Forage type and fish oil cause shifts in rumen bacterial diversity

Sharon A. Huws; Michael R. F. Lee; Stefan M. Muetzel; Mark B. Scott; R. John Wallace; Nigel D. Scollan

doi:10.1111/j.1574-6941.2010.00892.x

Forage type and fish oil cause shifts in rumen bacterial diversity

Standard

Forage type and fish oil cause shifts in rumen bacterial diversity. / Huws, Sharon A.; Lee, Michael R. F.; Muetzel, Stefan M.; Scott, Mark B.; Wallace, R. John; Scollan, Nigel D.

In: FEMS Microbiology Ecology, Vol. 73, No. 2, 01.08.2010, p. 396-407.

Research output: Contribution to journal › Article › peer-review

Harvard

Huws, SA, Lee, MRF, Muetzel, SM, Scott, MB, Wallace, RJ & Scollan, ND 2010, 'Forage type and fish oil cause shifts in rumen bacterial diversity', FEMS Microbiology Ecology, vol. 73, no. 2, pp. 396-407. https://doi.org/10.1111/j.1574-6941.2010.00892.x

APA

Huws, S. A., Lee, M. R. F., Muetzel, S. M., Scott, M. B., Wallace, R. J., & Scollan, N. D. (2010). Forage type and fish oil cause shifts in rumen bacterial diversity. FEMS Microbiology Ecology, 73(2), 396-407. https://doi.org/10.1111/j.1574-6941.2010.00892.x

Vancouver

Huws SA, Lee MRF, Muetzel SM, Scott MB, Wallace RJ, Scollan ND. Forage type and fish oil cause shifts in rumen bacterial diversity. FEMS Microbiology Ecology. 2010 Aug 1;73(2):396-407. https://doi.org/10.1111/j.1574-6941.2010.00892.x

Author

Huws, Sharon A. ; Lee, Michael R. F. ; Muetzel, Stefan M. ; Scott, Mark B. ; Wallace, R. John ; Scollan, Nigel D. / Forage type and fish oil cause shifts in rumen bacterial diversity. In: FEMS Microbiology Ecology. 2010 ; Vol. 73, No. 2. pp. 396-407.

Bibtex - Download

@article{1d4204176db444728d4e09986858354d,

title = "Forage type and fish oil cause shifts in rumen bacterial diversity",

abstract = "Despite evidence supporting improved incorporation of beneficial polyunsaturated fatty acids (PUFA) into ruminant products, such as meat and milk, following red clover and fish oil (FO) inclusion in the ruminant diet, little is known regarding the concomitant bacterial diversity. We evaluated the effects of feeding grass vs. red clover silage with incremental FO inclusion on known lipolytic, biohydrogenating, cellulolytic and proteolytic rumen bacterial communities of steers. Following 14 days of dietary adaptation, liquid-associated (LAB) and solid-associated (SAB) bacterial communities were harvested, DNA extracted and bacterial denaturing gradient gel electrophoresis (DGGE) and specific-bacterial quantitative PCR (QPCR) were undertaken. DGGE-derived dendrograms showed that diet caused the greatest change in LAB and SAB bacterial diversity, with FO inclusion at the 2% and 3% dry matter intake also causing some changes. QPCR revealed that diet resulted in changes in the DNA concentration of Anaerovibrio lipolytica, the Butyrivibrio proteoclasticus group, Fibrobacter succinogenes, Ruminococcus albus and Ruminococcus flavefaciens. FO inclusion caused changes in A. lipolytica, F. succinogenes and R. flavefaciens DNA concentration only. In the B. proteoclasticus group, which are the only known bacteria with the capacity to biohydrogenate PUFA to 18:0, DNA concentration did not correlate to 18:0 flow to the duodenum, however, suggesting that other bacteria may play a role in biohydrogenation. A greater understanding of microbial changes that accompany beneficial dietary changes will lead to novel strategies to improve ruminant product quality.",

keywords = "red clover, grass, fish oil, biohydrogenation, bacteria, rumen",

author = "Huws, {Sharon A.} and Lee, {Michael R. F.} and Muetzel, {Stefan M.} and Scott, {Mark B.} and Wallace, {R. John} and Scollan, {Nigel D.}",

note = "Huws, S. A., Lee, M. R. F., Muetzel, S. M., Scott, M. B., Wallace, R. J., Scollan, N. D. (2010). Forage type and fish oil cause shifts in rumen bacterial diversity. FEMS Microbiology Ecology, 73, (2), 396-407. IMPF: 03.45 RONO: 06746 Sponsorship: Objective One European Social Fund (ESF)",

year = "2010",

month = aug,

day = "1",

doi = "10.1111/j.1574-6941.2010.00892.x",

language = "English",

volume = "73",

pages = "396--407",

journal = "FEMS Microbiology Ecology",

issn = "0168-6496",

publisher = "Wiley",

number = "2",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Forage type and fish oil cause shifts in rumen bacterial diversity

AU - Huws, Sharon A.

AU - Lee, Michael R. F.

AU - Muetzel, Stefan M.

AU - Scott, Mark B.

AU - Wallace, R. John

AU - Scollan, Nigel D.

N1 - Huws, S. A., Lee, M. R. F., Muetzel, S. M., Scott, M. B., Wallace, R. J., Scollan, N. D. (2010). Forage type and fish oil cause shifts in rumen bacterial diversity. FEMS Microbiology Ecology, 73, (2), 396-407. IMPF: 03.45 RONO: 06746 Sponsorship: Objective One European Social Fund (ESF)

PY - 2010/8/1

Y1 - 2010/8/1

N2 - Despite evidence supporting improved incorporation of beneficial polyunsaturated fatty acids (PUFA) into ruminant products, such as meat and milk, following red clover and fish oil (FO) inclusion in the ruminant diet, little is known regarding the concomitant bacterial diversity. We evaluated the effects of feeding grass vs. red clover silage with incremental FO inclusion on known lipolytic, biohydrogenating, cellulolytic and proteolytic rumen bacterial communities of steers. Following 14 days of dietary adaptation, liquid-associated (LAB) and solid-associated (SAB) bacterial communities were harvested, DNA extracted and bacterial denaturing gradient gel electrophoresis (DGGE) and specific-bacterial quantitative PCR (QPCR) were undertaken. DGGE-derived dendrograms showed that diet caused the greatest change in LAB and SAB bacterial diversity, with FO inclusion at the 2% and 3% dry matter intake also causing some changes. QPCR revealed that diet resulted in changes in the DNA concentration of Anaerovibrio lipolytica, the Butyrivibrio proteoclasticus group, Fibrobacter succinogenes, Ruminococcus albus and Ruminococcus flavefaciens. FO inclusion caused changes in A. lipolytica, F. succinogenes and R. flavefaciens DNA concentration only. In the B. proteoclasticus group, which are the only known bacteria with the capacity to biohydrogenate PUFA to 18:0, DNA concentration did not correlate to 18:0 flow to the duodenum, however, suggesting that other bacteria may play a role in biohydrogenation. A greater understanding of microbial changes that accompany beneficial dietary changes will lead to novel strategies to improve ruminant product quality.

AB - Despite evidence supporting improved incorporation of beneficial polyunsaturated fatty acids (PUFA) into ruminant products, such as meat and milk, following red clover and fish oil (FO) inclusion in the ruminant diet, little is known regarding the concomitant bacterial diversity. We evaluated the effects of feeding grass vs. red clover silage with incremental FO inclusion on known lipolytic, biohydrogenating, cellulolytic and proteolytic rumen bacterial communities of steers. Following 14 days of dietary adaptation, liquid-associated (LAB) and solid-associated (SAB) bacterial communities were harvested, DNA extracted and bacterial denaturing gradient gel electrophoresis (DGGE) and specific-bacterial quantitative PCR (QPCR) were undertaken. DGGE-derived dendrograms showed that diet caused the greatest change in LAB and SAB bacterial diversity, with FO inclusion at the 2% and 3% dry matter intake also causing some changes. QPCR revealed that diet resulted in changes in the DNA concentration of Anaerovibrio lipolytica, the Butyrivibrio proteoclasticus group, Fibrobacter succinogenes, Ruminococcus albus and Ruminococcus flavefaciens. FO inclusion caused changes in A. lipolytica, F. succinogenes and R. flavefaciens DNA concentration only. In the B. proteoclasticus group, which are the only known bacteria with the capacity to biohydrogenate PUFA to 18:0, DNA concentration did not correlate to 18:0 flow to the duodenum, however, suggesting that other bacteria may play a role in biohydrogenation. A greater understanding of microbial changes that accompany beneficial dietary changes will lead to novel strategies to improve ruminant product quality.

KW - red clover

KW - grass

KW - fish oil

KW - biohydrogenation

KW - bacteria

KW - rumen

U2 - 10.1111/j.1574-6941.2010.00892.x

DO - 10.1111/j.1574-6941.2010.00892.x

M3 - Article

C2 - 20491929

VL - 73

SP - 396