Isolation and characterization of a genomic region upstream from the ovine Na+/D-glucose cotransporter (SGLT1) cDNA

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Isolation and characterization of a genomic region upstream from the ovine Na+/D-glucose cotransporter (SGLT1) cDNA. / Wood, I. Stuart; Allison, Gordon; Shirazi-Beechey, Soraya P.

In: Biochemical and Biophysical Research Communications, Vol. 257, No. 2, 13.04.1999, p. 533-537.

Research output: Contribution to journalArticle

Harvard

Wood, IS, Allison, G & Shirazi-Beechey, SP 1999, 'Isolation and characterization of a genomic region upstream from the ovine Na+/D-glucose cotransporter (SGLT1) cDNA', Biochemical and Biophysical Research Communications, vol. 257, no. 2, pp. 533-537. https://doi.org/10.1006/bbrc.1999.0526

APA

Wood, I. S., Allison, G., & Shirazi-Beechey, S. P. (1999). Isolation and characterization of a genomic region upstream from the ovine Na+/D-glucose cotransporter (SGLT1) cDNA. Biochemical and Biophysical Research Communications, 257(2), 533-537. https://doi.org/10.1006/bbrc.1999.0526

Vancouver

Wood IS, Allison G, Shirazi-Beechey SP. Isolation and characterization of a genomic region upstream from the ovine Na+/D-glucose cotransporter (SGLT1) cDNA. Biochemical and Biophysical Research Communications. 1999 Apr 13;257(2):533-537. https://doi.org/10.1006/bbrc.1999.0526

Author

Wood, I. Stuart ; Allison, Gordon ; Shirazi-Beechey, Soraya P. / Isolation and characterization of a genomic region upstream from the ovine Na+/D-glucose cotransporter (SGLT1) cDNA. In: Biochemical and Biophysical Research Communications. 1999 ; Vol. 257, No. 2. pp. 533-537.

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@article{113028f133c948f5ab54201f98e4d214,
title = "Isolation and characterization of a genomic region upstream from the ovine Na+/D-glucose cotransporter (SGLT1) cDNA",
abstract = "d-Glucose and non-metabolisable analogues of D-glucose regulate the expression of intestinal SGLT1 at both transcriptional and post-transcriptional levels. In order to investigate the molecular mechanisms involved in the transcriptional regulation of the ovine intestinal SGLT1 gene, we have isolated an upstream element of about 1 kb in size. This DNA fragment contains a TATA box motif, 48 bp upstream of the transcriptional start site and includes transcription factor binding sites for HNF-1 and AP-2. We have shown that the ovine SGLT1 promoter fragment can drive the transcription of a reporter gene when transfected into the epithelial cell lines STC-1 and LLC-PK1, which endogenously express SGLT1. Deletion analyses of the promoter indicate that −66/+21 bp proximal sequence directs the highest level of reporter gene activity. There are one and possibly two sites of transcriptional suppression.",
keywords = "SGLT1, promoter, transcription, ovine intestine",
author = "Wood, {I. Stuart} and Gordon Allison and Shirazi-Beechey, {Soraya P.}",
year = "1999",
month = apr,
day = "13",
doi = "10.1006/bbrc.1999.0526",
language = "English",
volume = "257",
pages = "533--537",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier",
number = "2",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Isolation and characterization of a genomic region upstream from the ovine Na+/D-glucose cotransporter (SGLT1) cDNA

AU - Wood, I. Stuart

AU - Allison, Gordon

AU - Shirazi-Beechey, Soraya P.

PY - 1999/4/13

Y1 - 1999/4/13

N2 - d-Glucose and non-metabolisable analogues of D-glucose regulate the expression of intestinal SGLT1 at both transcriptional and post-transcriptional levels. In order to investigate the molecular mechanisms involved in the transcriptional regulation of the ovine intestinal SGLT1 gene, we have isolated an upstream element of about 1 kb in size. This DNA fragment contains a TATA box motif, 48 bp upstream of the transcriptional start site and includes transcription factor binding sites for HNF-1 and AP-2. We have shown that the ovine SGLT1 promoter fragment can drive the transcription of a reporter gene when transfected into the epithelial cell lines STC-1 and LLC-PK1, which endogenously express SGLT1. Deletion analyses of the promoter indicate that −66/+21 bp proximal sequence directs the highest level of reporter gene activity. There are one and possibly two sites of transcriptional suppression.

AB - d-Glucose and non-metabolisable analogues of D-glucose regulate the expression of intestinal SGLT1 at both transcriptional and post-transcriptional levels. In order to investigate the molecular mechanisms involved in the transcriptional regulation of the ovine intestinal SGLT1 gene, we have isolated an upstream element of about 1 kb in size. This DNA fragment contains a TATA box motif, 48 bp upstream of the transcriptional start site and includes transcription factor binding sites for HNF-1 and AP-2. We have shown that the ovine SGLT1 promoter fragment can drive the transcription of a reporter gene when transfected into the epithelial cell lines STC-1 and LLC-PK1, which endogenously express SGLT1. Deletion analyses of the promoter indicate that −66/+21 bp proximal sequence directs the highest level of reporter gene activity. There are one and possibly two sites of transcriptional suppression.

KW - SGLT1

KW - promoter

KW - transcription

KW - ovine intestine

UR - http://hdl.handle.net/2160/43185

U2 - 10.1006/bbrc.1999.0526

DO - 10.1006/bbrc.1999.0526

M3 - Article

VL - 257

SP - 533

EP - 537

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -

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