Development of a minimal KASP marker panel suitable for characterising apple collections

Authors Organisations
  • Mark O. Winfield(Author)
    Scottish Crop Research Institute
  • Amanda Burridge(Author)
    University of Bristol
  • Matthew Ordidge(Author)
    University of Reading
  • Helen Harper(Author)
    University of Bristol
  • Paul Wilkinson(Author)
    University of Bristol
  • Danny Thorogood(Author)
  • Elizabeth Copas(Author)
  • Keith Edwards(Author)
    University of Bristol
  • Gary L. Barker(Author)
    University of Bristol
Type Article
Original languageEnglish
JournalPLoS One
Publication statusAccepted/In press - 12 Nov 2020
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Abstract

Accurate identification of named accessions in germplasm collections is extremely important, especially for vegetatively propagated crops which are expensive to maintain. Thus, an inexpensive, reliable, and rapid genotyping method is essential because it avoids the need for laborious and time-consuming morphological comparisons. Single Nucleotide Polymorphism (SNP) marker panels containing large numbers of SNPs have been developed for many crop species, but such panels are much too large for basic cultivar identification. Here, we have identified a minimum set of SNP markers sufficient to distinguish apple cultivars held in the English and Welsh national collections providing a cheaper and automatable alternative to the markers currently used by the community. We show that SNP genotyping with a small set of well selected markers is equally efficient as microsatellites for the identification of apple cultivars and has the added advantage of automation and reduced cost when screening large numbers of samples.

Keywords

  • Apple, bioinformatics, genotyping, KASP®, MUNQ Codes, SNPs