8b00953

DO - 10.1021/acs.jproteome.8b00953

M3 - Article

VL - 18

SP - 1371

EP - 1379

JO - Journal of Proteome Research

JF - Journal of Proteome Research

SN - 1535-3893

IS - 3

ER -

View graph of relations
Citation formats