Identification of SNps for molecular breeding in Lolium perenne

Standard

Identification of SNps for molecular breeding in Lolium perenne. / Farrar, K.; Thomas, A. M.; Gallagher, J. A.; Armstead, Ian; Turner, Lesley; Humphreys, M. O.; Donnison, Iain.

2007. Poster session presented at Plant and Animal Genome XV Conference, San Diego, California, United States of America.

Research output: Contribution to conferencePoster

Harvard

Farrar, K, Thomas, AM, Gallagher, JA, Armstead, I, Turner, L, Humphreys, MO & Donnison, I 2007, 'Identification of SNps for molecular breeding in Lolium perenne', Plant and Animal Genome XV Conference, San Diego, California, United States of America, 13 Jan 2007 - 17 Jan 2007.

APA

Farrar, K., Thomas, A. M., Gallagher, J. A., Armstead, I., Turner, L., Humphreys, M. O., & Donnison, I. (2007). Identification of SNps for molecular breeding in Lolium perenne. Poster session presented at Plant and Animal Genome XV Conference, San Diego, California, United States of America.

Vancouver

Farrar K, Thomas AM, Gallagher JA, Armstead I, Turner L, Humphreys MO et al. Identification of SNps for molecular breeding in Lolium perenne. 2007. Poster session presented at Plant and Animal Genome XV Conference, San Diego, California, United States of America.

Author

Farrar, K. ; Thomas, A. M. ; Gallagher, J. A. ; Armstead, Ian ; Turner, Lesley ; Humphreys, M. O. ; Donnison, Iain. / Identification of SNps for molecular breeding in Lolium perenne. Poster session presented at Plant and Animal Genome XV Conference, San Diego, California, United States of America.

Bibtex - Download

@conference{59f2da37f4744b6280d56841bd864060,
title = "Identification of SNps for molecular breeding in Lolium perenne",
abstract = "This work is part of the EU Framework 5 project, GRASP (http://www.grasp-euv.dk/). Breeding programs rely on allelic variation between genotypes. In order to exploit this natural variation, genetic markers such as single nucleotide polymorphisms (SNPs) can be identified to enable accelerated PCR-based selection of suitable individuals for breeding. Many of the important traits which are targets for perennial ryegrass plant breeding are quantitatively inherited with a number of QTL underlying each trait. The most reliable and straightforward approach to clone genes underlying such QTL is by map-based cloning. It is therefore important to have the resources available to enable this, including the generation of large insert DNA libraries. Candidate genes for forage quality, nitrogen stability, flowering time, and sugar metabolism have been isolated using a Lolium perenne Bacterial Artificial Chromosome (BAC) library. Full length sequences including promoter and intron sequence have been obtained and primers designed to specific regions. These regions have then been amplified from 20 different L. perenne genotypes and analysed for allele specific SNPs. These SNPs will be tracked in genetically diverse L. perenne populations undergoing a range of selection pressures in order to assess any shifts in allele frequency. The L. perenne BAC library generated compliments an existing BAC library of the closely related species Festuca pratensis, and will facilitate the production of physical maps, genome sequencing, marker development and map based cloning strategies",
author = "K. Farrar and Thomas, {A. M.} and Gallagher, {J. A.} and Ian Armstead and Lesley Turner and Humphreys, {M. O.} and Iain Donnison",
note = "This work is part of the EU Framework 5 project, GRASP-QLK5-2002-00862; Plant and Animal Genome XV Conference ; Conference date: 13-01-2007 Through 17-01-2007",
year = "2007",
language = "English",

}

RIS (suitable for import to EndNote) - Download

TY - CONF

T1 - Identification of SNps for molecular breeding in Lolium perenne

AU - Farrar, K.

AU - Thomas, A. M.

AU - Gallagher, J. A.

AU - Armstead, Ian

AU - Turner, Lesley

AU - Humphreys, M. O.

AU - Donnison, Iain

N1 - This work is part of the EU Framework 5 project, GRASP-QLK5-2002-00862

PY - 2007

Y1 - 2007

N2 - This work is part of the EU Framework 5 project, GRASP (http://www.grasp-euv.dk/). Breeding programs rely on allelic variation between genotypes. In order to exploit this natural variation, genetic markers such as single nucleotide polymorphisms (SNPs) can be identified to enable accelerated PCR-based selection of suitable individuals for breeding. Many of the important traits which are targets for perennial ryegrass plant breeding are quantitatively inherited with a number of QTL underlying each trait. The most reliable and straightforward approach to clone genes underlying such QTL is by map-based cloning. It is therefore important to have the resources available to enable this, including the generation of large insert DNA libraries. Candidate genes for forage quality, nitrogen stability, flowering time, and sugar metabolism have been isolated using a Lolium perenne Bacterial Artificial Chromosome (BAC) library. Full length sequences including promoter and intron sequence have been obtained and primers designed to specific regions. These regions have then been amplified from 20 different L. perenne genotypes and analysed for allele specific SNPs. These SNPs will be tracked in genetically diverse L. perenne populations undergoing a range of selection pressures in order to assess any shifts in allele frequency. The L. perenne BAC library generated compliments an existing BAC library of the closely related species Festuca pratensis, and will facilitate the production of physical maps, genome sequencing, marker development and map based cloning strategies

AB - This work is part of the EU Framework 5 project, GRASP (http://www.grasp-euv.dk/). Breeding programs rely on allelic variation between genotypes. In order to exploit this natural variation, genetic markers such as single nucleotide polymorphisms (SNPs) can be identified to enable accelerated PCR-based selection of suitable individuals for breeding. Many of the important traits which are targets for perennial ryegrass plant breeding are quantitatively inherited with a number of QTL underlying each trait. The most reliable and straightforward approach to clone genes underlying such QTL is by map-based cloning. It is therefore important to have the resources available to enable this, including the generation of large insert DNA libraries. Candidate genes for forage quality, nitrogen stability, flowering time, and sugar metabolism have been isolated using a Lolium perenne Bacterial Artificial Chromosome (BAC) library. Full length sequences including promoter and intron sequence have been obtained and primers designed to specific regions. These regions have then been amplified from 20 different L. perenne genotypes and analysed for allele specific SNPs. These SNPs will be tracked in genetically diverse L. perenne populations undergoing a range of selection pressures in order to assess any shifts in allele frequency. The L. perenne BAC library generated compliments an existing BAC library of the closely related species Festuca pratensis, and will facilitate the production of physical maps, genome sequencing, marker development and map based cloning strategies

UR - http://hdl.handle.net/2160/43692

M3 - Poster

T2 - Plant and Animal Genome XV Conference

Y2 - 13 January 2007 through 17 January 2007

ER -

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