Evolutionary history expands the range of signaling interactions in hybrid multikinase networks

Standard

Evolutionary history expands the range of signaling interactions in hybrid multikinase networks. / Ortet, Philippe; Fochesato, Sylvain; Bitbol, Anne-Florence; Whitworth, David E.; Lalaouna, David; Santaella, Catherine; Heulin, Thierry; Achouak, Wafa; Barakat, Mohamed.

In: Scientific Reports, Vol. 11, No. 1, 03.06.2021, p. 11763.

Research output: Contribution to journalArticlepeer-review

Harvard

Ortet, P, Fochesato, S, Bitbol, A-F, Whitworth, DE, Lalaouna, D, Santaella, C, Heulin, T, Achouak, W & Barakat, M 2021, 'Evolutionary history expands the range of signaling interactions in hybrid multikinase networks', Scientific Reports, vol. 11, no. 1, pp. 11763. https://doi.org/10.1038/s41598-021-91260-w

APA

Ortet, P., Fochesato, S., Bitbol, A-F., Whitworth, D. E., Lalaouna, D., Santaella, C., Heulin, T., Achouak, W., & Barakat, M. (2021). Evolutionary history expands the range of signaling interactions in hybrid multikinase networks. Scientific Reports, 11(1), 11763. https://doi.org/10.1038/s41598-021-91260-w

Vancouver

Ortet P, Fochesato S, Bitbol A-F, Whitworth DE, Lalaouna D, Santaella C et al. Evolutionary history expands the range of signaling interactions in hybrid multikinase networks. Scientific Reports. 2021 Jun 3;11(1):11763. https://doi.org/10.1038/s41598-021-91260-w

Author

Ortet, Philippe ; Fochesato, Sylvain ; Bitbol, Anne-Florence ; Whitworth, David E. ; Lalaouna, David ; Santaella, Catherine ; Heulin, Thierry ; Achouak, Wafa ; Barakat, Mohamed. / Evolutionary history expands the range of signaling interactions in hybrid multikinase networks. In: Scientific Reports. 2021 ; Vol. 11, No. 1. pp. 11763.

Bibtex - Download

@article{b3887b4ac88a4b60a37c5fe58427a72f,
title = "Evolutionary history expands the range of signaling interactions in hybrid multikinase networks",
abstract = "Abstract: Two-component systems (TCSs) are ubiquitous signaling pathways, typically comprising a sensory histidine kinase (HK) and a response regulator, which communicate via intermolecular kinase-to-receiver domain phosphotransfer. Hybrid HKs constitute non-canonical TCS signaling pathways, with transmitter and receiver domains within a single protein communicating via intramolecular phosphotransfer. Here, we report how evolutionary relationships between hybrid HKs can be used as predictors of potential intermolecular and intramolecular interactions ({\textquoteleft}phylogenetic promiscuity{\textquoteright}). We used domain-swap genes chimeras to investigate the specificity of phosphotransfer within hybrid HKs of the GacS–GacA multikinase network of Pseudomonas brassicacearum. The receiver domain of GacS was replaced with those from nine donor hybrid HKs. Three chimeras with receivers from other hybrid HKs demonstrated correct functioning through complementation of a gacS mutant, which was dependent on strains having a functional gacA. Formation of functional chimeras was predictable on the basis of evolutionary heritage, and raises the possibility that HKs sharing a common ancestor with GacS might remain components of the contemporary GacS network. The results also demonstrate that understanding the evolutionary heritage of signaling domains in sophisticated networks allows their rational rewiring by simple domain transplantation, with implications for the creation of designer networks and inference of functional interactions.",
keywords = "Article, /631/326, /631/326/41, /631/326/41/2529, /631/114, /631/114/2114, article",
author = "Philippe Ortet and Sylvain Fochesato and Anne-Florence Bitbol and Whitworth, {David E.} and David Lalaouna and Catherine Santaella and Thierry Heulin and Wafa Achouak and Mohamed Barakat",
note = "We thank all the members of the LEMiRE team and Ned S. Wingreen for valuable and stimulating discussions.",
year = "2021",
month = jun,
day = "3",
doi = "10.1038/s41598-021-91260-w",
language = "English",
volume = "11",
pages = "11763",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Springer Nature",
number = "1",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Evolutionary history expands the range of signaling interactions in hybrid multikinase networks

AU - Ortet, Philippe

AU - Fochesato, Sylvain

AU - Bitbol, Anne-Florence

AU - Whitworth, David E.

AU - Lalaouna, David

AU - Santaella, Catherine

AU - Heulin, Thierry

AU - Achouak, Wafa

AU - Barakat, Mohamed

N1 - We thank all the members of the LEMiRE team and Ned S. Wingreen for valuable and stimulating discussions.

PY - 2021/6/3

Y1 - 2021/6/3

N2 - Abstract: Two-component systems (TCSs) are ubiquitous signaling pathways, typically comprising a sensory histidine kinase (HK) and a response regulator, which communicate via intermolecular kinase-to-receiver domain phosphotransfer. Hybrid HKs constitute non-canonical TCS signaling pathways, with transmitter and receiver domains within a single protein communicating via intramolecular phosphotransfer. Here, we report how evolutionary relationships between hybrid HKs can be used as predictors of potential intermolecular and intramolecular interactions (‘phylogenetic promiscuity’). We used domain-swap genes chimeras to investigate the specificity of phosphotransfer within hybrid HKs of the GacS–GacA multikinase network of Pseudomonas brassicacearum. The receiver domain of GacS was replaced with those from nine donor hybrid HKs. Three chimeras with receivers from other hybrid HKs demonstrated correct functioning through complementation of a gacS mutant, which was dependent on strains having a functional gacA. Formation of functional chimeras was predictable on the basis of evolutionary heritage, and raises the possibility that HKs sharing a common ancestor with GacS might remain components of the contemporary GacS network. The results also demonstrate that understanding the evolutionary heritage of signaling domains in sophisticated networks allows their rational rewiring by simple domain transplantation, with implications for the creation of designer networks and inference of functional interactions.

AB - Abstract: Two-component systems (TCSs) are ubiquitous signaling pathways, typically comprising a sensory histidine kinase (HK) and a response regulator, which communicate via intermolecular kinase-to-receiver domain phosphotransfer. Hybrid HKs constitute non-canonical TCS signaling pathways, with transmitter and receiver domains within a single protein communicating via intramolecular phosphotransfer. Here, we report how evolutionary relationships between hybrid HKs can be used as predictors of potential intermolecular and intramolecular interactions (‘phylogenetic promiscuity’). We used domain-swap genes chimeras to investigate the specificity of phosphotransfer within hybrid HKs of the GacS–GacA multikinase network of Pseudomonas brassicacearum. The receiver domain of GacS was replaced with those from nine donor hybrid HKs. Three chimeras with receivers from other hybrid HKs demonstrated correct functioning through complementation of a gacS mutant, which was dependent on strains having a functional gacA. Formation of functional chimeras was predictable on the basis of evolutionary heritage, and raises the possibility that HKs sharing a common ancestor with GacS might remain components of the contemporary GacS network. The results also demonstrate that understanding the evolutionary heritage of signaling domains in sophisticated networks allows their rational rewiring by simple domain transplantation, with implications for the creation of designer networks and inference of functional interactions.

KW - Article

KW - /631/326

KW - /631/326/41

KW - /631/326/41/2529

KW - /631/114

KW - /631/114/2114

KW - article

U2 - 10.1038/s41598-021-91260-w

DO - 10.1038/s41598-021-91260-w

M3 - Article

C2 - 34083699

VL - 11

SP - 11763

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

ER -

Show download statistics
View graph of relations
Citation formats