Identification of the major proteins of an immune modulating fraction from adult Fasciola hepatica resleased by Nonident P40

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Type Article
Original languageEnglish
Pages (from-to)379–385
JournalVeterinary Parasitology
Volume191
Issue number3-4
Early online date11 Sep 2012
DOI
Publication statusPublished - 31 Jan 2013
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Abstract

Fasciola hepatica NP-40 released protein extract (FhNPE) exhibits potent Th1 immunosuppressive properties in vitro and in vivo. However, the protein composition of this active fraction, responsible for Th1 immune modulatory activity, has yet to be resolved. Therefore, FhNPE, a Nonidet P-40 extract, was subjected to a proteomic analysis in order to identify individual protein components. This was performed using an in house F. hepatica EST database following 2D electrophoresis combined with de novo sequencing based mass spectrometry. The identified proteins, a mixture of excretory/secretory and membrane-associated proteins, are associated with stress response and chaperoning, energy metabolism and cytoskeletal components. The immune modulatory properties of these identified protein(s) are discussed and HSP70 from F. hepatica is highlighted as a potential host immune modulator for future study.

Keywords

  • Anti-inflammatory, Heat shock protein, Proteomics

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