Isolation and characterisation of polymorphic microsatellite loci for studies of the big blue octopus, Octopus cyanea

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Isolation and characterisation of polymorphic microsatellite loci for studies of the big blue octopus, Octopus cyanea. / McKeown, Niall; Taylor, Amy L.; Shaw, Paul.

In: Marine Biodiversity, Vol. 48, No. 4, 29.04.2017, p. 2233-2235.

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@article{d4bcc5dc9b19445d92f0a3af8481b769,
title = "Isolation and characterisation of polymorphic microsatellite loci for studies of the big blue octopus, Octopus cyanea",
abstract = "The big blue octopus, Octopus cyanea, occurs on coral reefs throughout the Indo-Pacific region from East Africa to the Hawaiian Islands, wherein it is of great ecological and socio-economic importance. However, many components of its intraspecific biodiversity, such as population structure, are unresolved due to a lack of informative genetic markers. To address this issue, which may compromise conservation and sustainability efforts, the development and characterisation of the first species-specific microsatellite loci for O. cyanea are described here. The eight loci were characterised by the genotyping of 40 adults from Madagascar, which revealed an average of 13.5 alleles per locus (range 9–18). The observed and expected heterozygosity per locus ranged from 0.432 to 0.949 and from 0.481 to 0.989, respectively. No evidence of linkage disequilibrium was detected between pairs of loci. Genotype proportions at six loci conformed to Hardy–Weinberg equilibrium expectations, with two loci exhibiting significant heterozygote deficits. These loci are applicable to multiple areas of eco-evolutionary research and, thus, represent a valuable resource for future studies of O. cyanea",
keywords = "marine invertebrate, genetic, biodiversity, population genetics, conservation, management",
author = "Niall McKeown and Taylor, {Amy L.} and Paul Shaw",
year = "2017",
month = apr,
day = "29",
doi = "10.1007/s12526-017-0717-0",
language = "English",
volume = "48",
pages = "2233--2235",
journal = "Marine Biodiversity",
issn = "1867-1616",
publisher = "Springer Nature",
number = "4",

}

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TY - JOUR

T1 - Isolation and characterisation of polymorphic microsatellite loci for studies of the big blue octopus, Octopus cyanea

AU - McKeown, Niall

AU - Taylor, Amy L.

AU - Shaw, Paul

PY - 2017/4/29

Y1 - 2017/4/29

N2 - The big blue octopus, Octopus cyanea, occurs on coral reefs throughout the Indo-Pacific region from East Africa to the Hawaiian Islands, wherein it is of great ecological and socio-economic importance. However, many components of its intraspecific biodiversity, such as population structure, are unresolved due to a lack of informative genetic markers. To address this issue, which may compromise conservation and sustainability efforts, the development and characterisation of the first species-specific microsatellite loci for O. cyanea are described here. The eight loci were characterised by the genotyping of 40 adults from Madagascar, which revealed an average of 13.5 alleles per locus (range 9–18). The observed and expected heterozygosity per locus ranged from 0.432 to 0.949 and from 0.481 to 0.989, respectively. No evidence of linkage disequilibrium was detected between pairs of loci. Genotype proportions at six loci conformed to Hardy–Weinberg equilibrium expectations, with two loci exhibiting significant heterozygote deficits. These loci are applicable to multiple areas of eco-evolutionary research and, thus, represent a valuable resource for future studies of O. cyanea

AB - The big blue octopus, Octopus cyanea, occurs on coral reefs throughout the Indo-Pacific region from East Africa to the Hawaiian Islands, wherein it is of great ecological and socio-economic importance. However, many components of its intraspecific biodiversity, such as population structure, are unresolved due to a lack of informative genetic markers. To address this issue, which may compromise conservation and sustainability efforts, the development and characterisation of the first species-specific microsatellite loci for O. cyanea are described here. The eight loci were characterised by the genotyping of 40 adults from Madagascar, which revealed an average of 13.5 alleles per locus (range 9–18). The observed and expected heterozygosity per locus ranged from 0.432 to 0.949 and from 0.481 to 0.989, respectively. No evidence of linkage disequilibrium was detected between pairs of loci. Genotype proportions at six loci conformed to Hardy–Weinberg equilibrium expectations, with two loci exhibiting significant heterozygote deficits. These loci are applicable to multiple areas of eco-evolutionary research and, thus, represent a valuable resource for future studies of O. cyanea

KW - marine invertebrate

KW - genetic

KW - biodiversity

KW - population genetics

KW - conservation

KW - management

UR - http://hdl.handle.net/2160/45095

U2 - 10.1007/s12526-017-0717-0

DO - 10.1007/s12526-017-0717-0

M3 - Article

VL - 48

SP - 2233

EP - 2235

JO - Marine Biodiversity

JF - Marine Biodiversity

SN - 1867-1616

IS - 4

ER -

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