Leaf senescence is delayed in maize expressing the Agrobacterium IPT gene under the control of a novel maize senescence-enhanced promoter

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Leaf senescence is delayed in maize expressing the Agrobacterium IPT gene under the control of a novel maize senescence-enhanced promoter. / Robson, Paul R. H.; Donnison, Iain S.; Wang, Kan; Frame, Bronwyn; Pegg, Sue Ellen; Thomas, Ann M.; Thomas, Howard.

In: Plant Biotechnology Journal, Vol. 2, No. 2, 03.2004, p. 101-112.

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@article{289c4db5e15745b2890848425b100f91,
title = "Leaf senescence is delayed in maize expressing the Agrobacterium IPT gene under the control of a novel maize senescence-enhanced promoter",
abstract = "We have genetically modified maize plants to delay leaf senescence. A senescence-enhanced promoter from maize (PSEE1) was used to drive expression of the Agrobacterium cytokinin biosynthesis gene IPT in senescing leaf tissue. Three maize lines expressing IPT from PSEE1, Sg1, Sg2 and Sg3, were analysed in detail, representing mild, intermediate and extreme expression, respectively, of the delayed-senescence phenotype. Backcross populations segregating for the presence or absence of the PSEE1XbaIPTNOS transgene also simultaneously segregated for the senescence phenotype. At the time of ear leaf emergence, individuals of lines Sg1 and Sg2 segregating for the presence of the transgene carried about three fewer senescing leaves than control (transgene-minus) segregants, and IPT transcript levels were higher in leaves at incipient senescence than in young leaves. Leaves of transgenic Sg3 plants were significantly greener than controls and progressed directly from fully green to bleached and dead without an intervening yellowing phase. IPT transcript abundance in this line was not related to the initiation of senescence. Extended greenness was accompanied by a delay in the loss of photosynthetic capacity with leaf age. The delayed-senescence trait was associated with relatively minor changes in morphology and development. The phenotype was particularly emphasized in plants grown in low soil nitrogen. The reduced ability of the extreme transgenic line Sg3 to recycle internal nitrogen from senescing lower leaves accounted for significant chlorosis in emerging younger leaves when plants were grown in low nutrient conditions. This study demonstrates that the agronomically important delayed-senescence ('stay-green') trait can be engineered into a monocot crop, and is the first example outside Arabidopsis of senescence modification using a homologous senescence-enhanced promoter.",
keywords = "IPT, promoter, SEE1, senescence, stay-green, Zea mays",
author = "Robson, {Paul R. H.} and Donnison, {Iain S.} and Kan Wang and Bronwyn Frame and Pegg, {Sue Ellen} and Thomas, {Ann M.} and Howard Thomas",
note = "Robson, P. R. H., Donnison, I. S., Wang, K., Frame, B., Pegg, S. E., Thomas, A., Thomas, Howard (2004). Leaf senescence is delayed in maize expressing the Agrobacterium IPT gene under the control of a novel maize senescence-enhanced promoter. Plant Biotechnology Journal, 2 (2), 101-112. Sponsorship: This research was funded by BBSRC (Biotechnology and Biological Sciences Research Council) under the Resource Allocation and Stress in Plants initiative (RASP project RSP07828) and by Advanta Seeds.",
year = "2004",
month = mar,
doi = "10.1046/j.1467-7652.2004.00054.x",
language = "English",
volume = "2",
pages = "101--112",
journal = "Plant Biotechnology Journal",
issn = "1467-7644",
publisher = "Wiley",
number = "2",

}

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TY - JOUR

T1 - Leaf senescence is delayed in maize expressing the Agrobacterium IPT gene under the control of a novel maize senescence-enhanced promoter

AU - Robson, Paul R. H.

AU - Donnison, Iain S.

AU - Wang, Kan

AU - Frame, Bronwyn

AU - Pegg, Sue Ellen

AU - Thomas, Ann M.

AU - Thomas, Howard

N1 - Robson, P. R. H., Donnison, I. S., Wang, K., Frame, B., Pegg, S. E., Thomas, A., Thomas, Howard (2004). Leaf senescence is delayed in maize expressing the Agrobacterium IPT gene under the control of a novel maize senescence-enhanced promoter. Plant Biotechnology Journal, 2 (2), 101-112. Sponsorship: This research was funded by BBSRC (Biotechnology and Biological Sciences Research Council) under the Resource Allocation and Stress in Plants initiative (RASP project RSP07828) and by Advanta Seeds.

PY - 2004/3

Y1 - 2004/3

N2 - We have genetically modified maize plants to delay leaf senescence. A senescence-enhanced promoter from maize (PSEE1) was used to drive expression of the Agrobacterium cytokinin biosynthesis gene IPT in senescing leaf tissue. Three maize lines expressing IPT from PSEE1, Sg1, Sg2 and Sg3, were analysed in detail, representing mild, intermediate and extreme expression, respectively, of the delayed-senescence phenotype. Backcross populations segregating for the presence or absence of the PSEE1XbaIPTNOS transgene also simultaneously segregated for the senescence phenotype. At the time of ear leaf emergence, individuals of lines Sg1 and Sg2 segregating for the presence of the transgene carried about three fewer senescing leaves than control (transgene-minus) segregants, and IPT transcript levels were higher in leaves at incipient senescence than in young leaves. Leaves of transgenic Sg3 plants were significantly greener than controls and progressed directly from fully green to bleached and dead without an intervening yellowing phase. IPT transcript abundance in this line was not related to the initiation of senescence. Extended greenness was accompanied by a delay in the loss of photosynthetic capacity with leaf age. The delayed-senescence trait was associated with relatively minor changes in morphology and development. The phenotype was particularly emphasized in plants grown in low soil nitrogen. The reduced ability of the extreme transgenic line Sg3 to recycle internal nitrogen from senescing lower leaves accounted for significant chlorosis in emerging younger leaves when plants were grown in low nutrient conditions. This study demonstrates that the agronomically important delayed-senescence ('stay-green') trait can be engineered into a monocot crop, and is the first example outside Arabidopsis of senescence modification using a homologous senescence-enhanced promoter.

AB - We have genetically modified maize plants to delay leaf senescence. A senescence-enhanced promoter from maize (PSEE1) was used to drive expression of the Agrobacterium cytokinin biosynthesis gene IPT in senescing leaf tissue. Three maize lines expressing IPT from PSEE1, Sg1, Sg2 and Sg3, were analysed in detail, representing mild, intermediate and extreme expression, respectively, of the delayed-senescence phenotype. Backcross populations segregating for the presence or absence of the PSEE1XbaIPTNOS transgene also simultaneously segregated for the senescence phenotype. At the time of ear leaf emergence, individuals of lines Sg1 and Sg2 segregating for the presence of the transgene carried about three fewer senescing leaves than control (transgene-minus) segregants, and IPT transcript levels were higher in leaves at incipient senescence than in young leaves. Leaves of transgenic Sg3 plants were significantly greener than controls and progressed directly from fully green to bleached and dead without an intervening yellowing phase. IPT transcript abundance in this line was not related to the initiation of senescence. Extended greenness was accompanied by a delay in the loss of photosynthetic capacity with leaf age. The delayed-senescence trait was associated with relatively minor changes in morphology and development. The phenotype was particularly emphasized in plants grown in low soil nitrogen. The reduced ability of the extreme transgenic line Sg3 to recycle internal nitrogen from senescing lower leaves accounted for significant chlorosis in emerging younger leaves when plants were grown in low nutrient conditions. This study demonstrates that the agronomically important delayed-senescence ('stay-green') trait can be engineered into a monocot crop, and is the first example outside Arabidopsis of senescence modification using a homologous senescence-enhanced promoter.

KW - IPT

KW - promoter

KW - SEE1

KW - senescence

KW - stay-green

KW - Zea mays

U2 - 10.1046/j.1467-7652.2004.00054.x

DO - 10.1046/j.1467-7652.2004.00054.x

M3 - Article

C2 - 17147603

VL - 2

SP - 101

EP - 112

JO - Plant Biotechnology Journal

JF - Plant Biotechnology Journal

SN - 1467-7644

IS - 2

ER -

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