S. mansoni Schistosomula Antigens Induce Th1/Proinflammatory Cytokine Responses

Authors Organisations
  • Moses Egesa(Author)
    Uganda Virus Research Institute
    Makerere University College of Health Sciences
  • Lawrence Lubyayi(Author)
    Uganda Virus Research Institute
  • Edridah M. Tukahebwa(Author)
    Vector Control Division, Ministry of Heath, Uganda
  • Bernard S. Bagaya(Author)
    Makerere University College of Health Sciences
  • Iain Chalmers(Author)
  • Shona Wilson(Author)
    University of Cambridge
  • Cornelis H. Hokke(Author)
    Leiden University Medical Center
  • Karl Hoffmann(Author)
  • David W. Dunne(Author)
    University of Cambridge
  • Maria Yazdanbakhsh(Author)
    Leiden University Medical Center
  • Lucja A. Labuda(Author)
    Leiden University Medical Center
  • Stephen Cose(Author)
    London School of Hygiene and Tropical Medicine
    Uganda Virus Research Institute
Type Article
Original languageEnglish
Article numbere12592
JournalParasite Immunology
DOI
Publication statusPublished - 21 Sep 2018
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Abstract

Larvae of Schistosoma (schistosomula) are highly susceptible to host immune responses and are attractive prophylactic vaccine targets, although cellular immune responses against schistosomula antigens in endemic human populations are not well characterized. We collected blood and stool from 54 Schistosoma mansoni-infected Ugandans, isolated peripheral blood mononuclear cells and stimulated them for 24 hours with schistosome adult worm and soluble egg antigens (AWA and SEA), along with schistosomula recombinant proteins rSmKK7, Lymphocyte Antigen 6 isoforms (rSmLy6A and rSmLy6B), tetraspanin isoforms (rSmTSP6 and rSmTSP7). Cytokines, chemokines and growth factors were measured in the culture supernatants using a multiplex luminex assay, and infection intensity was determined before and at one year after praziquantel (PZQ) treatment using the Kato Katz method. Cellular responses were grouped and the relationship between groups of correlated cellular responses and infection intensity before and after PZQ treatment investigated. AWA and SEA induced mainly Th2 responses. In contrast, rSmLy6B, rSmTSP6 and rSmTSP7 induced Th1/pro-inflammatory responses. While recombinant antigens rSmKK7 and rSmLy6A did not induce a Th1/pro-inflammatory response, they had an association with pre-treatment infection intensity after adjusting for age and sex. Testing more schistosomula antigens using this approach could provide immune-epidemiology identifiers necessary for prioritizing next generation schistosomiasis vaccine candidates.

Keywords

  • Schistosoma mansoni, Th1/pro-inflammatory, cytokines, schistosomula, vaccine