Single cell analysis technique for comparison of specific mRNA abundance in plant cells

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Type Article
Original languageEnglish
Pages (from-to)1089-1092
Number of pages4
JournalJournal of Plant Physiology
Volume158
Issue number8
DOI
Publication statusPublished - 2001
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Abstract

A single cell sampling technique for determining changes in gene expression in a range of cell types is described. Expression of actin and changes in expression of a light inducible gene (sucrose: fructan fructosyltransferase) was demonstrated in mesophyll and parenchymatous bundle sheath cells. This method provides a reliable technique for the analysis of specific gene transcript abundance in individual plant cells.

Keywords

  • Hordeum vulgare, Lolium temulentum, micro-sampling, single cell analysis