Nitrite and nitric oxide are important in the adjustment of primary metabolism during the hypersensitive response in tobacco

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Nitrite and nitric oxide are important in the adjustment of primary metabolism during the hypersensitive response in tobacco. / Mur, Luis; Kumari, Aprajita; Brotman, Yariv; Zeier, Jürgen; Mandon, Julian; Cristescu, Simona M.; Harren, Frans; Kaiser, Werner ; Fernie, Alisdair R.; Gupta, Kapuganti Jagadis.

In: Journal of Experimental Botany, Vol. 70, No. 17, 10.04.2019, p. 4571-4582.

Research output: Contribution to journalArticle

Harvard

Mur, L, Kumari, A, Brotman, Y, Zeier, J, Mandon, J, Cristescu, SM, Harren, F, Kaiser, W, Fernie, AR & Gupta, KJ 2019, 'Nitrite and nitric oxide are important in the adjustment of primary metabolism during the hypersensitive response in tobacco', Journal of Experimental Botany, vol. 70, no. 17, pp. 4571-4582. https://doi.org/10.1093/jxb/erz161

APA

Mur, L., Kumari, A., Brotman, Y., Zeier, J., Mandon, J., Cristescu, S. M., Harren, F., Kaiser, W., Fernie, A. R., & Gupta, K. J. (2019). Nitrite and nitric oxide are important in the adjustment of primary metabolism during the hypersensitive response in tobacco. Journal of Experimental Botany, 70(17), 4571-4582. https://doi.org/10.1093/jxb/erz161

Vancouver

Mur L, Kumari A, Brotman Y, Zeier J, Mandon J, Cristescu SM et al. Nitrite and nitric oxide are important in the adjustment of primary metabolism during the hypersensitive response in tobacco. Journal of Experimental Botany. 2019 Apr 10;70(17):4571-4582. https://doi.org/10.1093/jxb/erz161

Author

Mur, Luis ; Kumari, Aprajita ; Brotman, Yariv ; Zeier, Jürgen ; Mandon, Julian ; Cristescu, Simona M. ; Harren, Frans ; Kaiser, Werner ; Fernie, Alisdair R. ; Gupta, Kapuganti Jagadis. / Nitrite and nitric oxide are important in the adjustment of primary metabolism during the hypersensitive response in tobacco. In: Journal of Experimental Botany. 2019 ; Vol. 70, No. 17. pp. 4571-4582.

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@article{5b71b60cde21457f853f0da5a6abeb8e,
title = "Nitrite and nitric oxide are important in the adjustment of primary metabolism during the hypersensitive response in tobacco",
abstract = "Nitrate and ammonia deferentially modulate primary metabolism during the hypersensitive response in tobacco. Tobacco RNAi line with low nitrite reductase (NiRr) levels were used to investigate the roles of nitrite and nitric oxide (NO) in this process. This line accumulates NO2-, with increased NO generation, but allows sufficient reduction to NH4+ to maintain plant viability. Inoculation of the NO3- grown wild type and NiRr plants with the non-host biotrophic pathogen Pseudomonas syringae pv. phaseolicola induced an accumulation of nitrite and NO, together a hypersensitive response (HR) that resulted in decreased bacterial growth, increased electrolyte leakage and enhanced pathogen resistance (PR) gene expression. These responses were higher with increase in NO/ NO2- levels in NiRr plants than WT under NO3- nutrition. In contrast NH4+ grown WT and NiRr plants exhibited compromised resistance. A metabolomic analysis detected 141 metabolites, whose abundance was differentially changed as a result of exposure to P. syringae pv and in response to NO/ NO2- accumulation Of these, 13 were involved in primary metabolism and most were linked to amino acid and energy metabolism. HR-associated changes in metabolism that are often linked with primary nitrate assimilation may therefore be influenced by nitrite and NO production",
keywords = "nitrite, nitrate, nitric oxide, amino acid metabolism, nitrite reductase",
author = "Luis Mur and Aprajita Kumari and Yariv Brotman and J{\"u}rgen Zeier and Julian Mandon and Cristescu, {Simona M.} and Frans Harren and Werner Kaiser and Fernie, {Alisdair R.} and Gupta, {Kapuganti Jagadis}",
year = "2019",
month = apr,
day = "10",
doi = "10.1093/jxb/erz161",
language = "English",
volume = "70",
pages = "4571--4582",
journal = "Journal of Experimental Botany",
issn = "0022-0957",
publisher = "Oxford University Press",
number = "17",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Nitrite and nitric oxide are important in the adjustment of primary metabolism during the hypersensitive response in tobacco

AU - Mur, Luis

AU - Kumari, Aprajita

AU - Brotman, Yariv

AU - Zeier, Jürgen

AU - Mandon, Julian

AU - Cristescu, Simona M.

AU - Harren, Frans

AU - Kaiser, Werner

AU - Fernie, Alisdair R.

AU - Gupta, Kapuganti Jagadis

PY - 2019/4/10

Y1 - 2019/4/10

N2 - Nitrate and ammonia deferentially modulate primary metabolism during the hypersensitive response in tobacco. Tobacco RNAi line with low nitrite reductase (NiRr) levels were used to investigate the roles of nitrite and nitric oxide (NO) in this process. This line accumulates NO2-, with increased NO generation, but allows sufficient reduction to NH4+ to maintain plant viability. Inoculation of the NO3- grown wild type and NiRr plants with the non-host biotrophic pathogen Pseudomonas syringae pv. phaseolicola induced an accumulation of nitrite and NO, together a hypersensitive response (HR) that resulted in decreased bacterial growth, increased electrolyte leakage and enhanced pathogen resistance (PR) gene expression. These responses were higher with increase in NO/ NO2- levels in NiRr plants than WT under NO3- nutrition. In contrast NH4+ grown WT and NiRr plants exhibited compromised resistance. A metabolomic analysis detected 141 metabolites, whose abundance was differentially changed as a result of exposure to P. syringae pv and in response to NO/ NO2- accumulation Of these, 13 were involved in primary metabolism and most were linked to amino acid and energy metabolism. HR-associated changes in metabolism that are often linked with primary nitrate assimilation may therefore be influenced by nitrite and NO production

AB - Nitrate and ammonia deferentially modulate primary metabolism during the hypersensitive response in tobacco. Tobacco RNAi line with low nitrite reductase (NiRr) levels were used to investigate the roles of nitrite and nitric oxide (NO) in this process. This line accumulates NO2-, with increased NO generation, but allows sufficient reduction to NH4+ to maintain plant viability. Inoculation of the NO3- grown wild type and NiRr plants with the non-host biotrophic pathogen Pseudomonas syringae pv. phaseolicola induced an accumulation of nitrite and NO, together a hypersensitive response (HR) that resulted in decreased bacterial growth, increased electrolyte leakage and enhanced pathogen resistance (PR) gene expression. These responses were higher with increase in NO/ NO2- levels in NiRr plants than WT under NO3- nutrition. In contrast NH4+ grown WT and NiRr plants exhibited compromised resistance. A metabolomic analysis detected 141 metabolites, whose abundance was differentially changed as a result of exposure to P. syringae pv and in response to NO/ NO2- accumulation Of these, 13 were involved in primary metabolism and most were linked to amino acid and energy metabolism. HR-associated changes in metabolism that are often linked with primary nitrate assimilation may therefore be influenced by nitrite and NO production

KW - nitrite

KW - nitrate

KW - nitric oxide

KW - amino acid metabolism

KW - nitrite reductase

U2 - 10.1093/jxb/erz161

DO - 10.1093/jxb/erz161

M3 - Article

C2 - 31173640

VL - 70

SP - 4571

EP - 4582

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

SN - 0022-0957

IS - 17

ER -

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