Molecular characterisation of kappa-5, a major antigenic glycoprotein from Schistosoma mansoni eggs

Authors Organisations
  • G. Schramm(Author)
  • Joanne Hamilton(Author)
  • C. I. A. Balog(Author)
  • M. Wuhrer(Author)
  • A. Gronow(Author)
  • S. Beckmann(Author)
  • V. Wippersteg(Author)
  • C. G. Grevelding(Author)
  • T. Goldmann(Author)
  • E. Weber(Author)
  • N. W. Brattig(Author)
  • A. M. Deelder(Author)
  • D. W. Dunne(Author)
  • C. H. Hokke(Author)
  • H. Haas(Author)
  • M. J. Doenhoff(Author)
Type Article
Original languageEnglish
Pages (from-to)4-14
Number of pages11
JournalMolecular and Biochemical Parasitology
Issue number1
Publication statusPublished - Jul 2009
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The major immunopathological consequences of infection with Schistosoma mansoni, a T helper type 2 response and granuloma formation leading to fibrotic tissue damage, are caused by the egg stage of the parasite. Three antigens of S. mansoni eggs, termed IPSE/alpha-1, omega-1 and kappa-5, have been found to be the primary targets of the egg-directed antibody response of the host. Here, we report on the isolation, cloning and characterisation of kappa-5. Apart from an uncharacterised mRNA sequence in S. japonicum, no significant similarities of kappa-5 to known sequences from other species were found. In contrast to IPSE/alpha-1 and omega-1, which have been found only in eggs, kappa-5 was present in miracidia as well as in eggs at the mRNA and protein levels. In eggs, isoforms of kappa-5 were observed with both three and four fully occupied N-glycosylation sites, while in miracidia only one isoform with four N-glycans could be detected. Interestingly, in Western blots sera from S. mansoni-infected Africans were reactive against kappa-5 with IgE and IgG isotype antibodies, but against IPSE/alpha-1 and omega-1 only with IgG antibodies. The further characterisation of kappa-5 as one of the three major egg antigens should help to better understand the immunology and immunopathology of schistosomiasis.


  • Schistosoma mansoni, Kappa-5, Egg antigen, SmEA, Excretory/secretory