Identification and pathogenicity of Fusarium species associated with sesame (Sesamum indicum L.) seeds from the Punjab, Pakistan

Authors Organisations
  • Brian Gagosh Nayyar(Author)
    Pir Mehr Ali Shah Arid Agriculture University
  • Steve Woodward(Author)
    University of Aberdeen
  • Luis Mur(Author)
  • Abida Akram(Author)
    Pir Mehr Ali Shah Arid Agriculture University
  • Muhammad Arshad(Author)
    Pir Mehr Ali Shah Arid Agriculture University
  • S. M. Saqlan Naqvi(Author)
    Pir Mehr Ali Shah Arid Agriculture University
  • Shaista Akhund(Author)
    Pir Mehr Ali Shah Arid Agriculture University
Type Article
Original languageEnglish
Pages (from-to)128-135
Number of pages8
JournalPhysiological and Molecular Plant Pathology
Volume102
Early online date03 Feb 2018
DOI
Publication statusPublished - 01 Apr 2018
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Abstract

Sesame (Sesamum indicum) is an oil-crop in Asia and Africa and is widely grown in the Punjab region of Pakistan. A total of 105 sesame seed samples were collected from different locations in the Punjab from which 520 isolates of Fusarium spp. were recovered. These isolates were initially grouped and identified based on morphological characteristics. The identities of representatives of the three most frequently isolated groups (strains designated F01, F98, F153) were identified as Fusarium proliferatum, on the basis of the sequencing of ITS of rDNA and translation elongation factor (TEF-1α) gene regions. Phylogenetic trees generated using the maximum likelihood method showed that these three isolates and a F. proliferatum reference sequence grouped in the same clade with F. phyllophilum, the most closely related species. Pathogenicity tests demonstrated that these three isolates caused disease on sesame plants. Disease Incidence (DI) and Disease Severity Index (DSI) data indicated that F01 was the most virulent isolate, with DI and DSI of approximately 70%. Culture filtrates of F01 reduced sesame seed germination (to 40%) and vigor (to 16.5%) of sesame seedlings. This baseline study suggests that F. proliferatum infection of sesame seeds could be a major source of yield loss in the Punjab, Pakistan which requires further attention

Keywords

  • sesame, Fusarium, blotter paper method, ITS, TEF-1α