Screening for potential co-products in a Miscanthus sinensis mapping family by liquid chromatography with mass spectrometry detection

TY - JOUR

T1 - Screening for potential co-products in a Miscanthus sinensis mapping family by liquid chromatography with mass spectrometry detection

AU - Parveen, Ifat

AU - Wilson, Thomas

AU - Threadgill, Michael D.

AU - Luyten, Jacob

AU - Roberts, Ruth Elen

AU - Robson, Paul Russell

AU - Hauck, Barbara Dorothea

AU - Donnison, Iain Simon

AU - Winters, Anne Louise

N1 - Funding Information: Work reported in this manuscript was funded by the Biotechnology and Biological Sciences Research Council (BBSRC) Institute Strategic Programme Grant on Energy Grasses & Biorefining (BB/J0042/1). We gratefully acknowledge support for BEACON from the European Regional Development Fund through the Welsh European Funding Office, part of the Welsh Assembly. We are also grateful to Sue Youell, Dr. Charlotte Hayes and Jo Spikes for useful discussions.

PY - 2014/9/1

Y1 - 2014/9/1

N2 - Society is demanding more green chemicals from sustainable sources. Miscanthus is a potential source of platform chemicals and bioethanol through fermentation. Miscanthus sinensis (M. sinensis) has been found to contain particularly high levels of soluble phenols (hydroxycinnamates and flavonoids) which may have application in the nutraceutical, cosmetic and pharmaceutical industries. Here, we describe the first study on the identification and quantification of phenols from the leaf tissue of a bi-parental M. sinensis mapping family. Parents and progeny showed complex profiles of phenols with highly related structures which complicated characterisation of individual phenotypes. Separation of semi-purified extracts by reverse-phase liquid chromatography, coupled with detection by diode array and ESI-MS/MS, enabled distinction of different profiles of phenols. Ten hydroxycinnamates (O-cinnamoylquinic acids) and several flavones (one mono-O-glycosyl flavone, eight mono-C-glycosyl flavones, two di-C-glycosyl flavones, five O-glycosyl-C-glycosyl flavones and nine 2″-O-glycosyl-C-glycosyl flavones) were identified and quantified in leaf tissue of two hundred progeny and maternal and paternal plants during the seedling stage. Progeny exhibiting high, moderate and low amounts of hydroxycinnamates and flavonoids and both parents were selected and screened at seven months’ growth to determine the abundance of these phenols at their highest biomass and compared with seedlings. Concentrations of phenols generally decreased as leaves matured. Several flavone-glycosides were identified. This technique can be used for rapid screening of plants in a mapping family to identify genotypes with high phenol content to add value in the biorefinery chain. This comparative study provides information on the content of potentially valuable compounds from readily renewable resources and possible biomarkers for identification in breeding programmes.

AB - Society is demanding more green chemicals from sustainable sources. Miscanthus is a potential source of platform chemicals and bioethanol through fermentation. Miscanthus sinensis (M. sinensis) has been found to contain particularly high levels of soluble phenols (hydroxycinnamates and flavonoids) which may have application in the nutraceutical, cosmetic and pharmaceutical industries. Here, we describe the first study on the identification and quantification of phenols from the leaf tissue of a bi-parental M. sinensis mapping family. Parents and progeny showed complex profiles of phenols with highly related structures which complicated characterisation of individual phenotypes. Separation of semi-purified extracts by reverse-phase liquid chromatography, coupled with detection by diode array and ESI-MS/MS, enabled distinction of different profiles of phenols. Ten hydroxycinnamates (O-cinnamoylquinic acids) and several flavones (one mono-O-glycosyl flavone, eight mono-C-glycosyl flavones, two di-C-glycosyl flavones, five O-glycosyl-C-glycosyl flavones and nine 2″-O-glycosyl-C-glycosyl flavones) were identified and quantified in leaf tissue of two hundred progeny and maternal and paternal plants during the seedling stage. Progeny exhibiting high, moderate and low amounts of hydroxycinnamates and flavonoids and both parents were selected and screened at seven months’ growth to determine the abundance of these phenols at their highest biomass and compared with seedlings. Concentrations of phenols generally decreased as leaves matured. Several flavone-glycosides were identified. This technique can be used for rapid screening of plants in a mapping family to identify genotypes with high phenol content to add value in the biorefinery chain. This comparative study provides information on the content of potentially valuable compounds from readily renewable resources and possible biomarkers for identification in breeding programmes.

KW - Miscanthus sinensis

KW - mapping family

KW - hydroxycinnamates

KW - flavonoids

KW - LC-DAD-ESI-MSn

KW - green chemicals

KW - Green chemicals

KW - Flavonoids

KW - Mapping family

KW - Hydroxycinnamates

KW - Poaceae/chemistry

KW - Luteolin/analysis

KW - Glycosides/analysis

KW - Phenols/analysis

KW - Tandem Mass Spectrometry

KW - Spectrometry, Mass, Electrospray Ionization

KW - Plant Leaves/chemistry

KW - Chromatography, Liquid

UR - http://hdl.handle.net/2160/34544

UR - http://www.scopus.com/inward/record.url?scp=84905449004&partnerID=8YFLogxK

U2 - 10.1016/j.phytochem.2014.05.003

DO - 10.1016/j.phytochem.2014.05.003

M3 - Article

C2 - 24894362

VL - 105

SP - 186

EP - 196

JO - Phytochemistry

JF - Phytochemistry

SN - 0031-9422

ER -