Initial biochar effects on plant productivity derive from N fertilizations

Authors Organisations
  • Simon Jeffery(Author)
    Harper Adams University
  • Ilse Memelink(Author)
    Wageningen University and Research Centre
  • Edward Hodgson(Author)
  • Sian Jones(Author)
  • Tess F. J. van de Voorde(Author)
    Wageningen University and Research Centre
  • T. Martijn Bezemer(Author)
    Netherlands Institute of Ecology (NIOO-KNAW)
    Leiden University
  • Liesje Mommer(Author)
    Wageningen University and Research Centre
  • Jan Willem van Groenigen(Author)
    Wageningen University and Research Centre
Type Article
Original languageEnglish
Pages (from-to)435-448
Number of pages14
JournalPlant and Soil
Issue number1-2
Early online date13 Jan 2017
Publication statusPublished - 01 Jun 2017
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Background and aim
Biochar application to soil is widely claimed to increase plant productivity. However, the underlying mechanisms are still not conclusively described. Here, we aim to elucidate these mechanisms using stable isotope probing.

We conducted two experiments with uniquely double-labelled (15N and 13C) biochar and its feedstock (residue), applied separately at 15 Mg ha−1. Both experiments contained three treatments: biochar amendment (Biochar), unpyrolysed residue amendment (Residue) and a no addition control (Control). Experiment I was a 119 day pot experiment seeded with Lolium perenne. Experiment II was a 71 day incubation experiment without plants in which CO2 and N2O fluxes were measured.

Both Biochar and Residue significantly increased aboveground productivity compared to Control (140% and 160%, respectively). Initial N immobilisation was stimulated in Residue, whereas not in Biochar. 13C–CO2 analysis confirmed that biochar was significantly more recalcitrant than residue. 15N analysis showed that 2% and 0.3% of grass N was derived from the amended material in Residue and Biochar, respectively.

Our results suggest that biochar-induced yield increases derive from a combination of reduced N immobilization and a moderate N fertilization effect. Although in the short term biochar might offer benefits compared to residue incorporation, it is unlikely that biochar yield gains will be sustainable for the decades to centuries that biochar C can be expected to reside in soil.


  • pyrolysis, organic amendment, stable isotopes, c dynamics, N immobilisation, greenhouse gases