Genetic Transformation of Protoplasts Isolated from Leave of Lolium temulentum and Lolium perenne

Standard

Genetic Transformation of Protoplasts Isolated from Leave of Lolium temulentum and Lolium perenne. / Davies, Huw R.; Maddison, Anne; Phillips, Dylan; Jones, Huw.

Cereal Genomics. ed. / Luis M. Vaschetto. Springer Nature, 2019. (Methods in Molecular Biology; Vol. 2072).

Research output: Chapter in Book/Report/Conference proceedingChapter

Harvard

Davies, HR, Maddison, A, Phillips, D & Jones, H 2019, Genetic Transformation of Protoplasts Isolated from Leave of Lolium temulentum and Lolium perenne. in LM Vaschetto (ed.), Cereal Genomics. Methods in Molecular Biology, vol. 2072, Springer Nature. https://doi.org/10.1007/978-1-4939-9865-4

APA

Davies, H. R., Maddison, A., Phillips, D., & Jones, H. (2019). Genetic Transformation of Protoplasts Isolated from Leave of Lolium temulentum and Lolium perenne. In L. M. Vaschetto (Ed.), Cereal Genomics (Methods in Molecular Biology; Vol. 2072). Springer Nature. https://doi.org/10.1007/978-1-4939-9865-4

Vancouver

Author

Davies, Huw R. ; Maddison, Anne ; Phillips, Dylan ; Jones, Huw. / Genetic Transformation of Protoplasts Isolated from Leave of Lolium temulentum and Lolium perenne. Cereal Genomics. editor / Luis M. Vaschetto. Springer Nature, 2019. (Methods in Molecular Biology).

Bibtex - Download

@inbook{afab1d66437b4ade871c3b13b5ec71ee,
title = "Genetic Transformation of Protoplasts Isolated from Leave of Lolium temulentum and Lolium perenne",
abstract = "Transient expression of inserted recombinant DNA in plant protoplasts is a widely used tool for functional genomics research. Recently it has been utilized to screen potential sgRNA guides for CRISPR-mediated genome editing. However, little research has been conducted into the use of transient expression of protoplasts in Lolium perenne (a globally important pasture, hay, and amenity grass), and no studies have been conducted into Lolium temulentum (a weed in cereal crops but a potentially useful model species for Lolium research). In this chapter, we describe a methodology of protoplast extraction and transformation from 14-day-old leaf mesophyll cells from L. perenne and L. temulentum. We believe this is the first report of a procedure for obtaining high density, viable protoplasts from L. temulentum. The method of polyethylene glycol (PEG)-mediated transformation is also described to achieve genetic transformation of protoplasts",
keywords = "protoplasts, lolium temulentum, lolium perenne, transient expression, PEG-mediated transformation",
author = "Davies, {Huw R.} and Anne Maddison and Dylan Phillips and Huw Jones",
year = "2019",
month = "9",
day = "21",
doi = "10.1007/978-1-4939-9865-4",
language = "English",
isbn = "978-1-4939-9864-7",
series = "Methods in Molecular Biology",
publisher = "Springer Nature",
editor = "Vaschetto, {Luis M.}",
booktitle = "Cereal Genomics",
address = "Switzerland",

}

RIS (suitable for import to EndNote) - Download

TY - CHAP

T1 - Genetic Transformation of Protoplasts Isolated from Leave of Lolium temulentum and Lolium perenne

AU - Davies, Huw R.

AU - Maddison, Anne

AU - Phillips, Dylan

AU - Jones, Huw

PY - 2019/9/21

Y1 - 2019/9/21

N2 - Transient expression of inserted recombinant DNA in plant protoplasts is a widely used tool for functional genomics research. Recently it has been utilized to screen potential sgRNA guides for CRISPR-mediated genome editing. However, little research has been conducted into the use of transient expression of protoplasts in Lolium perenne (a globally important pasture, hay, and amenity grass), and no studies have been conducted into Lolium temulentum (a weed in cereal crops but a potentially useful model species for Lolium research). In this chapter, we describe a methodology of protoplast extraction and transformation from 14-day-old leaf mesophyll cells from L. perenne and L. temulentum. We believe this is the first report of a procedure for obtaining high density, viable protoplasts from L. temulentum. The method of polyethylene glycol (PEG)-mediated transformation is also described to achieve genetic transformation of protoplasts

AB - Transient expression of inserted recombinant DNA in plant protoplasts is a widely used tool for functional genomics research. Recently it has been utilized to screen potential sgRNA guides for CRISPR-mediated genome editing. However, little research has been conducted into the use of transient expression of protoplasts in Lolium perenne (a globally important pasture, hay, and amenity grass), and no studies have been conducted into Lolium temulentum (a weed in cereal crops but a potentially useful model species for Lolium research). In this chapter, we describe a methodology of protoplast extraction and transformation from 14-day-old leaf mesophyll cells from L. perenne and L. temulentum. We believe this is the first report of a procedure for obtaining high density, viable protoplasts from L. temulentum. The method of polyethylene glycol (PEG)-mediated transformation is also described to achieve genetic transformation of protoplasts

KW - protoplasts

KW - lolium temulentum

KW - lolium perenne

KW - transient expression

KW - PEG-mediated transformation

U2 - 10.1007/978-1-4939-9865-4

DO - 10.1007/978-1-4939-9865-4

M3 - Chapter

SN - 978-1-4939-9864-7

T3 - Methods in Molecular Biology

BT - Cereal Genomics

A2 - Vaschetto, Luis M.

PB - Springer Nature

ER -

View graph of relations
Citation formats