Recovery of 2R.2Sk Triticale-Aegilops kotschyi Robertsonian Chromosome Translocations

Authors Organisations
  • Waldemar Ulaszewski(Author)
    Polish Academy of Sciences
  • Jolanta Belter(Author)
    Polish Academy of Sciences
  • Halina Wiśniewska (Author)
    Polish Academy of Sciences
  • Joanna Szymczak(Author)
    Polish Academy of Sciences
  • Roksana Skowrońska (Author)
    Polish Academy of Sciences
  • Dylan Phillips(Author)
  • Michal T. Kwiatek(Author)
    Polish Academy of Sciences
Type Article
Original languageEnglish
Article number646
JournalAgronomy
Volume9
Issue number10
DOI
Publication statusPublished - 17 Oct 2019
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Abstract

Robertsonian translocations (RobTs) in the progeny of triticale (×Triticosecale Wittmack) plants with monosomic substitution of Aegilops kotschyi chromosome 2Sk (2R) were investigated by fluorescence in-situ hybridization. Chromosome 2Sk of Ae. kotschyi is reported to possess many valuable loci, such as Lr54 + Yr37 leaf and stripe (yellow) rust resistance genes. We used a standard procedure to produce RobTs, which consisted of self-pollination of monosomic triticale plants, carrying 2R and 2Sk chromosomes in monosomic condition. This approach did not result in RobTs. Simultaneously, we succeeded in producing 11 plants carrying 2R.2Sk compensatory RobTs using an alternative approach that utilized ditelosomic lines of triticale carrying 2RS (short arm) and 2RL (long arm) telosomic chromosomes. Identification of molecular markers linked to Lr54 + Yr37 genes in the translocation plants confirmed that these resources can be exploited in current triticale breeding programmes

Keywords

  • aegilops, centric breaks, chromosome fusion, Robertsonian translocations, telosomic chromosomes, triticale