Pilot-scale production of xylo-oligosaccharides and fermentable sugars from Miscanthus using steam explosion pretreatment

Rakesh Bhatia; Ana Winters; David Bryant; Maurice Bosch; John Clifton-Brown; David  Leak; Joe Gallagher

doi:10.1016/j.biortech.2019.122285

Pilot-scale production of xylo-oligosaccharides and fermentable sugars from Miscanthus using steam explosion pretreatment

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Pilot-scale production of xylo-oligosaccharides and fermentable sugars from Miscanthus using steam explosion pretreatment. / Bhatia, Rakesh; Winters, Ana ; Bryant, David et al.

In: Bioresource Technology, Vol. 296, 122285, 31.01.2020.

Research output: Contribution to journal › Article › peer-review

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@article{d2389fc708d741d2953a3fc82154ad77,

title = "Pilot-scale production of xylo-oligosaccharides and fermentable sugars from Miscanthus using steam explosion pretreatment",

abstract = "This study investigated pilot-scale production of xylo-oligosaccharides (XOS) and fermentable sugars from Miscanthus using steam explosion (SE) pretreatment. SE conditions (200 °C; 15 bar; 10 min) led to XOS yields up to 52 % (w/w) of initial xylan in the hydrolysate. Liquid chromatography-mass spectrometry demonstrated that the solubilised XOS contained bound acetyl- and hydroxycinnamate residues, physicochemical properties known for high prebiotic effects and anti-oxidant activity in nutraceutical foods. Enzymatic hydrolysis of XOS-rich hydrolysate with commercial endo-xylanases resulted in xylobiose yields of 380 to 500 g/kg of initial xylan in the biomass after only 4 h, equivalent to ∼74 to 90 % conversion of XOS into xylobiose. Fermentable glucose yields from enzymatic hydrolysis of solid residues were 8 to 9-fold higher than for untreated material. In view of an integrated bio-refinery, we demonstrate the potential for efficient utilisation of Miscanthus for the production of renewable sources, including biochemicals and biofuels",

keywords = "miscanthus, steam explosion, xylo-oligosaccharides, biofuels, bio-refining, Xylo-oligosaccharides, Steam explosion, Biorefining, Miscanthus, Biofuels, Weights and Measures, Oligosaccharides, Hydrolysis, Steam, Sugars",

author = "Rakesh Bhatia and Ana Winters and David Bryant and Maurice Bosch and John Clifton-Brown and David Leak and Joe Gallagher",

note = "Funding Information: This work was financed by the Biotechnology and Biological Sciences Research Council (BBSRC) and the S{\~a}o Paulo Research Foundation (FAPESP) ( BB/P017460/1 ). We thank Telma Teixeira Franco who provided insight and expertise that assisted the research. The authors also thank Paul Robson and the Miscanthus breeding team at IBERS Aberystwyth University for the hybrids (funded by BBSRC BB/CSP1730/1, BB/N016149/1, BB/K01711X/1, BBS/E/W/10963A01 as well as the GIANT-LINK project LK0863) and the BEACON Biorefining Centre of Excellence for supporting the work presented in this paper. Publisher Copyright: {\textcopyright} 2019",

year = "2020",

month = jan,

day = "31",

doi = "10.1016/j.biortech.2019.122285",

language = "English",

volume = "296",

journal = "Bioresource Technology",

issn = "0960-8524",

publisher = "Elsevier",

}

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TY - JOUR

T1 - Pilot-scale production of xylo-oligosaccharides and fermentable sugars from Miscanthus using steam explosion pretreatment

AU - Bhatia, Rakesh

AU - Winters, Ana

AU - Bryant, David

AU - Bosch, Maurice

AU - Clifton-Brown, John

AU - Leak, David

AU - Gallagher, Joe

N1 - Funding Information: This work was financed by the Biotechnology and Biological Sciences Research Council (BBSRC) and the São Paulo Research Foundation (FAPESP) ( BB/P017460/1 ). We thank Telma Teixeira Franco who provided insight and expertise that assisted the research. The authors also thank Paul Robson and the Miscanthus breeding team at IBERS Aberystwyth University for the hybrids (funded by BBSRC BB/CSP1730/1, BB/N016149/1, BB/K01711X/1, BBS/E/W/10963A01 as well as the GIANT-LINK project LK0863) and the BEACON Biorefining Centre of Excellence for supporting the work presented in this paper. Publisher Copyright: © 2019

PY - 2020/1/31

Y1 - 2020/1/31

N2 - This study investigated pilot-scale production of xylo-oligosaccharides (XOS) and fermentable sugars from Miscanthus using steam explosion (SE) pretreatment. SE conditions (200 °C; 15 bar; 10 min) led to XOS yields up to 52 % (w/w) of initial xylan in the hydrolysate. Liquid chromatography-mass spectrometry demonstrated that the solubilised XOS contained bound acetyl- and hydroxycinnamate residues, physicochemical properties known for high prebiotic effects and anti-oxidant activity in nutraceutical foods. Enzymatic hydrolysis of XOS-rich hydrolysate with commercial endo-xylanases resulted in xylobiose yields of 380 to 500 g/kg of initial xylan in the biomass after only 4 h, equivalent to ∼74 to 90 % conversion of XOS into xylobiose. Fermentable glucose yields from enzymatic hydrolysis of solid residues were 8 to 9-fold higher than for untreated material. In view of an integrated bio-refinery, we demonstrate the potential for efficient utilisation of Miscanthus for the production of renewable sources, including biochemicals and biofuels

AB - This study investigated pilot-scale production of xylo-oligosaccharides (XOS) and fermentable sugars from Miscanthus using steam explosion (SE) pretreatment. SE conditions (200 °C; 15 bar; 10 min) led to XOS yields up to 52 % (w/w) of initial xylan in the hydrolysate. Liquid chromatography-mass spectrometry demonstrated that the solubilised XOS contained bound acetyl- and hydroxycinnamate residues, physicochemical properties known for high prebiotic effects and anti-oxidant activity in nutraceutical foods. Enzymatic hydrolysis of XOS-rich hydrolysate with commercial endo-xylanases resulted in xylobiose yields of 380 to 500 g/kg of initial xylan in the biomass after only 4 h, equivalent to ∼74 to 90 % conversion of XOS into xylobiose. Fermentable glucose yields from enzymatic hydrolysis of solid residues were 8 to 9-fold higher than for untreated material. In view of an integrated bio-refinery, we demonstrate the potential for efficient utilisation of Miscanthus for the production of renewable sources, including biochemicals and biofuels

KW - miscanthus

KW - steam explosion

KW - xylo-oligosaccharides

KW - biofuels

KW - bio-refining

KW - Xylo-oligosaccharides

KW - Steam explosion

KW - Biorefining

KW - Miscanthus

KW - Biofuels

KW - Weights and Measures

KW - Oligosaccharides