Identification of the major proteins of an immune modulating fraction from adult Fasciola hepatica resleased by Nonident P40

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Identification of the major proteins of an immune modulating fraction from adult Fasciola hepatica resleased by Nonident P40. / Morphew, R. M.; Hamilton, Clare M.; Wright, H. A.; Dowling, David J.; O'Neill, Sandra M.; Brophy, P. M.

In: Veterinary Parasitology, Vol. 191, No. 3-4, 31.01.2013, p. 379–385.

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Morphew, R. M. ; Hamilton, Clare M. ; Wright, H. A. ; Dowling, David J. ; O'Neill, Sandra M. ; Brophy, P. M. / Identification of the major proteins of an immune modulating fraction from adult Fasciola hepatica resleased by Nonident P40. In: Veterinary Parasitology. 2013 ; Vol. 191, No. 3-4. pp. 379–385.

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@article{a1232ea9b06e401a80cc95db7e8e6f05,
title = "Identification of the major proteins of an immune modulating fraction from adult Fasciola hepatica resleased by Nonident P40",
abstract = "Fasciola hepatica NP-40 released protein extract (FhNPE) exhibits potent Th1 immunosuppressive properties in vitro and in vivo. However, the protein composition of this active fraction, responsible for Th1 immune modulatory activity, has yet to be resolved. Therefore, FhNPE, a Nonidet P-40 extract, was subjected to a proteomic analysis in order to identify individual protein components. This was performed using an in house F. hepatica EST database following 2D electrophoresis combined with de novo sequencing based mass spectrometry. The identified proteins, a mixture of excretory/secretory and membrane-associated proteins, are associated with stress response and chaperoning, energy metabolism and cytoskeletal components. The immune modulatory properties of these identified protein(s) are discussed and HSP70 from F. hepatica is highlighted as a potential host immune modulator for future study.",
keywords = "Anti-inflammatory, Heat shock protein, Proteomics",
author = "Morphew, {R. M.} and Hamilton, {Clare M.} and Wright, {H. A.} and Dowling, {David J.} and O'Neill, {Sandra M.} and Brophy, {P. M.}",
note = "Morphew, R. M., Hamilton, C. M., Wright, H. A., Dowling, D. J., O'Neill, S. M., Brophy, P. M. (2013). Identification of the major proteins of an immune modulating fraction from adult Fasciola hepatica resleased by Nonident P40. Veterinary Parasitology, 191 (3-4), 379–38 RONO: 00",
year = "2013",
month = "1",
day = "31",
doi = "10.1016/j.vetpar.2012.08.029",
language = "English",
volume = "191",
pages = "379–385",
journal = "Veterinary Parasitology",
issn = "0304-4017",
publisher = "Elsevier",
number = "3-4",

}

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TY - JOUR

T1 - Identification of the major proteins of an immune modulating fraction from adult Fasciola hepatica resleased by Nonident P40

AU - Morphew, R. M.

AU - Hamilton, Clare M.

AU - Wright, H. A.

AU - Dowling, David J.

AU - O'Neill, Sandra M.

AU - Brophy, P. M.

N1 - Morphew, R. M., Hamilton, C. M., Wright, H. A., Dowling, D. J., O'Neill, S. M., Brophy, P. M. (2013). Identification of the major proteins of an immune modulating fraction from adult Fasciola hepatica resleased by Nonident P40. Veterinary Parasitology, 191 (3-4), 379–38 RONO: 00

PY - 2013/1/31

Y1 - 2013/1/31

N2 - Fasciola hepatica NP-40 released protein extract (FhNPE) exhibits potent Th1 immunosuppressive properties in vitro and in vivo. However, the protein composition of this active fraction, responsible for Th1 immune modulatory activity, has yet to be resolved. Therefore, FhNPE, a Nonidet P-40 extract, was subjected to a proteomic analysis in order to identify individual protein components. This was performed using an in house F. hepatica EST database following 2D electrophoresis combined with de novo sequencing based mass spectrometry. The identified proteins, a mixture of excretory/secretory and membrane-associated proteins, are associated with stress response and chaperoning, energy metabolism and cytoskeletal components. The immune modulatory properties of these identified protein(s) are discussed and HSP70 from F. hepatica is highlighted as a potential host immune modulator for future study.

AB - Fasciola hepatica NP-40 released protein extract (FhNPE) exhibits potent Th1 immunosuppressive properties in vitro and in vivo. However, the protein composition of this active fraction, responsible for Th1 immune modulatory activity, has yet to be resolved. Therefore, FhNPE, a Nonidet P-40 extract, was subjected to a proteomic analysis in order to identify individual protein components. This was performed using an in house F. hepatica EST database following 2D electrophoresis combined with de novo sequencing based mass spectrometry. The identified proteins, a mixture of excretory/secretory and membrane-associated proteins, are associated with stress response and chaperoning, energy metabolism and cytoskeletal components. The immune modulatory properties of these identified protein(s) are discussed and HSP70 from F. hepatica is highlighted as a potential host immune modulator for future study.

KW - Anti-inflammatory

KW - Heat shock protein

KW - Proteomics

UR - http://hdl.handle.net/2160/14212

U2 - 10.1016/j.vetpar.2012.08.029

DO - 10.1016/j.vetpar.2012.08.029

M3 - Article

VL - 191

SP - 379

EP - 385

JO - Veterinary Parasitology

JF - Veterinary Parasitology

SN - 0304-4017

IS - 3-4

ER -

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