Quantification of ruminal Clostridium proteoclasticum by real-time PCR using a molecular beacon approach

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Type Article
Original languageEnglish
Pages (from-to)1251-1261
Number of pages11
JournalJournal of Applied Microbiology
Volume103
Issue number4
Early online date17 Apr 2007
DOI
Publication statusPublished - Oct 2007
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Abstract

All members of the ruminal Butyrivibrio group convert linoleic acid (cis-9,cis-12-18:2) via conjugated 18:2 metabolites (mainly cis-9,trans-11-18:2, conjugated linoleic acid) to vaccenic acid (trans-11-18:1), but only members of a small branch, which includes Clostridium proteoclasticum, of this heterogeneous group further reduce vaccenic acid to stearic acid (18:0, SA). The aims of this study were to develop a real-time polymerase chain reaction (PCR) assay that would detect and quantify these key SA producers and to use this method to detect diet-associated changes in their populations in ruminal digesta of lactating cows.

Keywords

  • Animal Nutritional Physiological Phenomena, Animals, Bacterial Typing Techniques, Butyrivibrio, Cattle, Clostridium, DNA, Bacterial, Diet, Female, Gastrointestinal Contents, Polymerase Chain Reaction, RNA, Bacterial, RNA, Ribosomal, 16S, Rumen, Stearic Acids