Secondary metabolite profiling of the model legume Lotus japonicus during its symbiotic interaction with Mesorhizobium loti

Standard

Secondary metabolite profiling of the model legume Lotus japonicus during its symbiotic interaction with Mesorhizobium loti. / Rispail, N.; Hauck, B. D.; Bartholomew, B.; Watson, A. A.; Nash, R. J.; Webb, K. J.

In: Symbiosis, Vol. 50, No. 3, 01.03.2010, p. 119-128.

Research output: Contribution to journalArticle

Bibtex - Download

@article{d806c4a441eb44a48fb76f0c399d2543,
title = "Secondary metabolite profiling of the model legume Lotus japonicus during its symbiotic interaction with Mesorhizobium loti",
abstract = "Plant secondary metabolites, particularly flavonoids, are key components in the early stages of nitrogen-fixing symbiosis. Despite their importance, the endogenous secondary metabolites involved in symbiosis have not yet been identified in the model legume Lotus japonicus. We therefore determined changes in the secondary metabolic profile of Lotus japonicus roots in response to its symbiont. Analysis of the root secondary metabolite profiles 1 week after inoculation with Mesorhizobium loti revealed quantitative changes in the level of 14 phenolic peaks when compared with non-inoculated control plants. These changes affected compounds from most phenolic classes, possibly resulting from interconversion between classes since the total phenolic level remained constant. In addition, the use of 2 M. loti strains differing only in their capacity to synthesise Nod factor revealed that, although Nod factor signalling induced accumulation of a specific subset of 4 phenolic peaks, most changes were induced in response to both rhizobial strains.",
keywords = "Flavonoids, Lotus japonicus, Mesorhizobium loti, Non-nodulating mutants, Nodulation, Nod factor signalling, Secondary metabolites, Symbiosis",
author = "N. Rispail and Hauck, {B. D.} and B. Bartholomew and Watson, {A. A.} and Nash, {R. J.} and Webb, {K. J.}",
note = "Rispail, N., Hauck, B. D., Bartholomew, B., Watson, A. A., Nash, R. J., Webb, K. J. (2010). Secondary metabolite profiling of the model legume Lotus japonicus during its symbiotic interaction with Mesorhizobium loti. Symbiosis, 50 (3), 119-128. IMPF: 01.44 RONO: BCO 3003;CB 03134 ",
year = "2010",
month = mar,
day = "1",
doi = "10.1007/s13199-010-0053-7",
language = "English",
volume = "50",
pages = "119--128",
journal = "Symbiosis",
issn = "0334-5114",
publisher = "Springer Nature",
number = "3",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Secondary metabolite profiling of the model legume Lotus japonicus during its symbiotic interaction with Mesorhizobium loti

AU - Rispail, N.

AU - Hauck, B. D.

AU - Bartholomew, B.

AU - Watson, A. A.

AU - Nash, R. J.

AU - Webb, K. J.

N1 - Rispail, N., Hauck, B. D., Bartholomew, B., Watson, A. A., Nash, R. J., Webb, K. J. (2010). Secondary metabolite profiling of the model legume Lotus japonicus during its symbiotic interaction with Mesorhizobium loti. Symbiosis, 50 (3), 119-128. IMPF: 01.44 RONO: BCO 3003;CB 03134

PY - 2010/3/1

Y1 - 2010/3/1

N2 - Plant secondary metabolites, particularly flavonoids, are key components in the early stages of nitrogen-fixing symbiosis. Despite their importance, the endogenous secondary metabolites involved in symbiosis have not yet been identified in the model legume Lotus japonicus. We therefore determined changes in the secondary metabolic profile of Lotus japonicus roots in response to its symbiont. Analysis of the root secondary metabolite profiles 1 week after inoculation with Mesorhizobium loti revealed quantitative changes in the level of 14 phenolic peaks when compared with non-inoculated control plants. These changes affected compounds from most phenolic classes, possibly resulting from interconversion between classes since the total phenolic level remained constant. In addition, the use of 2 M. loti strains differing only in their capacity to synthesise Nod factor revealed that, although Nod factor signalling induced accumulation of a specific subset of 4 phenolic peaks, most changes were induced in response to both rhizobial strains.

AB - Plant secondary metabolites, particularly flavonoids, are key components in the early stages of nitrogen-fixing symbiosis. Despite their importance, the endogenous secondary metabolites involved in symbiosis have not yet been identified in the model legume Lotus japonicus. We therefore determined changes in the secondary metabolic profile of Lotus japonicus roots in response to its symbiont. Analysis of the root secondary metabolite profiles 1 week after inoculation with Mesorhizobium loti revealed quantitative changes in the level of 14 phenolic peaks when compared with non-inoculated control plants. These changes affected compounds from most phenolic classes, possibly resulting from interconversion between classes since the total phenolic level remained constant. In addition, the use of 2 M. loti strains differing only in their capacity to synthesise Nod factor revealed that, although Nod factor signalling induced accumulation of a specific subset of 4 phenolic peaks, most changes were induced in response to both rhizobial strains.

KW - Flavonoids

KW - Lotus japonicus

KW - Mesorhizobium loti

KW - Non-nodulating mutants

KW - Nodulation

KW - Nod factor signalling

KW - Secondary metabolites

KW - Symbiosis

U2 - 10.1007/s13199-010-0053-7

DO - 10.1007/s13199-010-0053-7

M3 - Article

VL - 50

SP - 119

EP - 128

JO - Symbiosis

JF - Symbiosis

SN - 0334-5114

IS - 3

ER -

View graph of relations
Citation formats