Proteomic analysis of glutathione transferases from the liver fluke parasite, Fasciola hepatica

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Proteomic analysis of glutathione transferases from the liver fluke parasite, Fasciola hepatica. / Chemale, Gustavo; Morphew, Russell M.; Moxon, Joseph V.; Morassuti, Alessandra L.; LaCourse, E. James; Barrett, John; Johnston, David A.; Brophy, Peter Michael.

In: Proteomics, Vol. 6, No. 23, 23.12.2006, p. 6263-6273.

Research output: Contribution to journalArticle

Harvard

Chemale, G, Morphew, RM, Moxon, JV, Morassuti, AL, LaCourse, EJ, Barrett, J, Johnston, DA & Brophy, PM 2006, 'Proteomic analysis of glutathione transferases from the liver fluke parasite, Fasciola hepatica' Proteomics, vol. 6, no. 23, pp. 6263-6273. https://doi.org/10.1002/pmic.200600499

APA

Chemale, G., Morphew, R. M., Moxon, J. V., Morassuti, A. L., LaCourse, E. J., Barrett, J., ... Brophy, P. M. (2006). Proteomic analysis of glutathione transferases from the liver fluke parasite, Fasciola hepatica. Proteomics, 6(23), 6263-6273. https://doi.org/10.1002/pmic.200600499

Vancouver

Chemale G, Morphew RM, Moxon JV, Morassuti AL, LaCourse EJ, Barrett J et al. Proteomic analysis of glutathione transferases from the liver fluke parasite, Fasciola hepatica. Proteomics. 2006 Dec 23;6(23):6263-6273. https://doi.org/10.1002/pmic.200600499

Author

Chemale, Gustavo ; Morphew, Russell M. ; Moxon, Joseph V. ; Morassuti, Alessandra L. ; LaCourse, E. James ; Barrett, John ; Johnston, David A. ; Brophy, Peter Michael. / Proteomic analysis of glutathione transferases from the liver fluke parasite, Fasciola hepatica. In: Proteomics. 2006 ; Vol. 6, No. 23. pp. 6263-6273.

Bibtex - Download

@article{bbe35939ff484e2fbe440a194ada408f,
title = "Proteomic analysis of glutathione transferases from the liver fluke parasite, Fasciola hepatica",
abstract = "The parasite Fasciola hepatica causes major global disease of livestock, with increasing reports of human infection. Vaccine candidates with varying protection rates have been identified by pre-genomic approaches. As many candidates are part of protein superfamilies, sub-proteomics offers new possibilities to systematically reveal the relative importance of individual family proteins to vaccine formulations within populations. The superfamily glutathione transferase (GST) from liver fluke has phase II detoxification and housekeeping roles, and has been shown to contain protective vaccine candidates. GST were purified from cytosolic fractions of adult flukes using glutathione- and S-hexylglutathione-agarose, separated by 2-DE, and identified by MS/MS, with the support of a liver fluke EST database. All previously described F. hepatica GST isoforms were identified in 2-DE. Amongst the isoforms mapped by 2-DE, a new GST, closely related to the Sigma class enzymes is described for the first time in the liver fluke. We also describe cDNA encoding putative Omega class GST in F. hepatica.",
keywords = "2-DE, F. hepatica, Glutathione transferases, Omega, Sigma",
author = "Gustavo Chemale and Morphew, {Russell M.} and Moxon, {Joseph V.} and Morassuti, {Alessandra L.} and LaCourse, {E. James} and John Barrett and Johnston, {David A.} and Brophy, {Peter Michael}",
note = "Chemale, G., Morphew, R. M., Moxon, J. V., Morassuti, A. L., LaCourse, E. J., Barrett, J., Johnston, D. A., Brophy, P. M. (2006). Proteomic analysis of glutathione transferases from the liver fluke parasite, Fasciola hepatica. Proteomics, 6 (23), 6263-6273 Sponsorship: BBSRC RONO: BB/C503638/1 EU (STREP DELIVER);BBSRC UK; Grant Number: BB/C503638/1: EU(STREP DELIVER) RAE2008",
year = "2006",
month = "12",
day = "23",
doi = "10.1002/pmic.200600499",
language = "English",
volume = "6",
pages = "6263--6273",
journal = "Proteomics",
issn = "1615-9853",
publisher = "Wiley",
number = "23",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Proteomic analysis of glutathione transferases from the liver fluke parasite, Fasciola hepatica

AU - Chemale, Gustavo

AU - Morphew, Russell M.

AU - Moxon, Joseph V.

AU - Morassuti, Alessandra L.

AU - LaCourse, E. James

AU - Barrett, John

AU - Johnston, David A.

AU - Brophy, Peter Michael

N1 - Chemale, G., Morphew, R. M., Moxon, J. V., Morassuti, A. L., LaCourse, E. J., Barrett, J., Johnston, D. A., Brophy, P. M. (2006). Proteomic analysis of glutathione transferases from the liver fluke parasite, Fasciola hepatica. Proteomics, 6 (23), 6263-6273 Sponsorship: BBSRC RONO: BB/C503638/1 EU (STREP DELIVER);BBSRC UK; Grant Number: BB/C503638/1: EU(STREP DELIVER) RAE2008

PY - 2006/12/23

Y1 - 2006/12/23

N2 - The parasite Fasciola hepatica causes major global disease of livestock, with increasing reports of human infection. Vaccine candidates with varying protection rates have been identified by pre-genomic approaches. As many candidates are part of protein superfamilies, sub-proteomics offers new possibilities to systematically reveal the relative importance of individual family proteins to vaccine formulations within populations. The superfamily glutathione transferase (GST) from liver fluke has phase II detoxification and housekeeping roles, and has been shown to contain protective vaccine candidates. GST were purified from cytosolic fractions of adult flukes using glutathione- and S-hexylglutathione-agarose, separated by 2-DE, and identified by MS/MS, with the support of a liver fluke EST database. All previously described F. hepatica GST isoforms were identified in 2-DE. Amongst the isoforms mapped by 2-DE, a new GST, closely related to the Sigma class enzymes is described for the first time in the liver fluke. We also describe cDNA encoding putative Omega class GST in F. hepatica.

AB - The parasite Fasciola hepatica causes major global disease of livestock, with increasing reports of human infection. Vaccine candidates with varying protection rates have been identified by pre-genomic approaches. As many candidates are part of protein superfamilies, sub-proteomics offers new possibilities to systematically reveal the relative importance of individual family proteins to vaccine formulations within populations. The superfamily glutathione transferase (GST) from liver fluke has phase II detoxification and housekeeping roles, and has been shown to contain protective vaccine candidates. GST were purified from cytosolic fractions of adult flukes using glutathione- and S-hexylglutathione-agarose, separated by 2-DE, and identified by MS/MS, with the support of a liver fluke EST database. All previously described F. hepatica GST isoforms were identified in 2-DE. Amongst the isoforms mapped by 2-DE, a new GST, closely related to the Sigma class enzymes is described for the first time in the liver fluke. We also describe cDNA encoding putative Omega class GST in F. hepatica.

KW - 2-DE

KW - F. hepatica

KW - Glutathione transferases

KW - Omega

KW - Sigma

U2 - 10.1002/pmic.200600499

DO - 10.1002/pmic.200600499

M3 - Article

VL - 6

SP - 6263

EP - 6273

JO - Proteomics

JF - Proteomics

SN - 1615-9853

IS - 23

ER -

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