Proteolysis During Ensilage of Forages Varying in Soluble Sugar Content

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Proteolysis During Ensilage of Forages Varying in Soluble Sugar Content. / Davies, D. R.; Merry, R. J.; Williams, A. P.; Bakewell, Eleanor Lydia; Leemans, David; Tweed, John.

In: Journal of Dairy Science, Vol. 81, No. 2, 02.1998, p. 444-453.

Research output: Contribution to journalArticle

Harvard

Davies, DR, Merry, RJ, Williams, AP, Bakewell, EL, Leemans, D & Tweed, J 1998, 'Proteolysis During Ensilage of Forages Varying in Soluble Sugar Content', Journal of Dairy Science, vol. 81, no. 2, pp. 444-453. https://doi.org/10.3168/jds.S0022-0302(98)75596-1

APA

Davies, D. R., Merry, R. J., Williams, A. P., Bakewell, E. L., Leemans, D., & Tweed, J. (1998). Proteolysis During Ensilage of Forages Varying in Soluble Sugar Content. Journal of Dairy Science, 81(2), 444-453. https://doi.org/10.3168/jds.S0022-0302(98)75596-1

Vancouver

Davies DR, Merry RJ, Williams AP, Bakewell EL, Leemans D, Tweed J. Proteolysis During Ensilage of Forages Varying in Soluble Sugar Content. Journal of Dairy Science. 1998 Feb;81(2):444-453. https://doi.org/10.3168/jds.S0022-0302(98)75596-1

Author

Davies, D. R. ; Merry, R. J. ; Williams, A. P. ; Bakewell, Eleanor Lydia ; Leemans, David ; Tweed, John. / Proteolysis During Ensilage of Forages Varying in Soluble Sugar Content. In: Journal of Dairy Science. 1998 ; Vol. 81, No. 2. pp. 444-453.

Bibtex - Download

@article{bea5ecc018ef4cca8cbb34493606fd1e,
title = "Proteolysis During Ensilage of Forages Varying in Soluble Sugar Content",
abstract = "The effect of contrasting concentrations of water-soluble carbohydrates of herbage on silage fermentation and composition was examined using grass with high [250 g/kg of dry matter (DM)] concentrations of water-soluble carbohydrates and grass and clover with low (66 g/kg of DM) concentrations of water-soluble carbohydrates. Herbages were ensiled untreated, after inoculation with lactic acid bacteria, or after treatment with formic acid. Good quality silages were produced from herbage with high concentrations of water-soluble carbohydrates, regardless of treatment, and all pH values were below 3.7 after 90 d of ensilage. However, the silage formed from inoculated herbage had a significantly lower concentration of ammonia N and a significantly higher proportion of residual ribulose-1,5-bisphosphate carboxylase compared with the other two silages. Fast protein liquid chromatography{\textregistered} (Pharmacia, Uppsala, Sweden) was used to measure ribulose-1,5-bisphosphate carboxylase, and measurement of true plant protein fractions in herbage and silage showed benefits over traditional measurements such as the measurement of N and ammonia N. Herbages with low concentrations of water-soluble carbohydrates produced inferior quality silages that had lower ribulose-1,5- bisphosphate carboxylase contents and higher ammonia N contents, regardless of treatment; few significant differences were observed among treatments. Under good ensiling conditions, when available water-soluble carbohydrate is adequate, the use of inoculants can improve fermentation characteristics and increase the ribulose-1,5-bisphosphate carboxylase content of silages. However, when the herbage has low concentrations of water-soluble carbohydrates, even in inoculated herbages, lactic acid bacteria may follow a heterofermentative pathway instead of a homofermentative pathway, which can result in a decrease in silage quality and a reduction in intact ribulose-1,5-bisphosphate carboxylase.",
keywords = "silage, proteolysis, ribulose-1,5-bisphosphate carboxylase, soluble sugar",
author = "Davies, {D. R.} and Merry, {R. J.} and Williams, {A. P.} and Bakewell, {Eleanor Lydia} and David Leemans and John Tweed",
year = "1998",
month = feb,
doi = "10.3168/jds.S0022-0302(98)75596-1",
language = "English",
volume = "81",
pages = "444--453",
journal = "Journal of Dairy Science",
issn = "0022-0302",
publisher = "Elsevier",
number = "2",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Proteolysis During Ensilage of Forages Varying in Soluble Sugar Content

AU - Davies, D. R.

AU - Merry, R. J.

AU - Williams, A. P.

AU - Bakewell, Eleanor Lydia

AU - Leemans, David

AU - Tweed, John

PY - 1998/2

Y1 - 1998/2

N2 - The effect of contrasting concentrations of water-soluble carbohydrates of herbage on silage fermentation and composition was examined using grass with high [250 g/kg of dry matter (DM)] concentrations of water-soluble carbohydrates and grass and clover with low (66 g/kg of DM) concentrations of water-soluble carbohydrates. Herbages were ensiled untreated, after inoculation with lactic acid bacteria, or after treatment with formic acid. Good quality silages were produced from herbage with high concentrations of water-soluble carbohydrates, regardless of treatment, and all pH values were below 3.7 after 90 d of ensilage. However, the silage formed from inoculated herbage had a significantly lower concentration of ammonia N and a significantly higher proportion of residual ribulose-1,5-bisphosphate carboxylase compared with the other two silages. Fast protein liquid chromatography® (Pharmacia, Uppsala, Sweden) was used to measure ribulose-1,5-bisphosphate carboxylase, and measurement of true plant protein fractions in herbage and silage showed benefits over traditional measurements such as the measurement of N and ammonia N. Herbages with low concentrations of water-soluble carbohydrates produced inferior quality silages that had lower ribulose-1,5- bisphosphate carboxylase contents and higher ammonia N contents, regardless of treatment; few significant differences were observed among treatments. Under good ensiling conditions, when available water-soluble carbohydrate is adequate, the use of inoculants can improve fermentation characteristics and increase the ribulose-1,5-bisphosphate carboxylase content of silages. However, when the herbage has low concentrations of water-soluble carbohydrates, even in inoculated herbages, lactic acid bacteria may follow a heterofermentative pathway instead of a homofermentative pathway, which can result in a decrease in silage quality and a reduction in intact ribulose-1,5-bisphosphate carboxylase.

AB - The effect of contrasting concentrations of water-soluble carbohydrates of herbage on silage fermentation and composition was examined using grass with high [250 g/kg of dry matter (DM)] concentrations of water-soluble carbohydrates and grass and clover with low (66 g/kg of DM) concentrations of water-soluble carbohydrates. Herbages were ensiled untreated, after inoculation with lactic acid bacteria, or after treatment with formic acid. Good quality silages were produced from herbage with high concentrations of water-soluble carbohydrates, regardless of treatment, and all pH values were below 3.7 after 90 d of ensilage. However, the silage formed from inoculated herbage had a significantly lower concentration of ammonia N and a significantly higher proportion of residual ribulose-1,5-bisphosphate carboxylase compared with the other two silages. Fast protein liquid chromatography® (Pharmacia, Uppsala, Sweden) was used to measure ribulose-1,5-bisphosphate carboxylase, and measurement of true plant protein fractions in herbage and silage showed benefits over traditional measurements such as the measurement of N and ammonia N. Herbages with low concentrations of water-soluble carbohydrates produced inferior quality silages that had lower ribulose-1,5- bisphosphate carboxylase contents and higher ammonia N contents, regardless of treatment; few significant differences were observed among treatments. Under good ensiling conditions, when available water-soluble carbohydrate is adequate, the use of inoculants can improve fermentation characteristics and increase the ribulose-1,5-bisphosphate carboxylase content of silages. However, when the herbage has low concentrations of water-soluble carbohydrates, even in inoculated herbages, lactic acid bacteria may follow a heterofermentative pathway instead of a homofermentative pathway, which can result in a decrease in silage quality and a reduction in intact ribulose-1,5-bisphosphate carboxylase.

KW - silage

KW - proteolysis

KW - ribulose-1,5-bisphosphate carboxylase

KW - soluble sugar

UR - http://hdl.handle.net/2160/43186

U2 - 10.3168/jds.S0022-0302(98)75596-1

DO - 10.3168/jds.S0022-0302(98)75596-1

M3 - Article

VL - 81

SP - 444

EP - 453

JO - Journal of Dairy Science

JF - Journal of Dairy Science

SN - 0022-0302

IS - 2

ER -

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