In vitro analysis of the effect of supplementation with activated charcoal on the equine hindgut

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In vitro analysis of the effect of supplementation with activated charcoal on the equine hindgut. / Edmunds, Jody Leigh; Worgan, Hilary; Dougal, Kirsty; Girdwood, Susan; Douglas, Jamie-Leigh; McEwan, Neil.

In: Journal of Equine Science, Vol. 27, No. 2, 21.06.2016, p. 49-55.

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Harvard

Edmunds, JL, Worgan, H, Dougal, K, Girdwood, S, Douglas, J-L & McEwan, N 2016, 'In vitro analysis of the effect of supplementation with activated charcoal on the equine hindgut', Journal of Equine Science, vol. 27, no. 2, pp. 49-55. https://doi.org/10.1294/jes.27.49

APA

Edmunds, J. L., Worgan, H., Dougal, K., Girdwood, S., Douglas, J-L., & McEwan, N. (2016). In vitro analysis of the effect of supplementation with activated charcoal on the equine hindgut. Journal of Equine Science, 27(2), 49-55. https://doi.org/10.1294/jes.27.49

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Author

Edmunds, Jody Leigh ; Worgan, Hilary ; Dougal, Kirsty ; Girdwood, Susan ; Douglas, Jamie-Leigh ; McEwan, Neil. / In vitro analysis of the effect of supplementation with activated charcoal on the equine hindgut. In: Journal of Equine Science. 2016 ; Vol. 27, No. 2. pp. 49-55.

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@article{5d9bc5c7e8ff4ea5a0a09d26817247b0,
title = "In vitro analysis of the effect of supplementation with activated charcoal on the equine hindgut",
abstract = "The present study uses in vitro analytical techniques to investigate the effect of activated charcoal on the microbial community of the equine hindgut and the metabolites they produce. Incubations were performed in Wheaton bottles using a 50 ml incubation of a high-energy feed or a low-energy feed, plus bottles with no added food source, together with five levels of activated charcoal (0, 10, 25, 50 or 100 mg per bottle) and fecal samples as a bacterial inoculum. Using this method the rate of gas production, volatile fatty acid and ammonia concentrations, and pH values were analyzed and found to vary depending on the addition of feed, but the activated charcoal had no effect (P>0.05) on any of these. It is already believed that the effect of activated charcoal as a control for toxic substances is at its highest in the foregut or midgut of animals, and therefore should have little impact on the hindgut. The data presented here suggest that if any of the activated charcoal does reach the hindgut, then it has no significant impact on the microbial community present, nor on the major metabolites produced, and so should not have a detrimental effect on the principal site of fermentation in the horse.",
keywords = "activated charcoal, digestive metabolites, horse, microbial profiles",
author = "Edmunds, {Jody Leigh} and Hilary Worgan and Kirsty Dougal and Susan Girdwood and Jamie-Leigh Douglas and Neil McEwan",
year = "2016",
month = jun,
day = "21",
doi = "10.1294/jes.27.49",
language = "English",
volume = "27",
pages = "49--55",
journal = "Journal of Equine Science",
issn = "1340-3516",
publisher = "Japanese Society of Equine Science",
number = "2",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - In vitro analysis of the effect of supplementation with activated charcoal on the equine hindgut

AU - Edmunds, Jody Leigh

AU - Worgan, Hilary

AU - Dougal, Kirsty

AU - Girdwood, Susan

AU - Douglas, Jamie-Leigh

AU - McEwan, Neil

PY - 2016/6/21

Y1 - 2016/6/21

N2 - The present study uses in vitro analytical techniques to investigate the effect of activated charcoal on the microbial community of the equine hindgut and the metabolites they produce. Incubations were performed in Wheaton bottles using a 50 ml incubation of a high-energy feed or a low-energy feed, plus bottles with no added food source, together with five levels of activated charcoal (0, 10, 25, 50 or 100 mg per bottle) and fecal samples as a bacterial inoculum. Using this method the rate of gas production, volatile fatty acid and ammonia concentrations, and pH values were analyzed and found to vary depending on the addition of feed, but the activated charcoal had no effect (P>0.05) on any of these. It is already believed that the effect of activated charcoal as a control for toxic substances is at its highest in the foregut or midgut of animals, and therefore should have little impact on the hindgut. The data presented here suggest that if any of the activated charcoal does reach the hindgut, then it has no significant impact on the microbial community present, nor on the major metabolites produced, and so should not have a detrimental effect on the principal site of fermentation in the horse.

AB - The present study uses in vitro analytical techniques to investigate the effect of activated charcoal on the microbial community of the equine hindgut and the metabolites they produce. Incubations were performed in Wheaton bottles using a 50 ml incubation of a high-energy feed or a low-energy feed, plus bottles with no added food source, together with five levels of activated charcoal (0, 10, 25, 50 or 100 mg per bottle) and fecal samples as a bacterial inoculum. Using this method the rate of gas production, volatile fatty acid and ammonia concentrations, and pH values were analyzed and found to vary depending on the addition of feed, but the activated charcoal had no effect (P>0.05) on any of these. It is already believed that the effect of activated charcoal as a control for toxic substances is at its highest in the foregut or midgut of animals, and therefore should have little impact on the hindgut. The data presented here suggest that if any of the activated charcoal does reach the hindgut, then it has no significant impact on the microbial community present, nor on the major metabolites produced, and so should not have a detrimental effect on the principal site of fermentation in the horse.

KW - activated charcoal

KW - digestive metabolites

KW - horse

KW - microbial profiles

UR - http://hdl.handle.net/2160/43581

U2 - 10.1294/jes.27.49

DO - 10.1294/jes.27.49

M3 - Article

C2 - 27330398

VL - 27

SP - 49

EP - 55

JO - Journal of Equine Science

JF - Journal of Equine Science

SN - 1340-3516

IS - 2

ER -

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