Metabolic molecular markers of the tidal clock in the marine crustacean Eurydice pulchra

Authors Organisations
  • John Stuart O'Neill(Author)
    MRC Laboratory of Molecular Biology, Cambridge
  • Kate D. Lee(Author)
    University of Leicester
  • Lin Zhang(Author)
    University of Leicester
  • Kevin Feeney(Author)
    MRC Laboratory of Molecular Biology, Cambridge
  • Simon George Webster(Author)
    Prifysgol Bangor | Bangor University
  • James Blades(Author)
    University of Leicester
  • Charalambos Panayiotis Kyriacou(Author)
    University of Leicester
  • Michael Hastings(Author)
    MRC Laboratory of Molecular Biology, Cambridge
  • David Wilcockson(Author)
Type Article
Original languageEnglish
Pages (from-to)R326-R327
Number of pages2
JournalCurrent Biology
Volume25
Issue number8
DOI
Publication statusPublished - 20 Apr 2015
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Abstract

In contrast to the well mapped molecular orchestration of circadian timekeeping in terrestrial organisms, the mechanisms that direct tidal and lunar rhythms in marine species are entirely unknown. Using a combination of biochemical and molecular approaches we have identified a series of metabolic markers of the tidal clock of the intertidal isopod Eurydice pulchra. Specifically, we show that the overoxidation of peroxiredoxin (PRX), a conserved marker of circadian timekeeping in terrestrial eukaryotes [1], follows a circatidal (approximately 12.4 hours) pattern in E. pulchra, in register with the tidal pattern of swimming. In parallel, we show that mitochondrially encoded genes are expressed with a circatidal rhythm. Together, these findings demonstrate that PRX overoxidation rhythms are not intrinsically circadian; rather they appear to resonate with the dominant metabolic cycle of an organism, regardless of its frequency. Moreover, they provide the first molecular leads for dissecting the tidal clockwork.