Primary and secondary metabolism, and post-translational protein modifications, as portrayed by proteomic analysis of Streptomyces coelicolor

Standard

Primary and secondary metabolism, and post-translational protein modifications, as portrayed by proteomic analysis of Streptomyces coelicolor. / Hesketh, A. R.; Chandra, G.; Shaw, Angharad; Kell, Douglas B.; Rowland, Jeremy John; Bibb, M. J.; Chater, K. F.

In: Molecular Microbiology, Vol. 46, No. 4, 11.2002, p. 917-932.

Research output: Contribution to journalArticle

Author

Hesketh, A. R. ; Chandra, G. ; Shaw, Angharad ; Kell, Douglas B. ; Rowland, Jeremy John ; Bibb, M. J. ; Chater, K. F. / Primary and secondary metabolism, and post-translational protein modifications, as portrayed by proteomic analysis of Streptomyces coelicolor. In: Molecular Microbiology. 2002 ; Vol. 46, No. 4. pp. 917-932.

Bibtex - Download

@article{eab0954826b24177944d9651a94c542a,
title = "Primary and secondary metabolism, and post-translational protein modifications, as portrayed by proteomic analysis of Streptomyces coelicolor",
abstract = "The newly sequenced genome of Streptomyces coelicolor is estimated to encode 7825 theoretical proteins. We have mapped approximately 10% of the theoretical proteome experimentally using two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. Products from 770 different genes were identified, and the types of proteins represented are discussed in terms of their anno-tated functional classes. An average of 1.2 proteins per gene was observed, indicating extensive post-translational regulation. Examples of modification by N-acetylation, adenylylation and proteolytic processing were characterized using mass spectrometry. Proteins from both primary and certain secondary metabolic pathways are strongly represented on the map, and a number of these enzymes were identified at more than one two-dimensional gel location. Post-translational modification mechanisms may therefore play a significant role in the regulation of these pathways. Unexpectedly, one of the enzymes for synthesis of the actinorhodin polyketide antibiotic appears to be located outside the cytoplasmic compartment, within the cell wall matrix. Of 20 gene clusters encoding enzymes characteristic of secondary metabolism, eight are represented on the proteome map, including three that specify the production of novel metabolites. This information will be valuable in the characterization of the new metabolites.",
author = "Hesketh, {A. R.} and G. Chandra and Angharad Shaw and Kell, {Douglas B.} and Rowland, {Jeremy John} and Bibb, {M. J.} and Chater, {K. F.}",
note = "Hesketh, A. R., Chandra, G., Shaw, A. D., Rowland, J. J., Kell, D. B., Bibb, M. J., Chater, K. F. (2002). Primary and secondary metabolism, and post-translational protein modifications, as portrayed by proteomic analysis of Streptomyces coelicolor.   Molecular Microbiology, 46, (4), 917-932 Sponsorship: BBSRC (2/FGT11406, 208/ FGT11408 and 208/IGF12432)",
year = "2002",
month = nov,
doi = "10.1046/j.1365-2958.2002.03219.x",
language = "English",
volume = "46",
pages = "917--932",
journal = "Molecular Microbiology",
issn = "0950-382X",
publisher = "Wiley",
number = "4",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Primary and secondary metabolism, and post-translational protein modifications, as portrayed by proteomic analysis of Streptomyces coelicolor

AU - Hesketh, A. R.

AU - Chandra, G.

AU - Shaw, Angharad

AU - Kell, Douglas B.

AU - Rowland, Jeremy John

AU - Bibb, M. J.

AU - Chater, K. F.

N1 - Hesketh, A. R., Chandra, G., Shaw, A. D., Rowland, J. J., Kell, D. B., Bibb, M. J., Chater, K. F. (2002). Primary and secondary metabolism, and post-translational protein modifications, as portrayed by proteomic analysis of Streptomyces coelicolor.   Molecular Microbiology, 46, (4), 917-932 Sponsorship: BBSRC (2/FGT11406, 208/ FGT11408 and 208/IGF12432)

PY - 2002/11

Y1 - 2002/11

N2 - The newly sequenced genome of Streptomyces coelicolor is estimated to encode 7825 theoretical proteins. We have mapped approximately 10% of the theoretical proteome experimentally using two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. Products from 770 different genes were identified, and the types of proteins represented are discussed in terms of their anno-tated functional classes. An average of 1.2 proteins per gene was observed, indicating extensive post-translational regulation. Examples of modification by N-acetylation, adenylylation and proteolytic processing were characterized using mass spectrometry. Proteins from both primary and certain secondary metabolic pathways are strongly represented on the map, and a number of these enzymes were identified at more than one two-dimensional gel location. Post-translational modification mechanisms may therefore play a significant role in the regulation of these pathways. Unexpectedly, one of the enzymes for synthesis of the actinorhodin polyketide antibiotic appears to be located outside the cytoplasmic compartment, within the cell wall matrix. Of 20 gene clusters encoding enzymes characteristic of secondary metabolism, eight are represented on the proteome map, including three that specify the production of novel metabolites. This information will be valuable in the characterization of the new metabolites.

AB - The newly sequenced genome of Streptomyces coelicolor is estimated to encode 7825 theoretical proteins. We have mapped approximately 10% of the theoretical proteome experimentally using two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. Products from 770 different genes were identified, and the types of proteins represented are discussed in terms of their anno-tated functional classes. An average of 1.2 proteins per gene was observed, indicating extensive post-translational regulation. Examples of modification by N-acetylation, adenylylation and proteolytic processing were characterized using mass spectrometry. Proteins from both primary and certain secondary metabolic pathways are strongly represented on the map, and a number of these enzymes were identified at more than one two-dimensional gel location. Post-translational modification mechanisms may therefore play a significant role in the regulation of these pathways. Unexpectedly, one of the enzymes for synthesis of the actinorhodin polyketide antibiotic appears to be located outside the cytoplasmic compartment, within the cell wall matrix. Of 20 gene clusters encoding enzymes characteristic of secondary metabolism, eight are represented on the proteome map, including three that specify the production of novel metabolites. This information will be valuable in the characterization of the new metabolites.

U2 - 10.1046/j.1365-2958.2002.03219.x

DO - 10.1046/j.1365-2958.2002.03219.x

M3 - Article

VL - 46

SP - 917

EP - 932

JO - Molecular Microbiology

JF - Molecular Microbiology

SN - 0950-382X

IS - 4

ER -

View graph of relations
Citation formats