Mapping grain iron and zinc content QTLs in an Iniadi-derived immortal population of pearl millet

Authors Organisations
  • Sushil Kumar(Author)
    International Crops Research Institute for the Semi-Arid Tropics
  • Charles Tom Hash(Author)
    International Crops Research Institute for the Semi-Arid Tropics
  • Thirunavukkarasu Nepolean(Author)
    International Crops Research Institute for the Semi-Arid Tropics
    Indian Agricultural Research Institute (IARI), New Delhi, India
  • Mahesh D. Mahendarkar(Author)
    International Crops Research Institute for the Semi-Arid Tropics
  • Chellapilla Tara Satyavathi(Author)
    Indian Agricultural Research Institute (IARI), New Delhi, India
  • Govind Singh(Author)
  • Abhishek Rathore(Author)
    International Crops Research Institute for the Semi-Arid Tropics
  • Rattan Yadav(Author)
  • Rajeev Gupta(Author)
    International Crops Research Institute for the Semi-Arid Tropics
  • Rakesh K. Srivastava(Author)
    International Crops Research Institute for the Semi-Arid Tropics
Type Article
Original languageEnglish
Article number9050248
Pages (from-to)1-17
Number of pages17
JournalGenes
Volume9
Issue number248
DOI
Publication statusPublished - 11 May 2018
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Abstract

Pearl millet is a climate-resilient nutritious crop requiring low inputs, and is capable of giving economic returns in marginal agro-ecologies. In this study, we report large effect iron (Fe) and zinc (Zn) content QTLs using DArT arrays and SSRs to generate a genetic linkage map using 317 RIL population derived from (ICMS 8511-S1-17-2-1-1-B-P03 × AIMP 92901-S1-183-2-2-B-08) cross. The
base map (7 LGs) of 196 loci was 964.2 cM (Haldane). AIMP 92901-S1-183-2-2-B-08 is a high grain Fe and Zn line, an Iniadi parent tracing its origin to the Togolese Republic, West Africa. QTL analysis revealed a large number of QTLs for grain iron (Fe) and zinc (Zn) content. The concentration of grain Fe in the RIL population ranged between 20 and 131 ppm, and Zn from 18-110 ppm. A total
of 19 QTLs for Fe and Zn were detected, of which 11 were for Fe and 8 were for Zn. The portion of observed phenotypic variance explained by different QTLs for grain Fe and Zn concentrations varied between 9.0-31.9% (cumulative 74%) and 9.4-30.4% (cumulative 65%), respectively. Three large effect QTLs for both minerals were co-mapped in this population - one on Linkage group (LG) 1 and the remaining two on LG7. The favourable alleles for QTLs of both the mineral micronutrients were contributed by the male parent (AIMP 92901-deriv-08). Three putative epistasis interactions were observed for Fe while single digenic
interaction was for Zn. The reported QTLs may be useful in marker-assisted selection programs for seed and restorer parent breeding and population improvement programs in pearl millet.

Keywords

  • Pearl millet , QTL mapping , Iron and Zinc content , DArT , SSR , RILs , QTL