Role of outer membrane c-type cytochromes MtrC and OmcA in Shewanella oneidensis MR-1 cell production, accumulation, and detachment during respiration on hematite

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Role of outer membrane c-type cytochromes MtrC and OmcA in Shewanella oneidensis MR-1 cell production, accumulation, and detachment during respiration on hematite. / Mitchell, Andrew Charles; Peterson, L.; Reardon, C. L.; Reed, S. B.; Culley, D. E.; Romine, M. R.; Geesey, Gill G.

In: Geobiology, Vol. 10, No. 4, 07.2012, p. 355-370.

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Mitchell, Andrew Charles ; Peterson, L. ; Reardon, C. L. ; Reed, S. B. ; Culley, D. E. ; Romine, M. R. ; Geesey, Gill G. / Role of outer membrane c-type cytochromes MtrC and OmcA in Shewanella oneidensis MR-1 cell production, accumulation, and detachment during respiration on hematite. In: Geobiology. 2012 ; Vol. 10, No. 4. pp. 355-370.

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@article{10718e0861ad436285e44d92f841328e,
title = "Role of outer membrane c-type cytochromes MtrC and OmcA in Shewanella oneidensis MR-1 cell production, accumulation, and detachment during respiration on hematite",
abstract = "The iron-reducing bacterium Shewanella oneidensis MR-1 has the capacity to contribute to iron cycling over the long term by respiring on crystalline iron oxides such as hematite when poorly crystalline phases are depleted. The ability of outer membrane cytochromes OmcA and MtrC of MR-1 to bind to and transfer electrons to hematite has led to the suggestion that they function as terminal reductases when this mineral is used as a respiratory substrate. Differences in their redox behavior and hematite-binding properties, however, indicate that they play different roles in the electron transfer reaction. Here, we investigated how these differences in cytochrome behavior with respect to hematite affected biofilm development when the mineral served as terminal electron acceptor (TEA). Upon attachment to hematite, cells of the wild-type (WT) strain as well as those of a DomcA mutant but not those of a DmtrC mutant replicated and accumulated on the mineral surface. The results indicate that MtrC but not OmcA is required for growth when this mineral serves as TEA. While an OmcA deficiency did not impede cell replication and accumulation on hematite prior to achievement of a maximum surface cell density comparable to that established by WT cells, OmcA was required for efficient electron transfer and cell attachment to hematite once maximum surface cell density was achieved. OmcA may therefore play a role in overcoming barriers to electron transfer and cell attachment to hematite imposed by reductive dissolution of themineral surface fromcell respiration associated with achievement of high surface cell densities.",
author = "Mitchell, {Andrew Charles} and L. Peterson and Reardon, {C. L.} and Reed, {S. B.} and Culley, {D. E.} and Romine, {M. R.} and Geesey, {Gill G.}",
note = "Mitchell, A. C., Peterson, L., Reardon, C. L., Reed, S. B., Culley, D. E., Romine, M. R., Geesey, G. G. (2012). Role of outer membrane c-type cytochromes MtrC and OmcA in Shewanella oneidensis MR-1 cell production, accumulation, and detachment during respiration on hematite. Geobiology, 10 (4), 355-370",
year = "2012",
month = jul,
doi = "10.1111/j.1472-4669.2012.00321.x",
language = "English",
volume = "10",
pages = "355--370",
journal = "Geobiology",
issn = "1472-4677",
publisher = "Wiley",
number = "4",

}

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TY - JOUR

T1 - Role of outer membrane c-type cytochromes MtrC and OmcA in Shewanella oneidensis MR-1 cell production, accumulation, and detachment during respiration on hematite

AU - Mitchell, Andrew Charles

AU - Peterson, L.

AU - Reardon, C. L.

AU - Reed, S. B.

AU - Culley, D. E.

AU - Romine, M. R.

AU - Geesey, Gill G.

N1 - Mitchell, A. C., Peterson, L., Reardon, C. L., Reed, S. B., Culley, D. E., Romine, M. R., Geesey, G. G. (2012). Role of outer membrane c-type cytochromes MtrC and OmcA in Shewanella oneidensis MR-1 cell production, accumulation, and detachment during respiration on hematite. Geobiology, 10 (4), 355-370

PY - 2012/7

Y1 - 2012/7

N2 - The iron-reducing bacterium Shewanella oneidensis MR-1 has the capacity to contribute to iron cycling over the long term by respiring on crystalline iron oxides such as hematite when poorly crystalline phases are depleted. The ability of outer membrane cytochromes OmcA and MtrC of MR-1 to bind to and transfer electrons to hematite has led to the suggestion that they function as terminal reductases when this mineral is used as a respiratory substrate. Differences in their redox behavior and hematite-binding properties, however, indicate that they play different roles in the electron transfer reaction. Here, we investigated how these differences in cytochrome behavior with respect to hematite affected biofilm development when the mineral served as terminal electron acceptor (TEA). Upon attachment to hematite, cells of the wild-type (WT) strain as well as those of a DomcA mutant but not those of a DmtrC mutant replicated and accumulated on the mineral surface. The results indicate that MtrC but not OmcA is required for growth when this mineral serves as TEA. While an OmcA deficiency did not impede cell replication and accumulation on hematite prior to achievement of a maximum surface cell density comparable to that established by WT cells, OmcA was required for efficient electron transfer and cell attachment to hematite once maximum surface cell density was achieved. OmcA may therefore play a role in overcoming barriers to electron transfer and cell attachment to hematite imposed by reductive dissolution of themineral surface fromcell respiration associated with achievement of high surface cell densities.

AB - The iron-reducing bacterium Shewanella oneidensis MR-1 has the capacity to contribute to iron cycling over the long term by respiring on crystalline iron oxides such as hematite when poorly crystalline phases are depleted. The ability of outer membrane cytochromes OmcA and MtrC of MR-1 to bind to and transfer electrons to hematite has led to the suggestion that they function as terminal reductases when this mineral is used as a respiratory substrate. Differences in their redox behavior and hematite-binding properties, however, indicate that they play different roles in the electron transfer reaction. Here, we investigated how these differences in cytochrome behavior with respect to hematite affected biofilm development when the mineral served as terminal electron acceptor (TEA). Upon attachment to hematite, cells of the wild-type (WT) strain as well as those of a DomcA mutant but not those of a DmtrC mutant replicated and accumulated on the mineral surface. The results indicate that MtrC but not OmcA is required for growth when this mineral serves as TEA. While an OmcA deficiency did not impede cell replication and accumulation on hematite prior to achievement of a maximum surface cell density comparable to that established by WT cells, OmcA was required for efficient electron transfer and cell attachment to hematite once maximum surface cell density was achieved. OmcA may therefore play a role in overcoming barriers to electron transfer and cell attachment to hematite imposed by reductive dissolution of themineral surface fromcell respiration associated with achievement of high surface cell densities.

UR - http://hdl.handle.net/2160/13210

U2 - 10.1111/j.1472-4669.2012.00321.x

DO - 10.1111/j.1472-4669.2012.00321.x

M3 - Article

VL - 10

SP - 355

EP - 370

JO - Geobiology

JF - Geobiology

SN - 1472-4677

IS - 4

ER -

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