Molecular characterisation of kappa-5, a major antigenic glycoprotein from Schistosoma mansoni eggs

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Molecular characterisation of kappa-5, a major antigenic glycoprotein from Schistosoma mansoni eggs. / Schramm, G.; Hamilton, J. V.; Balog, C. I. A.; Wuhrer, M.; Gronow, A.; Beckmann, S.; Wippersteg, V.; Grevelding, C. G.; Goldmann, T.; Weber, E.; Brattig, N. W.; Deelder, A. M.; Dunne, D. W.; Hokke, C. H.; Haas, H.; Doenhoff, M. J.

In: Molecular and Biochemical Parasitology, Vol. 166, No. 1, 07.2009, p. 4-14.

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Harvard

Schramm, G, Hamilton, JV, Balog, CIA, Wuhrer, M, Gronow, A, Beckmann, S, Wippersteg, V, Grevelding, CG, Goldmann, T, Weber, E, Brattig, NW, Deelder, AM, Dunne, DW, Hokke, CH, Haas, H & Doenhoff, MJ 2009, 'Molecular characterisation of kappa-5, a major antigenic glycoprotein from Schistosoma mansoni eggs', Molecular and Biochemical Parasitology, vol. 166, no. 1, pp. 4-14. https://doi.org/10.1016/j.molbiopara.2009.02.003

APA

Schramm, G., Hamilton, J. V., Balog, C. I. A., Wuhrer, M., Gronow, A., Beckmann, S., Wippersteg, V., Grevelding, C. G., Goldmann, T., Weber, E., Brattig, N. W., Deelder, A. M., Dunne, D. W., Hokke, C. H., Haas, H., & Doenhoff, M. J. (2009). Molecular characterisation of kappa-5, a major antigenic glycoprotein from Schistosoma mansoni eggs. Molecular and Biochemical Parasitology, 166(1), 4-14. https://doi.org/10.1016/j.molbiopara.2009.02.003

Vancouver

Schramm G, Hamilton JV, Balog CIA, Wuhrer M, Gronow A, Beckmann S et al. Molecular characterisation of kappa-5, a major antigenic glycoprotein from Schistosoma mansoni eggs. Molecular and Biochemical Parasitology. 2009 Jul;166(1):4-14. https://doi.org/10.1016/j.molbiopara.2009.02.003

Author

Schramm, G. ; Hamilton, J. V. ; Balog, C. I. A. ; Wuhrer, M. ; Gronow, A. ; Beckmann, S. ; Wippersteg, V. ; Grevelding, C. G. ; Goldmann, T. ; Weber, E. ; Brattig, N. W. ; Deelder, A. M. ; Dunne, D. W. ; Hokke, C. H. ; Haas, H. ; Doenhoff, M. J. / Molecular characterisation of kappa-5, a major antigenic glycoprotein from Schistosoma mansoni eggs. In: Molecular and Biochemical Parasitology. 2009 ; Vol. 166, No. 1. pp. 4-14.

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@article{7c374167888346c7bd483d20be0d7813,
title = "Molecular characterisation of kappa-5, a major antigenic glycoprotein from Schistosoma mansoni eggs",
abstract = "The major immunopathological consequences of infection with Schistosoma mansoni, a T helper type 2 response and granuloma formation leading to fibrotic tissue damage, are caused by the egg stage of the parasite. Three antigens of S. mansoni eggs, termed IPSE/alpha-1, omega-1 and kappa-5, have been found to be the primary targets of the egg-directed antibody response of the host. Here, we report on the isolation, cloning and characterisation of kappa-5. Apart from an uncharacterised mRNA sequence in S. japonicum, no significant similarities of kappa-5 to known sequences from other species were found. In contrast to IPSE/alpha-1 and omega-1, which have been found only in eggs, kappa-5 was present in miracidia as well as in eggs at the mRNA and protein levels. In eggs, isoforms of kappa-5 were observed with both three and four fully occupied N-glycosylation sites, while in miracidia only one isoform with four N-glycans could be detected. Interestingly, in Western blots sera from S. mansoni-infected Africans were reactive against kappa-5 with IgE and IgG isotype antibodies, but against IPSE/alpha-1 and omega-1 only with IgG antibodies. The further characterisation of kappa-5 as one of the three major egg antigens should help to better understand the immunology and immunopathology of schistosomiasis.",
keywords = "Schistosoma mansoni, Kappa-5, Egg antigen, SmEA, Excretory/secretory",
author = "G. Schramm and Hamilton, {J. V.} and Balog, {C. I. A.} and M. Wuhrer and A. Gronow and S. Beckmann and V. Wippersteg and Grevelding, {C. G.} and T. Goldmann and E. Weber and Brattig, {N. W.} and Deelder, {A. M.} and Dunne, {D. W.} and Hokke, {C. H.} and H. Haas and Doenhoff, {M. J.}",
note = "Schramm, G., Hamilton, J. V., Balog, C. I. A., Wuhrer, M., Gronow, A., Wippersteg, V., Beckmann, S., Grevelding, C .G., Goldmann, T., Weber, E., Brattig, N. W., Deelder, A. M., Dunne, D. W., Hokke, C.H., Haas, H., Doenhoff, M. J. (2009). Molecular characterisation of kappa-5, a major antigenic glycoprotein from Schistosoma mansoni eggs. Molecular and Biochemical Parasitology, 166 (1), 4-14. IMPF: 02.94 Sponsorship: Forschungsgemeinschaft (DFG), SFB/TR22-TP A12",
year = "2009",
month = jul,
doi = "10.1016/j.molbiopara.2009.02.003",
language = "English",
volume = "166",
pages = "4--14",
journal = "Molecular and Biochemical Parasitology",
issn = "0166-6851",
publisher = "Elsevier",
number = "1",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Molecular characterisation of kappa-5, a major antigenic glycoprotein from Schistosoma mansoni eggs

AU - Schramm, G.

AU - Hamilton, J. V.

AU - Balog, C. I. A.

AU - Wuhrer, M.

AU - Gronow, A.

AU - Beckmann, S.

AU - Wippersteg, V.

AU - Grevelding, C. G.

AU - Goldmann, T.

AU - Weber, E.

AU - Brattig, N. W.

AU - Deelder, A. M.

AU - Dunne, D. W.

AU - Hokke, C. H.

AU - Haas, H.

AU - Doenhoff, M. J.

N1 - Schramm, G., Hamilton, J. V., Balog, C. I. A., Wuhrer, M., Gronow, A., Wippersteg, V., Beckmann, S., Grevelding, C .G., Goldmann, T., Weber, E., Brattig, N. W., Deelder, A. M., Dunne, D. W., Hokke, C.H., Haas, H., Doenhoff, M. J. (2009). Molecular characterisation of kappa-5, a major antigenic glycoprotein from Schistosoma mansoni eggs. Molecular and Biochemical Parasitology, 166 (1), 4-14. IMPF: 02.94 Sponsorship: Forschungsgemeinschaft (DFG), SFB/TR22-TP A12

PY - 2009/7

Y1 - 2009/7

N2 - The major immunopathological consequences of infection with Schistosoma mansoni, a T helper type 2 response and granuloma formation leading to fibrotic tissue damage, are caused by the egg stage of the parasite. Three antigens of S. mansoni eggs, termed IPSE/alpha-1, omega-1 and kappa-5, have been found to be the primary targets of the egg-directed antibody response of the host. Here, we report on the isolation, cloning and characterisation of kappa-5. Apart from an uncharacterised mRNA sequence in S. japonicum, no significant similarities of kappa-5 to known sequences from other species were found. In contrast to IPSE/alpha-1 and omega-1, which have been found only in eggs, kappa-5 was present in miracidia as well as in eggs at the mRNA and protein levels. In eggs, isoforms of kappa-5 were observed with both three and four fully occupied N-glycosylation sites, while in miracidia only one isoform with four N-glycans could be detected. Interestingly, in Western blots sera from S. mansoni-infected Africans were reactive against kappa-5 with IgE and IgG isotype antibodies, but against IPSE/alpha-1 and omega-1 only with IgG antibodies. The further characterisation of kappa-5 as one of the three major egg antigens should help to better understand the immunology and immunopathology of schistosomiasis.

AB - The major immunopathological consequences of infection with Schistosoma mansoni, a T helper type 2 response and granuloma formation leading to fibrotic tissue damage, are caused by the egg stage of the parasite. Three antigens of S. mansoni eggs, termed IPSE/alpha-1, omega-1 and kappa-5, have been found to be the primary targets of the egg-directed antibody response of the host. Here, we report on the isolation, cloning and characterisation of kappa-5. Apart from an uncharacterised mRNA sequence in S. japonicum, no significant similarities of kappa-5 to known sequences from other species were found. In contrast to IPSE/alpha-1 and omega-1, which have been found only in eggs, kappa-5 was present in miracidia as well as in eggs at the mRNA and protein levels. In eggs, isoforms of kappa-5 were observed with both three and four fully occupied N-glycosylation sites, while in miracidia only one isoform with four N-glycans could be detected. Interestingly, in Western blots sera from S. mansoni-infected Africans were reactive against kappa-5 with IgE and IgG isotype antibodies, but against IPSE/alpha-1 and omega-1 only with IgG antibodies. The further characterisation of kappa-5 as one of the three major egg antigens should help to better understand the immunology and immunopathology of schistosomiasis.

KW - Schistosoma mansoni

KW - Kappa-5

KW - Egg antigen

KW - SmEA

KW - Excretory/secretory

U2 - 10.1016/j.molbiopara.2009.02.003

DO - 10.1016/j.molbiopara.2009.02.003

M3 - Article

C2 - 19428667

VL - 166

SP - 4

EP - 14

JO - Molecular and Biochemical Parasitology

JF - Molecular and Biochemical Parasitology

SN - 0166-6851

IS - 1

ER -

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