Mapping the self-incompatibility (S) locus in white clover (Trifolium repens L.)

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Mapping the self-incompatibility (S) locus in white clover (Trifolium repens L.). / Casey, N.; Milbourne, D.; Febrer, M.; Abberton, M.; Jenkins, G.; Barth, S.; Thorogood, D.

2009. 118-118 Poster session presented at 18th Meeting of the Eucarpia Fodder Crops and Amenity Grasses Section, La Rochelle, France.

Research output: Contribution to conferencePoster

Harvard

Casey, N, Milbourne, D, Febrer, M, Abberton, M, Jenkins, G, Barth, S & Thorogood, D 2009, 'Mapping the self-incompatibility (S) locus in white clover (Trifolium repens L.)', 18th Meeting of the Eucarpia Fodder Crops and Amenity Grasses Section, La Rochelle, France, 10 May 2009 - 14 May 2009 pp. 118-118.

APA

Casey, N., Milbourne, D., Febrer, M., Abberton, M., Jenkins, G., Barth, S., & Thorogood, D. (2009). Mapping the self-incompatibility (S) locus in white clover (Trifolium repens L.). 118-118. Poster session presented at 18th Meeting of the Eucarpia Fodder Crops and Amenity Grasses Section, La Rochelle, France.

Vancouver

Casey N, Milbourne D, Febrer M, Abberton M, Jenkins G, Barth S et al. Mapping the self-incompatibility (S) locus in white clover (Trifolium repens L.). 2009. Poster session presented at 18th Meeting of the Eucarpia Fodder Crops and Amenity Grasses Section, La Rochelle, France.

Author

Casey, N. ; Milbourne, D. ; Febrer, M. ; Abberton, M. ; Jenkins, G. ; Barth, S. ; Thorogood, D. / Mapping the self-incompatibility (S) locus in white clover (Trifolium repens L.). Poster session presented at 18th Meeting of the Eucarpia Fodder Crops and Amenity Grasses Section, La Rochelle, France.1 p.

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@conference{1ba732b656074106b90f01799cb453dd,
title = "Mapping the self-incompatibility (S) locus in white clover (Trifolium repens L.)",
abstract = "White clover (Trifolium repens L.) is a member of the Leguminosae flowering plant family and is used as a high quality forage crop capable of fixing atmospheric nitrogen. It is an amphidiploid (2n = 4x = 32) species constructed of two ancestral genomes, T. palescens and T. occidentale. T. repens possesses a self-incompatibility mechanism which prevents self-seed setting. The self-incompatibility reaction is contolled by a single poly-allelic locus expressed gametophytically in the male, where common allele specificity in pollen and stylar expressed genes results in late-acting, pre-fertilisation arrest of pollentube growth (Atwood, 1940). Based on the assumption that both parents were heterozygous for unique S-alleles, ninety four individuals of a bi-parental mapping family were classified into four intra-incompatible groupings from partial diallel crosses and pod-filling assessments six to nine days post-pollination of emasculated florets. Initial analysis of the two parental maps based on AFLP and SSR markers has identified marker associations with the S-locus attributing it to linkage group E2 as classified by Barrett et al (2004). To date, 12, 8, 6 and 16 plants have been assigned the S genotypes S13, S14, S23 and S24 respectively. Further work will place more markers on this linkage group, and polymorphisms of candidate genes/proteins will be compared to the S-locus genotypes of the characterised plants. ReferencesAtwood, S.S. (1940). Genetics of cross-incompatibility among self-incompatible plants of Trifolium repens. Journal of American Society of Agronomy 32: 955-968. Barrett, B., Griffiths, A., Schreiber, M., Ellison, N., Mercer, C., Bouton, J., Ong, B., Forster, J., Sawbridge, T., Spangenberg, G., Bryan, G. And Woodfield, D. (2004). A microsatellite map of white clover. Theoretical and Applied Genetics 109: 596-608. ",
author = "N. Casey and D. Milbourne and M. Febrer and M. Abberton and G. Jenkins and S. Barth and D. Thorogood",
note = "http://www.eucarpia.org/01sections/foddercrops/section_meetings2/Bookofabstracts2009.pdf; 18th Meeting of the Eucarpia Fodder Crops and Amenity Grasses Section ; Conference date: 10-05-2009 Through 14-05-2009",
year = "2009",
language = "English",
pages = "118--118",

}

RIS (suitable for import to EndNote) - Download

TY - CONF

T1 - Mapping the self-incompatibility (S) locus in white clover (Trifolium repens L.)

AU - Casey, N.

AU - Milbourne, D.

AU - Febrer, M.

AU - Abberton, M.

AU - Jenkins, G.

AU - Barth, S.

AU - Thorogood, D.

N1 - http://www.eucarpia.org/01sections/foddercrops/section_meetings2/Bookofabstracts2009.pdf

PY - 2009

Y1 - 2009

N2 - White clover (Trifolium repens L.) is a member of the Leguminosae flowering plant family and is used as a high quality forage crop capable of fixing atmospheric nitrogen. It is an amphidiploid (2n = 4x = 32) species constructed of two ancestral genomes, T. palescens and T. occidentale. T. repens possesses a self-incompatibility mechanism which prevents self-seed setting. The self-incompatibility reaction is contolled by a single poly-allelic locus expressed gametophytically in the male, where common allele specificity in pollen and stylar expressed genes results in late-acting, pre-fertilisation arrest of pollentube growth (Atwood, 1940). Based on the assumption that both parents were heterozygous for unique S-alleles, ninety four individuals of a bi-parental mapping family were classified into four intra-incompatible groupings from partial diallel crosses and pod-filling assessments six to nine days post-pollination of emasculated florets. Initial analysis of the two parental maps based on AFLP and SSR markers has identified marker associations with the S-locus attributing it to linkage group E2 as classified by Barrett et al (2004). To date, 12, 8, 6 and 16 plants have been assigned the S genotypes S13, S14, S23 and S24 respectively. Further work will place more markers on this linkage group, and polymorphisms of candidate genes/proteins will be compared to the S-locus genotypes of the characterised plants. ReferencesAtwood, S.S. (1940). Genetics of cross-incompatibility among self-incompatible plants of Trifolium repens. Journal of American Society of Agronomy 32: 955-968. Barrett, B., Griffiths, A., Schreiber, M., Ellison, N., Mercer, C., Bouton, J., Ong, B., Forster, J., Sawbridge, T., Spangenberg, G., Bryan, G. And Woodfield, D. (2004). A microsatellite map of white clover. Theoretical and Applied Genetics 109: 596-608.

AB - White clover (Trifolium repens L.) is a member of the Leguminosae flowering plant family and is used as a high quality forage crop capable of fixing atmospheric nitrogen. It is an amphidiploid (2n = 4x = 32) species constructed of two ancestral genomes, T. palescens and T. occidentale. T. repens possesses a self-incompatibility mechanism which prevents self-seed setting. The self-incompatibility reaction is contolled by a single poly-allelic locus expressed gametophytically in the male, where common allele specificity in pollen and stylar expressed genes results in late-acting, pre-fertilisation arrest of pollentube growth (Atwood, 1940). Based on the assumption that both parents were heterozygous for unique S-alleles, ninety four individuals of a bi-parental mapping family were classified into four intra-incompatible groupings from partial diallel crosses and pod-filling assessments six to nine days post-pollination of emasculated florets. Initial analysis of the two parental maps based on AFLP and SSR markers has identified marker associations with the S-locus attributing it to linkage group E2 as classified by Barrett et al (2004). To date, 12, 8, 6 and 16 plants have been assigned the S genotypes S13, S14, S23 and S24 respectively. Further work will place more markers on this linkage group, and polymorphisms of candidate genes/proteins will be compared to the S-locus genotypes of the characterised plants. ReferencesAtwood, S.S. (1940). Genetics of cross-incompatibility among self-incompatible plants of Trifolium repens. Journal of American Society of Agronomy 32: 955-968. Barrett, B., Griffiths, A., Schreiber, M., Ellison, N., Mercer, C., Bouton, J., Ong, B., Forster, J., Sawbridge, T., Spangenberg, G., Bryan, G. And Woodfield, D. (2004). A microsatellite map of white clover. Theoretical and Applied Genetics 109: 596-608.

UR - http://hdl.handle.net/2160/11133

M3 - Poster

SP - 118

EP - 118

T2 - 18th Meeting of the Eucarpia Fodder Crops and Amenity Grasses Section

Y2 - 10 May 2009 through 14 May 2009

ER -

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