Introgression of Aegilops speltoides segments in Triticum aestivum and the effect of the gametocidal genes

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Introgression of Aegilops speltoides segments in Triticum aestivum and the effect of the gametocidal genes. / King, Julie; Grewal, Surbhi ; Yang, Cai-yun; Hubbart Edwards, Stella ; Scholefield, Duncan; Ashling, Stephen; Harper, John; Allen, Alexandra; Edwards, Keith J.; Burridge, Amanda; King, Ian Philip.

In: Annals of Botany, Vol. 121, No. 2, 23.01.2018, p. 229-240.

Research output: Contribution to journalArticle

Harvard

King, J, Grewal, S, Yang, C, Hubbart Edwards, S, Scholefield, D, Ashling, S, Harper, J, Allen, A, Edwards, KJ, Burridge, A & King, IP 2018, 'Introgression of Aegilops speltoides segments in Triticum aestivum and the effect of the gametocidal genes' Annals of Botany, vol. 121, no. 2, pp. 229-240. https://doi.org/10.1093/aob/mcx149

APA

King, J., Grewal, S., Yang, C., Hubbart Edwards, S., Scholefield, D., Ashling, S., ... King, I. P. (2018). Introgression of Aegilops speltoides segments in Triticum aestivum and the effect of the gametocidal genes. Annals of Botany, 121(2), 229-240. https://doi.org/10.1093/aob/mcx149

Vancouver

King J, Grewal S, Yang C, Hubbart Edwards S, Scholefield D, Ashling S et al. Introgression of Aegilops speltoides segments in Triticum aestivum and the effect of the gametocidal genes. Annals of Botany. 2018 Jan 23;121(2):229-240. https://doi.org/10.1093/aob/mcx149

Author

King, Julie ; Grewal, Surbhi ; Yang, Cai-yun ; Hubbart Edwards, Stella ; Scholefield, Duncan ; Ashling, Stephen ; Harper, John ; Allen, Alexandra ; Edwards, Keith J. ; Burridge, Amanda ; King, Ian Philip. / Introgression of Aegilops speltoides segments in Triticum aestivum and the effect of the gametocidal genes. In: Annals of Botany. 2018 ; Vol. 121, No. 2. pp. 229-240.

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@article{31ce7ed132964612a737eaf49bb54b00,
title = "Introgression of Aegilops speltoides segments in Triticum aestivum and the effect of the gametocidal genes",
abstract = "Background and AimsBread wheat (Triticum aestivum) has been through a severe genetic bottleneck as a result of its evolution and domestication. It is therefore essential that new sources of genetic variation are generated and utilized. This study aimed to generate genome-wide introgressed segments from Aegilops speltoides. Introgressions generated from this research will be made available for phenotypic analysis. MethodsAegilops speltoides was crossed as the male parent to T. aestivum ‘Paragon’. The interspecific hybrids were then backcrossed to Paragon. Introgressions were detected and characterized using the Affymetrix Axiom Array and genomic in situ hybridization (GISH).Key ResultsRecombination in the gametes of the F1 hybrids was at a level where it was possible to generate a genetic linkage map of Ae. speltoides. This was used to identify 294 wheat/Ae. speltoides introgressions. Introgressions from all seven linkage groups of Ae. speltoides were found, including both large and small segments. Comparative analysis showed that overall macro-synteny is conserved between Ae. speltoides and T. aestivum, but that Ae. speltoides does not contain the 4A/5A/7B translocations present in wheat. Aegilops speltoides has been reported to carry gametocidal genes, i.e. genes that ensure their transmission through the gametes to the next generation. Transmission rates of the seven Ae. speltoides linkage groups introgressed into wheat varied. A 100 {\%} transmission rate of linkage group 2 demonstrates the presence of the gametocidal genes on this chromosome.ConclusionsA high level of recombination occurs between the chromosomes of wheat and Ae. speltoides, leading to the generation of large numbers of introgressions with the potential for exploitation in breeding programmes. Due to the gametocidal genes, all germplasm developed will always contain a segment from Ae. speltoides linkage group 2S, in addition to an introgression from any other linkage group",
keywords = "Aegilops speltoides, Triticum aestivum, introgression, recombination, comparative synteny, gametocidal, genetic linkage mapping, GISH, chromosome transmission",
author = "Julie King and Surbhi Grewal and Cai-yun Yang and {Hubbart Edwards}, Stella and Duncan Scholefield and Stephen Ashling and John Harper and Alexandra Allen and Edwards, {Keith J.} and Amanda Burridge and King, {Ian Philip}",
year = "2018",
month = "1",
day = "23",
doi = "10.1093/aob/mcx149",
language = "English",
volume = "121",
pages = "229--240",
journal = "Annals of Botany",
issn = "0305-7364",
publisher = "Oxford University Press",
number = "2",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Introgression of Aegilops speltoides segments in Triticum aestivum and the effect of the gametocidal genes

AU - King, Julie

AU - Grewal, Surbhi

AU - Yang, Cai-yun

AU - Hubbart Edwards, Stella

AU - Scholefield, Duncan

AU - Ashling, Stephen

AU - Harper, John

AU - Allen, Alexandra

AU - Edwards, Keith J.

AU - Burridge, Amanda

AU - King, Ian Philip

PY - 2018/1/23

Y1 - 2018/1/23

N2 - Background and AimsBread wheat (Triticum aestivum) has been through a severe genetic bottleneck as a result of its evolution and domestication. It is therefore essential that new sources of genetic variation are generated and utilized. This study aimed to generate genome-wide introgressed segments from Aegilops speltoides. Introgressions generated from this research will be made available for phenotypic analysis. MethodsAegilops speltoides was crossed as the male parent to T. aestivum ‘Paragon’. The interspecific hybrids were then backcrossed to Paragon. Introgressions were detected and characterized using the Affymetrix Axiom Array and genomic in situ hybridization (GISH).Key ResultsRecombination in the gametes of the F1 hybrids was at a level where it was possible to generate a genetic linkage map of Ae. speltoides. This was used to identify 294 wheat/Ae. speltoides introgressions. Introgressions from all seven linkage groups of Ae. speltoides were found, including both large and small segments. Comparative analysis showed that overall macro-synteny is conserved between Ae. speltoides and T. aestivum, but that Ae. speltoides does not contain the 4A/5A/7B translocations present in wheat. Aegilops speltoides has been reported to carry gametocidal genes, i.e. genes that ensure their transmission through the gametes to the next generation. Transmission rates of the seven Ae. speltoides linkage groups introgressed into wheat varied. A 100 % transmission rate of linkage group 2 demonstrates the presence of the gametocidal genes on this chromosome.ConclusionsA high level of recombination occurs between the chromosomes of wheat and Ae. speltoides, leading to the generation of large numbers of introgressions with the potential for exploitation in breeding programmes. Due to the gametocidal genes, all germplasm developed will always contain a segment from Ae. speltoides linkage group 2S, in addition to an introgression from any other linkage group

AB - Background and AimsBread wheat (Triticum aestivum) has been through a severe genetic bottleneck as a result of its evolution and domestication. It is therefore essential that new sources of genetic variation are generated and utilized. This study aimed to generate genome-wide introgressed segments from Aegilops speltoides. Introgressions generated from this research will be made available for phenotypic analysis. MethodsAegilops speltoides was crossed as the male parent to T. aestivum ‘Paragon’. The interspecific hybrids were then backcrossed to Paragon. Introgressions were detected and characterized using the Affymetrix Axiom Array and genomic in situ hybridization (GISH).Key ResultsRecombination in the gametes of the F1 hybrids was at a level where it was possible to generate a genetic linkage map of Ae. speltoides. This was used to identify 294 wheat/Ae. speltoides introgressions. Introgressions from all seven linkage groups of Ae. speltoides were found, including both large and small segments. Comparative analysis showed that overall macro-synteny is conserved between Ae. speltoides and T. aestivum, but that Ae. speltoides does not contain the 4A/5A/7B translocations present in wheat. Aegilops speltoides has been reported to carry gametocidal genes, i.e. genes that ensure their transmission through the gametes to the next generation. Transmission rates of the seven Ae. speltoides linkage groups introgressed into wheat varied. A 100 % transmission rate of linkage group 2 demonstrates the presence of the gametocidal genes on this chromosome.ConclusionsA high level of recombination occurs between the chromosomes of wheat and Ae. speltoides, leading to the generation of large numbers of introgressions with the potential for exploitation in breeding programmes. Due to the gametocidal genes, all germplasm developed will always contain a segment from Ae. speltoides linkage group 2S, in addition to an introgression from any other linkage group

KW - Aegilops speltoides

KW - Triticum aestivum

KW - introgression

KW - recombination

KW - comparative synteny

KW - gametocidal

KW - genetic linkage mapping

KW - GISH

KW - chromosome transmission

U2 - 10.1093/aob/mcx149

DO - 10.1093/aob/mcx149

M3 - Article

VL - 121

SP - 229

EP - 240

JO - Annals of Botany

JF - Annals of Botany

SN - 0305-7364

IS - 2

ER -

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