Expression of FlHMA3, a P
            1B2-ATPase from Festulolium loliaceum, correlates with response to cadmium stress

Qiang Guo; Lin Meng; Mike W. Humphreys; John Scullion; Luis A.J. Mur

doi:10.1016/j.plaphy.2017.01.013

Expression of FlHMA3, a P _1B2-ATPase from Festulolium loliaceum, correlates with response to cadmium stress

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Expression of FlHMA3, a P _1B2-ATPase from Festulolium loliaceum, correlates with response to cadmium stress. / Guo, Qiang; Meng, Lin; Humphreys, Mike W. et al.

In: Plant Physiology and Biochemistry, Vol. 112, 01.03.2017, p. 270-277.

Research output: Contribution to journal › Article › peer-review

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@article{d594c6e6efa5441fbc60f0c0b31af0d4,

title = "Expression of FlHMA3, a P 1B2-ATPase from Festulolium loliaceum, correlates with response to cadmium stress",

abstract = "Heavy metal ATPase 3 (HMA3), a P1B2-ATPase, is a key tonoplast transporter involved in mediating the vacuolar sequestration of cadmium (Cd) to detoxify the intake of this element by plants. HMA3 expression in response to Cd stress has not been previously examined in the grass hybrid species Festulolium loliaceum (Huds.) P. Fourn. In this study, FlHMA3 isolated from F. loliaceum was found to comprise 833 amino acid residues with 77% homology to the rice OsHMA3. Transient expression of FlHMA3 fused to enhanced green fluorescent protein in Arabidopsis protoplasts suggested its localization to vacuolar membranes. Quantitative real-time RT-PCR analysis of F. loliaceum revealed that FlHMA3 is expressed predominantly within roots and up-regulated by excess Cd. Over the 168 h treatment, Cd content of F. loliaceum roots was significantly higher than that of shoots, regardless of external CdCl2 concentrations. A significant positive correlation was found between FlHMA3 expression and Cd accumulation in roots of F. loliaceum seedlings subjected to 10–100 mg L-1 CdCl2 for 168 h or, in a separate experiment, to 25 or 100 mg L-1 CdCl2 for the same duration. These findings provide evidence that FlHMA3 encodes a vacuolar P1B2-ATPase that may play an important role in Cd2+ sequestration into root cell vacuoles, thereby limiting the entry of Cd2+ into the cytoplasm and reducing Cd2+ toxicity.",

keywords = "Cadmium, Festulolium loliaceum, FlHMA3, Phytomediation, Vacuole sequestration",

author = "Qiang Guo and Lin Meng and Humphreys, {Mike W.} and John Scullion and Mur, {Luis A.J.}",

note = "Funding Information: This research was financially supported by the International Cooperation Project (grant number GJHZ2015-1), the Institute for Science and Technology Innovation Team Project (grant number JNKST201604), the Scientific Innovation Ability Construction Project (grant number KJCX20140103) of the Beijing Academy of Agriculture and Forestry Sciences, and the Beijing Natural Science Foundation (grant number 6152008). Collaborations with IBERS, Aberystwyth University, were achieved through a memorandum of understanding and a material transfer agreement brought about following a Biotechnology and Biological Sciences Research Council International Partnering Award (BB/M027945/1). We are also grateful to the anonymous reviewers for their comments on the initial version of the manuscript. Publisher Copyright: {\textcopyright} 2017 Elsevier Masson SAS",

year = "2017",

month = mar,

day = "1",

doi = "10.1016/j.plaphy.2017.01.013",

language = "English",

volume = "112",

pages = "270--277",

journal = "Plant Physiology and Biochemistry",

issn = "0981-9428",

publisher = "Elsevier",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Expression of FlHMA3, a P 1B2-ATPase from Festulolium loliaceum, correlates with response to cadmium stress

AU - Guo, Qiang

AU - Meng, Lin

AU - Humphreys, Mike W.

AU - Scullion, John

AU - Mur, Luis A.J.

N1 - Funding Information: This research was financially supported by the International Cooperation Project (grant number GJHZ2015-1), the Institute for Science and Technology Innovation Team Project (grant number JNKST201604), the Scientific Innovation Ability Construction Project (grant number KJCX20140103) of the Beijing Academy of Agriculture and Forestry Sciences, and the Beijing Natural Science Foundation (grant number 6152008). Collaborations with IBERS, Aberystwyth University, were achieved through a memorandum of understanding and a material transfer agreement brought about following a Biotechnology and Biological Sciences Research Council International Partnering Award (BB/M027945/1). We are also grateful to the anonymous reviewers for their comments on the initial version of the manuscript. Publisher Copyright: © 2017 Elsevier Masson SAS

PY - 2017/3/1

Y1 - 2017/3/1

N2 - Heavy metal ATPase 3 (HMA3), a P1B2-ATPase, is a key tonoplast transporter involved in mediating the vacuolar sequestration of cadmium (Cd) to detoxify the intake of this element by plants. HMA3 expression in response to Cd stress has not been previously examined in the grass hybrid species Festulolium loliaceum (Huds.) P. Fourn. In this study, FlHMA3 isolated from F. loliaceum was found to comprise 833 amino acid residues with 77% homology to the rice OsHMA3. Transient expression of FlHMA3 fused to enhanced green fluorescent protein in Arabidopsis protoplasts suggested its localization to vacuolar membranes. Quantitative real-time RT-PCR analysis of F. loliaceum revealed that FlHMA3 is expressed predominantly within roots and up-regulated by excess Cd. Over the 168 h treatment, Cd content of F. loliaceum roots was significantly higher than that of shoots, regardless of external CdCl2 concentrations. A significant positive correlation was found between FlHMA3 expression and Cd accumulation in roots of F. loliaceum seedlings subjected to 10–100 mg L-1 CdCl2 for 168 h or, in a separate experiment, to 25 or 100 mg L-1 CdCl2 for the same duration. These findings provide evidence that FlHMA3 encodes a vacuolar P1B2-ATPase that may play an important role in Cd2+ sequestration into root cell vacuoles, thereby limiting the entry of Cd2+ into the cytoplasm and reducing Cd2+ toxicity.

AB - Heavy metal ATPase 3 (HMA3), a P1B2-ATPase, is a key tonoplast transporter involved in mediating the vacuolar sequestration of cadmium (Cd) to detoxify the intake of this element by plants. HMA3 expression in response to Cd stress has not been previously examined in the grass hybrid species Festulolium loliaceum (Huds.) P. Fourn. In this study, FlHMA3 isolated from F. loliaceum was found to comprise 833 amino acid residues with 77% homology to the rice OsHMA3. Transient expression of FlHMA3 fused to enhanced green fluorescent protein in Arabidopsis protoplasts suggested its localization to vacuolar membranes. Quantitative real-time RT-PCR analysis of F. loliaceum revealed that FlHMA3 is expressed predominantly within roots and up-regulated by excess Cd. Over the 168 h treatment, Cd content of F. loliaceum roots was significantly higher than that of shoots, regardless of external CdCl2 concentrations. A significant positive correlation was found between FlHMA3 expression and Cd accumulation in roots of F. loliaceum seedlings subjected to 10–100 mg L-1 CdCl2 for 168 h or, in a separate experiment, to 25 or 100 mg L-1 CdCl2 for the same duration. These findings provide evidence that FlHMA3 encodes a vacuolar P1B2-ATPase that may play an important role in Cd2+ sequestration into root cell vacuoles, thereby limiting the entry of Cd2+ into the cytoplasm and reducing Cd2+ toxicity.

KW - Cadmium

KW - Festulolium loliaceum

KW - FlHMA3

KW - Phytomediation

KW - Vacuole sequestration

UR - http://hdl.handle.net/2160/44512

UR - http://www.scopus.com/inward/record.url?scp=85010000352&partnerID=8YFLogxK

U2 - 10.1016/j.plaphy.2017.01.013

DO - 10.1016/j.plaphy.2017.01.013

M3 - Article

C2 - 28113076

VL - 112