Manipulating the phenolic acid content and digestibility of Italian ryegrass (Lolium multiflorum) by vacuolar targeted expression of a fungal ferulic acid esterase

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Manipulating the phenolic acid content and digestibility of Italian ryegrass (Lolium multiflorum) by vacuolar targeted expression of a fungal ferulic acid esterase. / Buanafina, Marcia M. de O; Langdon, Timothy; Hauck, Barbara; Dalton, Susan; Morris, Phillip.

In: Applied Biochemistry and Biotechnology, Vol. 130, No. 1-3, 2006, p. 416-426.

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Buanafina, Marcia M. de O ; Langdon, Timothy ; Hauck, Barbara ; Dalton, Susan ; Morris, Phillip. / Manipulating the phenolic acid content and digestibility of Italian ryegrass (Lolium multiflorum) by vacuolar targeted expression of a fungal ferulic acid esterase. In: Applied Biochemistry and Biotechnology. 2006 ; Vol. 130, No. 1-3. pp. 416-426.

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@article{c65ee1cfec9b4c8b9b0e65a630fa6fa6,
title = "Manipulating the phenolic acid content and digestibility of Italian ryegrass (Lolium multiflorum) by vacuolar targeted expression of a fungal ferulic acid esterase",
abstract = "In grass cell walls, ferulic acid esters linked to arabinosyl residues in arabinoxylans play a key role in crosslinking hemicellulose. Although such crosslinks have a number of important roles in the cell wall, they also hinder the rate and extent of cell wall degradation by ruminant microbes and by fungal glycohydrolyase enzymes. Ferulic acid esterase (FAE) can release both monomeric and dimeric ferulic acids from arabinoxylans making the cell wall more susceptible to further enzymatic attack. Transgenic plants of Lolium multiflorum expressing a ferulic acid esterase gene from Aspergillus niger, targeted to the vacuole under a constitutive rice actin promoter, have been produced following microprojectile bombardment of embryogenic cell cultures. The level of FAE activity was found to vary with leaf age and was highest in young leaves. FAE expression resulted in the release of monomeric and dimeric ferulic acids from cell walls on cell death and this was enhanced severalfold by the addition of exogenous β-1,4-endoxylanase. We also show that a number of plants expressing FAE had reduced levels of cell wall esterified monomeric and dimeric ferulates and increased in vitro dry-matter digestibility compared with nontransformed plants.",
keywords = "constitutive vacuolar-targeted expression, digestibility, ferulic acid esterase, L. multiflorum, transgenic grasses",
author = "Buanafina, {Marcia M. de O} and Timothy Langdon and Barbara Hauck and Susan Dalton and Phillip Morris",
year = "2006",
doi = "10.1385/ABAB:130:1:416",
language = "English",
volume = "130",
pages = "416--426",
journal = "Applied Biochemistry and Biotechnology",
issn = "0273-2289",
publisher = "Springer Nature",
number = "1-3",

}

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TY - JOUR

T1 - Manipulating the phenolic acid content and digestibility of Italian ryegrass (Lolium multiflorum) by vacuolar targeted expression of a fungal ferulic acid esterase

AU - Buanafina, Marcia M. de O

AU - Langdon, Timothy

AU - Hauck, Barbara

AU - Dalton, Susan

AU - Morris, Phillip

PY - 2006

Y1 - 2006

N2 - In grass cell walls, ferulic acid esters linked to arabinosyl residues in arabinoxylans play a key role in crosslinking hemicellulose. Although such crosslinks have a number of important roles in the cell wall, they also hinder the rate and extent of cell wall degradation by ruminant microbes and by fungal glycohydrolyase enzymes. Ferulic acid esterase (FAE) can release both monomeric and dimeric ferulic acids from arabinoxylans making the cell wall more susceptible to further enzymatic attack. Transgenic plants of Lolium multiflorum expressing a ferulic acid esterase gene from Aspergillus niger, targeted to the vacuole under a constitutive rice actin promoter, have been produced following microprojectile bombardment of embryogenic cell cultures. The level of FAE activity was found to vary with leaf age and was highest in young leaves. FAE expression resulted in the release of monomeric and dimeric ferulic acids from cell walls on cell death and this was enhanced severalfold by the addition of exogenous β-1,4-endoxylanase. We also show that a number of plants expressing FAE had reduced levels of cell wall esterified monomeric and dimeric ferulates and increased in vitro dry-matter digestibility compared with nontransformed plants.

AB - In grass cell walls, ferulic acid esters linked to arabinosyl residues in arabinoxylans play a key role in crosslinking hemicellulose. Although such crosslinks have a number of important roles in the cell wall, they also hinder the rate and extent of cell wall degradation by ruminant microbes and by fungal glycohydrolyase enzymes. Ferulic acid esterase (FAE) can release both monomeric and dimeric ferulic acids from arabinoxylans making the cell wall more susceptible to further enzymatic attack. Transgenic plants of Lolium multiflorum expressing a ferulic acid esterase gene from Aspergillus niger, targeted to the vacuole under a constitutive rice actin promoter, have been produced following microprojectile bombardment of embryogenic cell cultures. The level of FAE activity was found to vary with leaf age and was highest in young leaves. FAE expression resulted in the release of monomeric and dimeric ferulic acids from cell walls on cell death and this was enhanced severalfold by the addition of exogenous β-1,4-endoxylanase. We also show that a number of plants expressing FAE had reduced levels of cell wall esterified monomeric and dimeric ferulates and increased in vitro dry-matter digestibility compared with nontransformed plants.

KW - constitutive vacuolar-targeted expression

KW - digestibility

KW - ferulic acid esterase

KW - L. multiflorum

KW - transgenic grasses

UR - http://hdl.handle.net/2160/36519

U2 - 10.1385/ABAB:130:1:416

DO - 10.1385/ABAB:130:1:416

M3 - Article

VL - 130

SP - 416

EP - 426

JO - Applied Biochemistry and Biotechnology

JF - Applied Biochemistry and Biotechnology

SN - 0273-2289

IS - 1-3

ER -

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