Development of microsatellites for the peat moss Sphagnum capillifolium using ISSR cloning

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Type Article
Original languageEnglish
Pages (from-to)254-256
Number of pages3
JournalMolecular Ecology Notes
Volume7
Issue number2
DOI
Publication statusPublished - Mar 2007
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Abstract

Sphagnum mosses are major components of peat bogs but populations of many species are under threat due to habitat fragmentation resulting from the cutting of peat for fuel. We have used an intersimple sequence repeat (ISSR)-based cloning method to develop nine polymorphic nuclear microsatellites for the peat moss species Sphagnum capillifolium. Between three and seven alleles per locus were detected in a sample of 48 haploid gametophytes and levels of gene diversity ranged from 0.5391 to 0.7960. These represent the first microsatellite markers developed for this important genus and most also exhibited cross-species amplification across a range of common Sphagnum species.

Keywords

  • bryophyte, microsatellite, moss, peat bogs, simple sequence repeat, Sphagnum capillifolium