Environmental monitoring of Mycobacterium bovis in badger feces and badger sett soil by real-time PCR, as confirmed by immunofluorescence, immunocapture, and cultivation

Authors Organisations
  • F. P. Sweeney(Author)
    University of Warwick
  • O. Courtenay(Author)
    University of Warwick
  • V. Hibberd(Author)
    University of Warwick
  • Glyn Hewinson(Author)
    Animal and Plant Health Agency
  • L. A. Reilly(Author)
    University of Warwick
  • W. H. Gaze(Author)
    University of Warwick
  • E. M.H. Wellington(Author)
    University of Warwick
Type Article
Original languageEnglish
Pages (from-to)7471-7473
Number of pages3
JournalApplied and Environmental Microbiology
Volume73
Issue number22
Early online date28 Sep 2007
DOI
Publication statusPublished - 01 Nov 2007
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Abstract

Real-time PCR was used to detect and quantify Mycobacterium bovis cells in naturally infected soil and badger feces. Immunomagnetic capture, immunofluorescence, and selective culture confirmed species identification and cell viability. These techniques will prove useful for monitoring M. bovis in the environment and for elucidating transmission routes between wildlife and cattle.