Impact of low lignin containing brown midrib sorghum mutants to harness biohydrogen production using mixed anaerobic consortia

Authors Organisations
  • R. S. Prakasham(Author)
    Indian Institute of Chemical Technology
  • P. Brahmaiah(Author)
    Indian Institute of Chemical Technology
  • D. Nagaiah(Author)
    Indian Institute of Chemical Technology
  • P. Srinivasa Rao(Author)
    International Crops Research Institute for the Semi-Arid Tropics
  • Belum V. S. Reddy(Author)
    International Crops Research Institute for the Semi-Arid Tropics
  • Rao Ravella(Author)
  • Phil J. Hobbs(Author)
    Rothamsted Research
Type Article
Original languageEnglish
Pages (from-to)3186-3190
Number of pages5
JournalInternational Journal of Hydrogen Energy
Volume37
Issue number4
DOI
Publication statusPublished - Feb 2012
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Abstract

Three low lignin containing bmr 3 derivatives, namely DRT 07K1, DRT 07K6 and DRT 07K15 developed through backcrossing were used along with the parent, bmr 3 source mutant (IS 21888) for evaluation of biohydrogen production. Results demonstrated that biohydrogen production varied amongst bmr derivatives under similar fermentation conditions. Significant negative correlation was observed between lignin content and fermentative biohydrogen production. All bmr derivatives with lower lignin content produced higher levels of biohydrogen compared to source bmr 3 (IS 21888) which has more lignin content. The maximum and a minimum biohydrogen production observed was 72 and 50 ml/g Total Volatile Solids (TVS) for the DRT 07K6 bmr3 derivative and bmr 3 (IS 21888) respectively. Acetate and butyrate were accounted >85% of volatile fatty acids, indicating acid type fermentations. Statistical analysis revealed that all bmr mutant derivatives with respect to source differ significantly in cumulative biohydrogen production, plant height, grain yield and lignin content. Biohydrogen production from biomass associated at least two different levels, one at lignin entanglement another at the polymeric nature of cellulose and hemicellulose. Further studies are necessary to determine the effect of biomass structure associated with different bmr traits on the microbial growth and biohydrogen production rate. Crown Copyright (C) 2011, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.

Keywords

  • Anaerobic fermentation, Biohydrogen, Brown midrib mutants, HYDROGEN-PRODUCTION, Microbial consortia, Sorghum stalks