Physical and genetic mapping in the grasses Lolium perenne and Festuca pratensis.

TY - JOUR

T1 - Physical and genetic mapping in the grasses Lolium perenne and Festuca pratensis.

AU - King, Julie

AU - Armstead, Ian Peter

AU - Donnison, Iain Simon

AU - Thomas, Huw M.

AU - Jones, Robert Neil

AU - Kearsey, Mike J.

AU - Roberts, Luned Ann

AU - Thomas, A.

AU - Morgan, W. G.

AU - King, Ian Philip

N1 - King, J., Armstead, I. P., Donnison, I. S., Thomas, H. M., Jones, R. N., Kearsey, M. J., Roberts, L. A., Jones, A., King, I. P. (2002). Physical and genetic mapping in the grasses Lolium perenne and Festuca pratensis.   Genetics, 161, (1), 315-324.

PY - 2002

Y1 - 2002

N2 - A single chromosome of the grass species Festuca pratensis has been introgressed into Lolium perenne to produce a diploid monosomic substitution line (2n 2x 14). In this line recombination occurs throughout the length of the F. pratensis/L. perenne bivalent. The F. pratensis chromosome and recombinants between it and its L. perenne homeologue can be visualized using genomic in situ hybridization (GISH). GISH junctions represent the physical locations of sites of recombination, enabling a range of recombinant chromosomes to be used for physical mapping of the introgressed F. pratensis chromosome. The physical map, in conjunction with a genetic map composed of 104 F. pratensis-specific amplified fragment length polymorphisms (AFLPs), demonstrated: (1) the first large-scale analysis of the physical distribution of AFLPs; (2) variation in the relationship between genetic and physical distance from one part of the F. pratensis chromosome to another (e.g., variation was observed between and within chromosome arms); (3) that nucleolar organizer regions (NORs) and centromeres greatly reduce recombination; (4) that coding sequences are present close to the centromere and NORs in areas of low recombination in plant species with large genomes; and (5) apparent complete synteny between the F. pratensis chromosome and rice chromosome 1.

AB - A single chromosome of the grass species Festuca pratensis has been introgressed into Lolium perenne to produce a diploid monosomic substitution line (2n 2x 14). In this line recombination occurs throughout the length of the F. pratensis/L. perenne bivalent. The F. pratensis chromosome and recombinants between it and its L. perenne homeologue can be visualized using genomic in situ hybridization (GISH). GISH junctions represent the physical locations of sites of recombination, enabling a range of recombinant chromosomes to be used for physical mapping of the introgressed F. pratensis chromosome. The physical map, in conjunction with a genetic map composed of 104 F. pratensis-specific amplified fragment length polymorphisms (AFLPs), demonstrated: (1) the first large-scale analysis of the physical distribution of AFLPs; (2) variation in the relationship between genetic and physical distance from one part of the F. pratensis chromosome to another (e.g., variation was observed between and within chromosome arms); (3) that nucleolar organizer regions (NORs) and centromeres greatly reduce recombination; (4) that coding sequences are present close to the centromere and NORs in areas of low recombination in plant species with large genomes; and (5) apparent complete synteny between the F. pratensis chromosome and rice chromosome 1.

M3 - Article

VL - 161

SP - 315

EP - 324

JO - Genetics

JF - Genetics

SN - 0016-6731

ER -